Fignl1 (NM_001163359) Mouse Tagged ORF Clone
CAT#: MR216576
- TrueORF®
Fignl1 (Myc-DDK-tagged) - Mouse fidgetin-like 1 (Fignl1), transcript variant 1
ORF Plasmid: tGFP
"NM_001163359" in other vectors (4)
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CNY 5,000.00
CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216576 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGTCCAGCTCCATGTCTGTGGAGACGACTAGGTCTGTGCAGGTGGACGAATGGCAGAAGAATT ACTGTGTGGTTACATCCAGCATATGTACACCAAAGCAGAAGGCCGATGCATACCGTGCACTACTACTGCA TATTCAGTATGCATATGCCAACTCCGAGATCTCTCAGGTCTTTGCTACCAACCTGTTCAAAAGGTATACA GAAAAATACTCTGCAATTATTGATTCTGACAATGTTGTAACTGGCTTGAATAACTATGCAGAGAGCATTT TTGCTTTGGCAGGATCTCGACAGGCTGACAGTAACAAGTGGCAGTCTGGATTGTCAATAGATAATGTTTT CAAAATGAGTTGTGTACAGGAGATGATGCAGGCTGGCAAGAAATTTGAAGAGTCTCTGTTGGAACCTGCT GATGCATCAGTAGTCCTGTGTAAAGAGCCCACCGCCTTTGAGGTTCCTCAGCTTAGTGTTTGTGGAGGTT CTGAAGACGCTGACATATTATCCAGTTCAGGTCATGACACAGATAAGACCCAAGCCATTCCAGGGAGCAG TCTGAGATGTTCCCCTTTTCAGAGTGCTCGGCTGCCTAAGGAAACTAATACCACTAAGACATGCCTCACC TCCTCAACATCTTTAGGTGAGTCAGCCACTGCAGCATTTCACATGACACCATTATTTGGAAACACCGAAA AGGACACTCAAAGCTTTCCTAAAACCAGCACAGGACTAAATATGTTCTTATCTAATCTGTCTTGTGTTCC TTCTGGCTGTGAAAACCCTCAAGAAAGGAAGGCTTTTAATGACTCTGACATCATTGACATACTTTCCAAT CCAACACTGAACAAGGCTCCTAGTAAAACAGAAGACAGAGGCCGAAGGGAAGATAATAGCCTGCCTACCT TTAAAACTGCAAAAGAACAATTATGGGTAGATCAAAAGAAAAAGGGCCATCAATCCCAGCATACATCTAA ATCTTCTAATGGTGTTATGAAAAAGTCTCTGGGAGCTGGGAGGTCGAGAGGGATATTTGGCAAGTTTGTT CCTCCTGTATCTAATAAGCAAGACGGAAGTGAGCAGCATGCCAAGAAGCACAAGTCTAGTAGGGCAGGGT CTGCAGAACCAGCACACCTCACTGATGATTGTCTGAAGAACGTGGAGCCAAGGATGGTTGAACTTATTAT GAATGAAATTATGGACCATGGGCCTCCAGTACATTGGGACGATATTGCTGGAGTAGAATTTGCCAAAGCC ACAATAAAGGAAATCGTTGTGTGGCCCATGATGAGGCCAGATATCTTTACTGGATTGCGAGGGCCCCCTA AAGGAATTCTGCTCTTTGGCCCTCCAGGGACTGGTAAAACTCTGATTGGCAAGTGCATTGCTAGCCAGTC TGGAGCAACATTCTTCAGCATCTCTGCTTCATCGTTGACTTCTAAGTGGGTAGGTGAGGGAGAAAAAATG GTCCGTGCACTGTTTGCTGTTGCCAGGTGTCAGCAGCCAGCTGTCATATTTATTGATGAAATTGATTCTT TATTGTCTCAACGAGGAGATGGTGAACATGAATCTTCAAGAAGGATAAAAACGGAATTTTTAGTTCAGTT AGATGGAGCAACCACATCTTCTGAAGACCGGATTCTTGTGGTGGGAGCTACAAATCGGCCCCAAGAGATT GATGAAGCTGCCCGGAGAAGATTGGTGAAAAGACTTTATATTCCCCTCCCAGAAGCTTCAGCCAGGAAAC AGATAGTAGGTAATCTAATGTCTAAGGAGCAATGTTGTCTCAGTGATGAAGAAACTGATCTGGTAGTGCA