Pelp1 (NM_029231) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4930563C04Rik; MNAR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR216109 representing NM_029231 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCCGTTCTTAGTGGGGCCTCTGCGGGCTCCCCAGCTGGGGCTCCTGGCGGGCCCGGGGGTC TCTCTGCGGTGGGATCCGGCCCGCGTCTGCGTCTGCTGCTGTTAGAGAGCATTTCCGGGTTGTTACAGCC GCGAACGGCGTCCCCCGTGGCCCCTGTGCATCCCCCAATCCAGTGGGCCCCGCATTTGCCCGGGCTCATG TGCTTATTGAGGCTGCATGGGACGGCAGGTGGGGCGCAGAATCTTTCAGCTCTTGGGGCATTGGTGAATC TCAGTAATGCACATCTTGGCTCCATCAAAACTCGGTTTGAAGGCCTGTGTCTGCTGTCCTTGTTGATAGG GGAGAGCCCCACAGAGTTATTCCAGCAACATTGTGTTTCTTGGCTTCGGAGCATCCAGCAGGTGCTGCAG TCTCAGGACTCACCGTCCACCATGGAGCTGGCTGTGGCTGTCCTGAGGGATCTGCTCCGACATGCATCCC AGCTTCCTACCCTGTTTCGGGACATCTCCACCAACCACCTTCCTGGGCTTCTTACTTCCTTGCTGGGCCT CAGACCAGAGTGTGAACAATCAGCTTTGGAGGGAATGAAGGCTTGTGTGACCTATTTTCCTCGGGCCTGT GGCTCTCTAAAAGGAAAGCTGGCTTCCTTTTTCCTGTCTCGATTGGATTCCCTGAACCCTCAGCTCCAAC AGCTGGCCTGCGAGTGTTACTCCAGGTTGCCCTCGTTAGGTGCTGGCTTTTCCCAGGGACTGAAGCACAC AGAGAACTGGGAACAGGAGCTGCACAGCCTGCTGACCTCACTGCACAGCTTACTGGGGTCCCTGTTTGAG GAAACAGAGCCTGCTCCTGTGCAGAGTGAAGGCCCTGGAATAGAGATGCTGCTTTCCCACTCAGAAGATG GTAACACCCATGTCCTCCTCCAGCTTCGGCAGAGGTTTTCAGGCCTGGCCCGTTGCCTGGGGCTCATGCT CAGCTCTGAGTTTGGGGCTCCTGTGTCCGTTCCTGTGCAGGAAATCCTGGACCTCATCTGCCGGATCCTT GGTATCAGTAGCAAGAATATTAACTTGCTTGGAGATGGTCCCCTCAGGTTGCTGCTGCTGCCTTCTCTGC ACCTTGAGGCCTTGGACTTGCTCTCTGCCCTGATCCTTGCGTGTGGAAGTCGACTTTTGCGTTTTGGTGC CCTGATCAGCCGGCTGCTTCCCCAGGTTCTCAATGCCTGGAGCACTGGGAGGGATACTCTAGCTCCAGGC CAGGAGAGGCCTTACAGCACCATTCGGACCAAAGTGTATGCTATCTTAGAGCTATGGGTGCAGGTTTGTG GGGCCTCAGCAGGTATGCTTCAGGGAGGAGCCTCTGGAGAAGCCTTGCTCACCCACCTGCTCAGTGACAT CTCCCCTCCAGCTGATGCTCTTAAGCTATGCAGTACCAGGGGAAGCTCTGATGGAGGTTTGCAAAGTGGG AAGCCAAGTGCTCCTAAGAAGCTCAAGCTCGATATGGGGGAAGCTTTGGCTCCACCCAGCCAAAGGAAAG GAGATAGGAATGCCAACAGTGACGTGTGCGCAGCTGCACTGAGAGGCCTCAGTCGGACCATCCTTATGTG CGGGCCTCTCATCAAGGAGGAGACTCACAGGAGACTTCATGATCTAGTCCTGCCCCTGGTCATGAGTGTC CAGCAGGGTGAGGTCCTGGGCAGCTCTCCTTACAACAGTTCCTGCTGTCGCCTCGGGCTCTACCGCCTGC TGCTGGCTTTATTGCTGGCACCTTCCCCTCGGTGCCCTCCTCCTCTTGCCTGTGCCCTGAAAGCTTTCTC TCTTGGCCAATGGGAAGATAGTCTTGAGGTCTCCTCTTTTTGCTCAGAAGCTCTGGTGACCTGTGCTGCT CTGACACACCCCCGAGTACCTCCTCTACAGAGCTCAGGCCCTGCCTGCCCCACTCCTGCCCCCGTCCCTC CTCCTGAGGCCCCATCTTCATTCAGGGCCCCGGCCTTCCATCCTCCAGGCCCCATGCCCTCCATAGGTGC TGTGCCATCCACAGGCCCATTACCCTCAGCAGGCCCCATTCCCACAGTAGGCTCTATGGCGTCCACAGGC CAAGTGCCCTCTCGCCCTGGACCTCCAGCAACAGCCAACCATTTAGGCCTCTCTGTCCCAGGCCTGGTAT CTGTTCCACCCAGGCTCCTTCCTGGCCCTGAAAACCACCGTGCAGGCTCCGGTGAGGATCCTGTCCTTGC TCCTAGTGGGACCCCTCCGCCTAGCATACCTCCAGATGAAACTTTTGGGGGGAGAGTACCTAGGCCAGCC TTTGTCCACTACGATAAGGAGGAGGCATCTGATGTAGAGATCTCCTTGGAAAGCGACTCTGATGACAGTG TGGTGATTGTACCTGAGGGGCTTCCCTCCCTGCCACCTGCACCACCCTCAGGCACTCCTCCCCCTGCGGC TCCTGCCGGCCCACCAACAGCCTCACCTCCTGTGCCAGCTAAGGAGGATTCTGAAGAACTGCCTGCAACC