Khdrbs2 (NM_133235) Mouse Tagged ORF Clone

CAT#: MR215998

  • TrueORF®

Khdrbs2 (Myc-DDK-tagged) - Mouse KH domain containing, RNA binding, signal transduction associated 2 (Khdrbs2)

ORF Plasmid: DDK tGFP



  "NM_133235" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 6330586C16Rik; mSLM-1; Slim1; SLM; Slm-1; Slm1; Tg(LRRK2*R1441G)135Cjli; TG-RP135
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR215998 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGAAGAGAAATACTTGCCTGAGCTGATGGCAGAGAAGGATAGCCTGGATCCATCTTTTGTGCACG
CGTCGCGCCTTCTGGCGGAAGAAATTGAGAAATTTCAAGGTTCAGATGGGAAAAAGGAAGATGAAGAAAA
GAAATATCTCGATGTCATCAGCAACAAAAACATAAAGCTCTCTGAAAGAGTATTGATTCCTGTGAAACAG
TATCCAAAGTTCAATTTTGTGGGGAAATTGCTTGGACCAAGAGGAAACTCCTTGAAGAGGCTACAAGAAG
AAACGGGTGCTAAAATGTCTATCCTGGGCAAAGGGTCCATGCGAGATAAGACAAAGGAAGAAGAGCTGAG
GAAGAGTGGGGAGGCCAAGTATGCCCACCTGAGTGATGAGCTGCATGTATTAATTGAAGTGTTTGCTCCA
CCCGGGGAAGCTTATTCACGGATGAGTCATGCCTTGGAAGAGATTAAAAAATTCCTGGTTCCTGACTACA
ATGATGAAATTCGTCAAGAGCAACTCCGGGAGTTGTCTTACTTGAATGGCTCAGAAGAGTCTGGCCGGGG
CCGAGGTATTAGAGGCAGAGGGATCAGAATAACTCCCACAGCTCCATCAAGGGGCCGTGGCGGTGCTGTT
CCACCACCACCACCACCTGGACGAGGTGTGCTTACCCCTCGGGGGACCACTGTGACCCGTGGAGCTCTTC
CAGTGCCCCCAATAGCAAGAGGTGTCCCCACACCTCGAGCCCGGGGGACGGCAGCAGTACCAGGATACAG
AGCACCCCCACCTCCAGCTCATGATGCTTATGAAGAATATGGGTATGATGATGGCTATGGGGGTGAATAT
GATGACCAGACCTATGAGGCTTATGATAATAGCTACGTGACCCCAACACAAAGTGTGCCTGAATACTATG
ACTACGGTCATGGAGTAAACGAGGATGCCTACGACAGCTACGCACCAGAAGAATGGGCCACAACTCGCTC
CAGCCTGAAGGCACCACCACCAAGGTCAGCCAGAGGGGGATACAGGGAGCACCCCTATGGTAGATAT


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR215998 protein sequence
Red=Cloning site Green=Tags(s)

MGEEKYLPELMAEKDSLDPSFVHASRLLAEEIEKFQGSDGKKEDEEKKYLDVISNKNIKLSERVLIPVKQ
YPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKTKEEELRKSGEAKYAHLSDELHVLIEVFAP
PGEAYSRMSHALEEIKKFLVPDYNDEIRQEQLRELSYLNGSEESGRGRGIRGRGIRITPTAPSRGRGGAV
PPPPPPGRGVLTPRGTTVTRGALPVPPIARGVPTPRARGTAAVPGYRAPPPPAHDAYEEYGYDDGYGGEY
DDQTYEAYDNSYVTPTQSVPEYYDYGHGVNEDAYDSYAPEEWATTRSSLKAPPPRSARGGYREHPYGRY

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_133235
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133235.3
RefSeq Size 1363 bp
RefSeq ORF 1050 bp
Locus ID 170771
UniProt ID Q9WU01
MW 38.9 kDa
Gene Summary The protein encoded by this gene is similar to the src associated in mitosis, 68 kDa protein, which is an RNA-binding protein and a substrate for Src-family tyrosine kinases during mitosis. This protein has a KH RNA-binding motif and proline-rich motifs which may be SH2 and SH3 domain binding sites. A similar rat protein is an RNA-binding protein which is tyrosine phosphorylated by Src during mitosis. These studies also suggest that the rat protein may function as an adaptor protein for Src by binding the SH2 and SH3 domains of various other proteins. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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