Nanos3 (NM_194059) Mouse Tagged ORF Clone
CAT#: MR216314
- TrueORF®
Nanos3 (Myc-DDK-tagged) - Mouse nanos homolog 3 (Drosophila) (Nanos3)
ORF Plasmid: tGFP
"NM_194059" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | nos3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216314 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACTTTCAATCTTTGGACAGATTACCTAGGTCTGGCAAGACTGGTTGGGGCTCTGCATAAGGAAG AGGAGCTTGATGTAAGGCTGGATCCCAAACCAGAGCCCAAGCCAAGTTCAGAAAGCCAGCAAGCCAGCAA GGAATCCTCTGCAGCTCCTGAACGCTTATGTTCATTCTGCAAACACAATGGCGAGTCCCGTGCCATCTAT CAGTCCCATGTCCTGAAGGATGAGGCAGGGCGGGTGCTGTGTCCCATTTTGCGGGATTATGTGTGTCCCC AGTGTGGGGCCACTCAGGAGCACGCCCACACTCGACGCTTTTGCCCACTCACCAGCCAGGGCTACACTTC TGTCTACTGCTACACCACCCGGAATTCTGCAGGCAAAAAGCTGACCCGGCCTGACAAGGCAAAGACACAG GATGCTGGCCACCGCCTTGGAGGAGAAGCAGCAGCAGGTGTCTATGCAGGTTCCAAAAGTGGCAGGAAGC CCCCTGGACCTTCACCCTCGGCCTGCTGTCCCTCCACTACGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216314 protein sequence
Red=Cloning site Green=Tags(s) MGTFNLWTDYLGLARLVGALHKEEELDVRLDPKPEPKPSSESQQASKESSAAPERLCSFCKHNGESRAIY QSHVLKDEAGRVLCPILRDYVCPQCGATQEHAHTRRFCPLTSQGYTSVYCYTTRNSAGKKLTRPDKAKTQ DAGHRLGGEAAAGVYAGSKSGRKPPGPSPSACCPSTTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_194059 |
ORF Size | 537 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_194059.1, NM_194059.2, NP_918948.1 |
RefSeq Size | 730 bp |
RefSeq ORF | 537 bp |
Locus ID | 244551 |
UniProt ID | P60324 |
MW | 19.2 kDa |
Gene Summary | Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
,Amadei, G;Zander, MA;Yang, G;Dumelie, JG;Vessey, JP;Lipshitz, HD;Smibert, CA;Kaplan, DR;Miller, FD;,
J. Neurosci.
,PubMed ID 26609159
[NANOS3]
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Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC213198 | Nanos3 (untagged) - Mouse nanos homolog 3 (Drosophila) (Nanos3), (10ug) |
CNY 3,990.00 |
|
MG216314 | Nanos3 (tGFP-tagged) - Mouse nanos homolog 3 (Drosophila) (Nanos3), (10ug) |
CNY 2,850.00 |
|
MR216314L3 | Lenti ORF clone of Nanos3 (Myc-DDK-tagged) - Mouse nanos homolog 3 (Drosophila) (Nanos3) |
CNY 4,750.00 |
|
MR216314L4 | Lenti ORF clone of Nanos3 (mGFP-tagged) - Mouse nanos homolog 3 (Drosophila) (Nanos3) |
CNY 4,750.00 |