CCAGGGCCTCCACCCCCACCCCCACCCCCACCCCCACCGGCCTCTGGTCCTGTGACACTTCCTCCACCCC AGCTGGTCCCTGAAGGGACTCCTGGAGGGGGAGGACCCACAGCCATGGAAGAAGATTTGACAGTTATTAA TATCAACAGCAGTGATGAAGAGGAGGAGGAGGAAGAGGAAGAGGAGGAAGAAGATGAAGATGAGGAAGAA GAAGACTTTGAGGAAGAGGAAGAAGATGAAGAAGAATATTTTGAAGAGGAAGAAGAGGAAGAAGAGTTTG AGGAGGAATTTGAGGAGGAAGAAGGTGAATTAGAAGAGGAAGAAGAAGAGGAGGAAGAAGAGTTAGATGA GGTAGAAGATGTGGAGTTTGGTTCAGCAGGGGAGGTGGAAGAAGGTGGACCTCCACCCCCAACACTGCCA CCTGCTCTGCCACCTTCAGACTCGCCCAAAGTACAGCCTGAGGCAGAACCTGAACCTGGGCTCTTATTGG AAGTTGAGGAGCCAGGGCCAGAGGAGGTGCCTGGGCCTGAGACTGCTCCTACCCTGGCCCCTGAAGTGCT CCCCTCCCAGGAGGAGGGGGAGCAGGAAGTGGGAAGCCCTGCAGCAGGGCCACCTCAGGAGCTTGTTGAA GAGTCCTCTGCTCCCCCTGCCCTGCTGGAAGAGGGGACTGAGGGTGGAGGTGACAAAGTGCCACCCCCAC CGGAGACACCTGCAGAAGAAATGGAGACAGAAGCAGAAGTTCCAGCTCCTCAGGAAAAGGAGCAAGATGA TACAGCTGCCATGCTGGCTGATTTCATCGATTGTCCCCCTGACGATGAGAAGCCACCACCTGCTACAGAG CCTGACTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR216109 representing NM_029231 Red=Cloning site Green=Tags(s) MAAAVLSGASAGSPAGAPGGPGGLSAVGSGPRLRLLLLESISGLLQPRTASPVAPVHPPIQWAPHLPGLM CLLRLHGTAGGAQNLSALGALVNLSNAHLGSIKTRFEGLCLLSLLIGESPTELFQQHCVSWLRSIQQVLQ SQDSPSTMELAVAVLRDLLRHASQLPTLFRDISTNHLPGLLTSLLGLRPECEQSALEGMKACVTYFPRAC GSLKGKLASFFLSRLDSLNPQLQQLACECYSRLPSLGAGFSQGLKHTENWEQELHSLLTSLHSLLGSLFE ETEPAPVQSEGPGIEMLLSHSEDGNTHVLLQLRQRFSGLARCLGLMLSSEFGAPVSVPVQEILDLICRIL GISSKNINLLGDGPLRLLLLPSLHLEALDLLSALILACGSRLLRFGALISRLLPQVLNAWSTGRDTLAPG QERPYSTIRTKVYAILELWVQVCGASAGMLQGGASGEALLTHLLSDISPPADALKLCSTRGSSDGGLQSG KPSAPKKLKLDMGEALAPPSQRKGDRNANSDVCAAALRGLSRTILMCGPLIKEETHRRLHDLVLPLVMSV QQGEVLGSSPYNSSCCRLGLYRLLLALLLAPSPRCPPPLACALKAFSLGQWEDSLEVSSFCSEALVTCAA LTHPRVPPLQSSGPACPTPAPVPPPEAPSSFRAPAFHPPGPMPSIGAVPSTGPLPSAGPIPTVGSMASTG QVPSRPGPPATANHLGLSVPGLVSVPPRLLPGPENHRAGSGEDPVLAPSGTPPPSIPPDETFGGRVPRPA FVHYDKEEASDVEISLESDSDDSVVIVPEGLPSLPPAPPSGTPPPAAPAGPPTASPPVPAKEDSEELPAT PGPPPPPPPPPPPASGPVTLPPPQLVPEGTPGGGGPTAMEEDLTVININSSDEEEEEEEEEEEEDEDEEE EDFEEEEEDEEEYFEEEEEEEEFEEEFEEEEGELEEEEEEEEEELDEVEDVEFGSAGEVEEGGPPPPTLP PALPPSDSPKVQPEAEPEPGLLLEVEEPGPEEVPGPETAPTLAPEVLPSQEEGEQEVGSPAAGPPQELVE ESSAPPALLEEGTEGGGDKVPPPPETPAEEMETEAEVPAPQEKEQDDTAAMLADFIDCPPDDEKPPPATE PDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_029231
ORF Size	3369 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_029231.4, NP_083507.3
RefSeq Size	3463 bp
RefSeq ORF	3372 bp
Locus ID	75273
UniProt ID	Q9DBD5
MW	118.5 kDa
Gene Summary	Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K (By similarity). Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859). Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. Regulates pre-60S association of the critical remodeling factor MDN1 (By similarity).[UniProtKB/Swiss-Prot Function]

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