GCAGTCTGATGGGTTTTCTGGCGCAGATATGACACAGCTTTGCAGAGAGGCTTCTCTTGGTCCTATTCGC AGTTTGCACGCTGCTGACATTGCTACCATAAGTCCAGATCAAGTTCGACCAATAGCTTATATTGATTTTG AAAATGCTTTTAAAACTGTGCGACCTACTGTATCTCCAAAAGACTTGGAGCTTTATGAAAACTGGAATGA AACATTTGGTTGTGGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216576 protein sequence
Red=Cloning site Green=Tags(s) METSSSMSVETTRSVQVDEWQKNYCVVTSSICTPKQKADAYRALLLHIQYAYANSEISQVFATNLFKRYT EKYSAIIDSDNVVTGLNNYAESIFALAGSRQADSNKWQSGLSIDNVFKMSCVQEMMQAGKKFEESLLEPA DASVVLCKEPTAFEVPQLSVCGGSEDADILSSSGHDTDKTQAIPGSSLRCSPFQSARLPKETNTTKTCLT SSTSLGESATAAFHMTPLFGNTEKDTQSFPKTSTGLNMFLSNLSCVPSGCENPQERKAFNDSDIIDILSN PTLNKAPSKTEDRGRREDNSLPTFKTAKEQLWVDQKKKGHQSQHTSKSSNGVMKKSLGAGRSRGIFGKFV PPVSNKQDGSEQHAKKHKSSRAGSAEPAHLTDDCLKNVEPRMVELIMNEIMDHGPPVHWDDIAGVEFAKA TIKEIVVWPMMRPDIFTGLRGPPKGILLFGPPGTGKTLIGKCIASQSGATFFSISASSLTSKWVGEGEKM VRALFAVARCQQPAVIFIDEIDSLLSQRGDGEHESSRRIKTEFLVQLDGATTSSEDRILVVGATNRPQEI DEAARRRLVKRLYIPLPEASARKQIVGNLMSKEQCCLSDEETDLVVQQSDGFSGADMTQLCREASLGPIR SLHAADIATISPDQVRPIAYIDFENAFKTVRPTVSPKDLELYENWNETFGCGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163359 |
ORF Size | 2049 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163359.1, NP_001156831.1 |
RefSeq Size | 2974 bp |
RefSeq ORF | 2052 bp |
Locus ID | 60530 |
UniProt ID | Q8BPY9 |
MW | 74.9 kDa |
Gene Summary | Involved in DNA double-strand break (DBS) repair via homologous recombination (HR). Recruited at DSB sites independently of BRCA2, RAD51 and RAD51 paralogs in a H2AX-dependent manner. May regulate osteoblast proliferation and differentiation (PubMed:17352653). May play a role in the control of male meiosis dynamic (PubMed:22110678).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220435 | Fignl1 (untagged) - Mouse fidgetin-like 1 (Fignl1), transcript variant 1, (10ug) |
CNY 5,512.00 |
|
MG216576 | Fignl1 (tGFP-tagged) - Mouse fidgetin-like 1 (Fignl1) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
MR216576L3 | Lenti ORF clone of Fignl1 (Myc-DDK-tagged) - Mouse fidgetin-like 1 (Fignl1), transcript variant 1 |
CNY 7,700.00 |
|
MR216576L4 | Lenti ORF clone of Fignl1 (mGFP-tagged) - Mouse fidgetin-like 1 (Fignl1), transcript variant 1 |
CNY 7,700.00 |