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Sh3rf2 (NM_001146299) Mouse Tagged ORF Clone
CAT＃: MR215957
- TrueORF®
Sh3rf2 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1
ORF Plasmid: tGFP
"NM_001146299" in other vectors (4)
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CNY 5,392.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310046K19; 9130023G24Rik; AI452083; Ppp1r39; RNF158 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215957 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGATTTGACGTTACTTGATCTCCTGGAGTGCCCTGTGTGCTTTGAAAAGCTCGATGTCACAGCCA AAGTTCTCCCCTGCCAGCACACCTTCTGCAAACCATGTCTACAGAGGATTTTCAAGGCCCACAAGGAGCT GAGGTGCCCGGAATGCAGGACTCTGGTGTTCTGCAGCATCGAGGCTCTGCCAGCCAACCTGTTGCTCGTG CGTTTGCTGGATGGTGTGCGTTCCGGACAGAGCTCGTGGAAAGGAGGCTCCTTCCGAAGGCCTCGGATAC TGACCTTGCAGGACAACAGGAAAGCCAAGAGCAGCCCCAGAAGTCTACAGGCTAGCCCTTTCCGGCTGGT GCCCAGTGTCAGGATCCACATGGATGGGGTGCCTCGAGCCAAGGCCTTGTGCAACTACAGAGGGAAGAAT CCTGGCGACCTGAAGTTTAATAAAGGCGATGTCATCCTCCTTCGGAGACAGCTTGATGAGAACTGGTACC AGGGGGAGATTAATGGCGTCAGCGGGATCTTCCCGGCCAGCTCTGTGGAAGTCATCAAACAGCTGCCCCA ACCACCCCCACTCTGCAGAGCCCTCTACAACTTTGACCTTCGAGACAAGGATAAGAGTGAGAACCAGGAC TGCCTGACCTTCCTCAAGGATGATGTCATCACAGTGATCAGCCGTGTAGATGAGAACTGGGCAGAGGGAA AGCTCGGAGATAAAGTGGGCATCTTCCCCATCTTGTTTGTAGAGCCAAACGTCTCGGCAAGACACCTCCT AGAGAACAAAGGACATCAGTTATCACGCACAAGACACCTGTCCCTGATGTCCTCCCCATCCAGAGGCAAA GCGACCAACACATCCTCGCTCCGAAAGAGCCCAGGGTCCAGGAGGAAGGGATCTGGGCAGTTCTCCATGA CTACAGCTTTGAACACACTCAACCGTACAGTCCATTCTCCTGAAGGCCACCAGATGGTGGAAATCAGCAC CCCGATGCTCATCAGCTCTAACAGCCCCTCTGTGCTCACCCAACATGGGGACAAGGCAGACTTCCCTACC AGCTCTACAGGACAGGTCAGCAGTTCTCAGCCTGCACCTGCCTCCCCGGGACATTCCACAGCCATGGTCA GTGTGCCCAGCTCCCAGCAACACCTTTCAAATAACATGTTTGTAGCCCTACACACTTACTCAGCCCATAG ACCCGAAGAGCTGGACCTGCAGAAGGGAGAAGGCATCAGGGTTCTGGGCAAGTACCAGGATGGCTGGCTG AAGGGCCTCTCCTTACTCACTGGACGAACTGGCATCTTTCCAAGCGACTATGTCATCCCAGTCTTCAGTT CAACAGCTAGAAAGACATCTAGTTTTCCTGATTCCCGGAGCCCCACTGTCTGCACCACATGGGCGTTATC CACCTCCTCTGTGTCCTCCCAGGGTAGCTTTGCAGAAGGTGATCCCCGGCAAAGTGGACCCTTCAAATCC GTCTTTGTGCCCACTGCCGTAGTTAACCCTTCGAGGAGCACACCTGGCCCAGGGTCTTCTGGACAAGGGT CTCTTAGGAAAGTGCGGAGCAGCATGAGAAAAAATGGATCCCTGCAGAGACCAGTTCAATCCGGGATCCC CACTTTCATGGTGGGCTCCCTCAGGTGCAGTCCTACCATGGTGATCCGGCCTCAGAGGTTCCAGTTTTAT