Dnal1 (NM_028821) Mouse Untagged Clone

CAT#: MC212110

Dnal1 (untagged) - Mouse dynein, axonemal, light chain 1 (Dnalc1), (10ug)



  "NM_028821" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1700010H15Rik; AW121714; Dnalc1; E330027P08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212110 representing NM_028821
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAAGCAACAACGATCAAAGAAGCCCTGTCCAGATGGGAAGAAAAGACTGGTCAGAAGCCATCTG
ATGCCAAAGAAATCAAGCTGTATGCCCAGATTCCCCCCATAGAGAAGATGGACGCATCTCTATCCACACT
TGGTAACTGCGAGAAGCTTTCCCTGTCTACAAACTGCATTGAAAAAATTGCCAACCTGAATGGCCTAAAA
AACTTGAGGATTTTGTCCTTAGGAAGAAACAACATAAAGAACCTAAATGGACTGGAAGCAGTAGGAGAAA
CACTGGAAGAACTGTGGATCTCCTACAATTTTATTGAGAAGTTGAAAGGAATCCATGTAATGAAGAAACT
GAAGATTCTCTACATGTCTAATAACCTGGTAAAAGACTGGGCTGAGTTCCTGAAGCTGGCCGAGCTGCCG
TGCCTGGAGGACCTGGTGTTCGTCGGTAATCCCCTGGAGGAGAAACACTCTGCCGAGGGCAACTGGATCG
ATGAAGCAACCAAGAGAGTGCCCAAGCTGAAAAAGCTGGATGGTACTCCAGTAATTAAGGAGGATGAAGA
AGAGGAAAGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028821
Insert Size 573 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028821.3, NP_083097.2
RefSeq Size 5792 bp
RefSeq ORF 573 bp
Locus ID 105000
UniProt ID Q05A62
Gene Summary Part of the multisubunit axonemal ATPase complexes that generate the force for cilia motility and govern beat frequency (By similarity). Component of the outer arm dynein (ODA). May be involved in a mechanosensory feedback mechanism controlling ODA activity based on external conformational cues by tethering the outer arm dynein heavy chain (DNAH5) to the microtubule within the axoneme (By similarity). Important for ciliary function in the airways and for the function of the cilia that produce the nodal flow essential for the determination of the left-right asymmetry (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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