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Prame (NM_029459) Mouse Tagged ORF Clone
CAT＃: MR214968
- TrueORF®
Prame (Myc-DDK-tagged) - Mouse preferentially expressed antigen in melanoma (Prame)
ORF Plasmid: tGFP
"NM_029459" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930534P07Rik; RP23-250F8.3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR214968 representing NM_029459
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGGATGAAAGAGACCCATCCTCGCTTTTGGATCTGGCTATACAGAGTCTACTAAGTAATGAGC TTGTAGCAATTCATTCTCTGGGGGAGATCCCAAGGGAGCTTTTTGTTCCATTGTTCTCTGCTGCCTTCAC GGGAGGATATAGGAAGATACTGACTTCAATGGTGAAGATTTGGCCTTTTACCTGTCTCCACATTGGAACA TTAAGTGTACAGGAACCCCAGCGTGAACTCCTGAAAGCCATGGTTGAGAGTCTTCAGTTTCTTCCTGCCC AGGACTGTTCTTCTGGGGGCCCTAAGTTGAGGATCCTAGATGTAAGGCAGGGTGTTGACTGCAAGACAAC ATGCCCTGATTTTGGTGCCAGATCTCCAACTTGTTTTCATGGTTGTACTCACTCTGTACACTCTATTCTG AAGTTAGAAAGCCAGTACAGCATTGTAGATCTAAAGCCCGAGAGTCAGTCTGCAATCCAGCCTATGGAAC TACTAGTAGACCTTTCCCTTGATGGTACCTTGAGAGAAAGGGAATTTTTTGCTTTGCTTCTGAATAAAGT ACAGCAGAGCTCAGGGTCTTTGCACCTCTGCTGCCGAGATCTACAAATTGATAGATTTTCTTATGCCAAA AACGCTCTGAAGTTCCTCGATCTAACTTGCATTCAGAACCTGACAGTTGATCAGGCTTCACTGAGTGAAG TCACCACTCTTCTGGCTCGCATGATCTATCTGGACAGCCTGAGTCTCTCTAAAATCACTTATAGATCTTT GCATGGGAAAGTCTTCCGAGTGTTCCTCAACTATCTTGGGCGGATGAACTGCCTGAAAGAGCTCAACCTG TCTTCCTTTAGCCTCACAGACCATCTGGATAGCCTCCTCAGAGCCTTACCACCTAATTTGGATTTCTTGT ATCTGCCGTTCTGTGAAATTTCTTACAGAGATCTCAAATTTCTATCCCAGAGTGCTCAGGCCACCCACTT AAAGCTGTTGAATCTTAGTAACAACCCAATGTATTGGGATGATTGTGGGCCTTTTCAGACTCTTTTGCAG AAGCTCTCAGATACCTTGCAGCATCTGGCCATAAACCATTGCCATTTAACAGATGCCATACTCTCTGCTA TTCTGCCAGCACTATCTAAGTGTTCCCATCTCCGTGTGATTAGCTTTGTCTCTAACCCCATTTCAATGCC TATGCTCCTGAAAATTCTTCATTACTTAACACCTTTGATGGAGCTGAAATACGTGATTTACCCTATCCCT ATACATTGCTATGAACAATGGCAATTTCATGGCAGATTAGACCGGCAGAAGCTCACCGATGTCCAAGCAC AACTGAAGGCAATGCTACAAGCAGCAAAAAGGAGTGACATGAACTGGATCACTTATTCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR214968 representing NM_029459
Red=Cloning site Green=Tags(s) MMMDERDPSSLLDLAIQSLLSNELVAIHSLGEIPRELFVPLFSAAFTGGYRKILTSMVKIWPFTCLHIGT LSVQEPQRELLKAMVESLQFLPAQDCSSGGPKLRILDVRQGVDCKTTCPDFGARSPTCFHGCTHSVHSIL KLESQYSIVDLKPESQSAIQPMELLVDLSLDGTLREREFFALLLNKVQQSSGSLHLCCRDLQIDRFSYAK NALKFLDLTCIQNLTVDQASLSEVTTLLARMIYLDSLSLSKITYRSLHGKVFRVFLNYLGRMNCLKELNL SSFSLTDHLDSLLRALPPNLDFLYLPFCEISYRDLKFLSQSAQATHLKLLNLSNNPMYWDDCGPFQTLLQ KLSDTLQHLAINHCHLTDAILSAILPALSKCSHLRVISFVSNPISMPMLLKILHYLTPLMELKYVIYPIP IHCYEQWQFHGRLDRQKLTDVQAQLKAMLQAAKRSDMNWITYSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029459 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029459.2, NP_083735.2 |
| RefSeq Size | 2267 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 75829 |
| MW | 52.7 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216339 | Prame (untagged) - Mouse preferentially expressed antigen in melanoma (Prame), (10ug) |
CNY 6,080.00 |
|
| MG214968 | Prame (tGFP-tagged) - Mouse preferentially expressed antigen in melanoma (Prame), (10ug) |
CNY 3,710.00 |
|
| MR214968L3 | Lenti ORF clone of Prame (Myc-DDK-tagged) - Mouse preferentially expressed antigen in melanoma (Prame) |
CNY 5,230.00 |
|
| MR214968L4 | Lenti ORF clone of Prame (mGFP-tagged) - Mouse preferentially expressed antigen in melanoma (Prame) |
CNY 5,230.00 |