CCGCCACAGGGGATGACCCCTTCCCCGACACCGATAATGGTGGAGATGGGGTCTAAATCTATTTACACTG GGGAGCCTGCCCTGACCTGCATCAACAGAGGTAGCAAGACCAGGATCCACTCAGCAGGCAATTCCATCAT CATGGAAGGCAAAGAAACGCCCATCAAGAGTGAACCACCACCCAAGCCGCCTGCATCTGCGCCACCCTCC ATCCTCGTGAAACCAGAAAATTCAAAGAATGGCACTGAAAAGCAAGTCAAAACCGTGAGATTTCAGAATT ACAGCCCTCCGCCCACCAAACACTCTGCCTCCAGTCCCACCTCTGGAAAGCACGACCATCCAGCCACCTT GAAGGGGTCCCAGCATGAAGCAGTCTCCTCGGGAGGAGAGATGACCATTCTATTTGCCCACCGAAGTGGC TGCCACTCCGGGCAACAAACAGACCTTCGGAGAAAGTCAGCCTTTGGCAAGACCATGCCCTTATTGTCCA CTGCTTCAGCCACACAGACCTTGTTTCCCAGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215957 protein sequence
Red=Cloning site Green=Tags(s) MDDLTLLDLLECPVCFEKLDVTAKVLPCQHTFCKPCLQRIFKAHKELRCPECRTLVFCSIEALPANLLLV RLLDGVRSGQSSWKGGSFRRPRILTLQDNRKAKSSPRSLQASPFRLVPSVRIHMDGVPRAKALCNYRGKN PGDLKFNKGDVILLRRQLDENWYQGEINGVSGIFPASSVEVIKQLPQPPPLCRALYNFDLRDKDKSENQD CLTFLKDDVITVISRVDENWAEGKLGDKVGIFPILFVEPNVSARHLLENKGHQLSRTRHLSLMSSPSRGK ATNTSSLRKSPGSRRKGSGQFSMTTALNTLNRTVHSPEGHQMVEISTPMLISSNSPSVLTQHGDKADFPT SSTGQVSSSQPAPASPGHSTAMVSVPSSQQHLSNNMFVALHTYSAHRPEELDLQKGEGIRVLGKYQDGWL KGLSLLTGRTGIFPSDYVIPVFSSTARKTSSFPDSRSPTVCTTWALSTSSVSSQGSFAEGDPRQSGPFKS VFVPTAVVNPSRSTPGPGSSGQGSLRKVRSSMRKNGSLQRPVQSGIPTFMVGSLRCSPTMVIRPQRFQFY PPQGMTPSPTPIMVEMGSKSIYTGEPALTCINRGSKTRIHSAGNSIIMEGKETPIKSEPPPKPPASAPPS ILVKPENSKNGTEKQVKTVRFQNYSPPPTKHSASSPTSGKHDHPATLKGSQHEAVSSGGEMTILFAHRSG CHSGQQTDLRRKSAFGKTMPLLSTASATQTLFPSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146299 |
| ORF Size | 2208 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001146299.1, NP_001139771.1 |
| RefSeq Size | 4784 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 269016 |
| UniProt ID | Q8BZT2 |
| MW | 80.1 kDa |
| Gene Summary | Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221203 | Sh3rf2 (untagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1, (10ug) |
CNY 9,500.00 |
|
| MG215957 | Sh3rf2 (tGFP-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
| MR215957L3 | Lenti ORF clone of Sh3rf2 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1 |
CNY 7,700.00 |
|
| MR215957L4 | Lenti ORF clone of Sh3rf2 (mGFP-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1 |
CNY 7,700.00 |
