4930432K21Rik (NM_001163752) Mouse Tagged Lenti ORF Clone
CAT#: MR214541L4
- LentiORF®
Lenti ORF clone of 4930432K21Rik (mGFP-tagged) - Mouse RIKEN cDNA 4930432K21 gene (4930432K21Rik), transcript variant 2
"NM_001163752" in other vectors (3)
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CNY 7,510.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR214541).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163752 |
ORF Size | 1695 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163752.1, NP_001157224.1 |
RefSeq Size | 2389 bp |
RefSeq ORF | 1698 bp |
Locus ID | 74666 |
UniProt ID | Q6DIA7 |
Gene Summary | Meiotic recombination factor component of recombination bridges involved in meiotic double-strand break repair (PubMed:32463460, PubMed:32460033). Modulates the localization of recombinases DMC1:RAD51 to meiotic double-strand break (DSB) sites through the interaction with and stabilization of the BRCA2:HSF2BP complex during meiotic recombination (PubMed:32460033, PubMed:32463460). Indispensable for the DSB repair, homologous synapsis, and crossover formation that are needed for progression past metaphase I, is essential for spermatogenesis and male fertility (PubMed:32463460, PubMed:32460033).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG214541 | 4930432K21Rik (tGFP-tagged) - Mouse RIKEN cDNA 4930432K21 gene (4930432K21Rik) transcript variant 2, (10ug) |
CNY 6,180.00 |
|
MR214541 | 4930432K21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930432K21 gene (4930432K21Rik), transcript variant 2 |
CNY 5,610.00 |
|
MR214541L3 | Lenti ORF clone of 4930432K21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930432K21 gene (4930432K21Rik), transcript variant 2 |
CNY 7,510.00 |
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