Dnal1 (NM_028821) Mouse Tagged ORF Clone
CAT#: MR216479
- TrueORF®
Dnalc1 (Myc-DDK-tagged) - Mouse dynein, axonemal, light chain 1 (Dnalc1)
ORF Plasmid: tGFP
"NM_028821" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1700010H15Rik; AW121714; Dnalc1; E330027P08Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216479 representing NM_028821
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAAGCAACAACGATCAAAGAAGCCCTGTCCAGATGGGAAGAAAAGACTGGTCAGAAGCCATCTG ATGCCAAAGAAATCAAGCTGTATGCCCAGATTCCCCCCATAGAGAAGATGGACGCATCTCTATCCACACT TGGTAACTGCGAGAAGCTTTCCCTGTCTACAAACTGCATTGAAAAAATTGCCAACCTGAATGGCCTAAAA AACTTGAGGATTTTGTCCTTAGGAAGAAACAACATAAAGAACCTAAATGGACTGGAAGCAGTAGGAGAAA CACTGGAAGAACTGTGGATCTCCTACAATTTTATTGAGAAGTTGAAAGGAATCCATGTAATGAAGAAACT GAAGATTCTCTACATGTCTAATAACCTGGTAAAAGACTGGGCTGAGTTCCTGAAGCTGGCCGAGCTGCCG TGCCTGGAGGACCTGGTGTTCGTCGGTAATCCCCTGGAGGAGAAACACTCTGCCGAGGGCAACTGGATCG ATGAAGCAACCAAGAGAGTGCCCAAGCTGAAAAAGCTGGATGGTACTCCAGTAATTAAGGAGGATGAAGA AGAGGAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216479 representing NM_028821
Red=Cloning site Green=Tags(s) MAKATTIKEALSRWEEKTGQKPSDAKEIKLYAQIPPIEKMDASLSTLGNCEKLSLSTNCIEKIANLNGLK NLRILSLGRNNIKNLNGLEAVGETLEELWISYNFIEKLKGIHVMKKLKILYMSNNLVKDWAEFLKLAELP CLEDLVFVGNPLEEKHSAEGNWIDEATKRVPKLKKLDGTPVIKEDEEEES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_028821 |
ORF Size | 570 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028821.3, NP_083097.2 |
RefSeq Size | 5792 bp |
RefSeq ORF | 573 bp |
Locus ID | 105000 |
UniProt ID | Q05A62 |
MW | 21.5 kDa |
Gene Summary | Part of the multisubunit axonemal ATPase complexes that generate the force for cilia motility and govern beat frequency (By similarity). Component of the outer arm dynein (ODA). May be involved in a mechanosensory feedback mechanism controlling ODA activity based on external conformational cues by tethering the outer arm dynein heavy chain (DNAH5) to the microtubule within the axoneme (By similarity). Important for ciliary function in the airways and for the function of the cilia that produce the nodal flow essential for the determination of the left-right asymmetry (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212110 | Dnal1 (untagged) - Mouse dynein, axonemal, light chain 1 (Dnalc1), (10ug) |
CNY 3,990.00 |
|
MG216479 | Dnalc1 (tGFP-tagged) - Mouse dynein axonemal light chain 1 (Dnalc1), (10ug) |
CNY 2,850.00 |
|
MR216479L3 | Lenti ORF clone of Dnalc1 (Myc-DDK-tagged) - Mouse dynein, axonemal, light chain 1 (Dnalc1) |
CNY 4,750.00 |
|
MR216479L4 | Lenti ORF clone of Dnalc1 (mGFP-tagged) - Mouse dynein, axonemal, light chain 1 (Dnalc1) |
CNY 4,750.00 |