Lrif1 (NM_001039478) Mouse Tagged ORF Clone

CAT#: MR213437

  • TrueORF®

Lrif1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4933421E11 gene (4933421E11Rik), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001039478" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,570.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2010012G17Rik; 4933421E11Rik; AI450568
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR213437 representing NM_001039478
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGCGGGACCCGCGCAAGGAGCAGGGCAAACAAGGCAAAGCCGACGCAGTATGAGATGGCATCAA
TAGTAAAAAAGGAAATTCAAGAAAAAGGAAATAACAAGAAATATTCTCAAGGAAGCCATACTAATATTAA
GGCATCATGTCTGAAGAATGATGCTGAATTTAAAAAGCTATTTGGTCTCACTAAAGATTTGAGAGTATGT
CTTACTCGAATTCCTGACCATCTGAGCTCTGGAAAAAGTTTTAATTCCTTTAACAGTTTGATGAAAAGTA
GTTCTTACAAAGATGCAAACATTGTGGTGAAGAAAGAAGAAAAAAAACAGAGTTTCTCTAAGAAAAGAAA
AGCGGAAACCATGAAGATGGGTAACACAAAAAAGATAAAAATTGAGAATGCTGATGACACGGTCATGAGC
ATCATGAATGGAACTGATGTTGCTAGTTCCCAACCTCTCAGTAGTATTTTACCAACATCAGACATATCAC
AACATAACATTGTCACAAGCCATAGTACAACCAGGGAAGACAAGAGAACTGAGGCAGAACACTGTTCTCA
TGAGAAGCAGGAAAAAGGCACATTAAGTTCAAGCACATCTTTTGAACAAAGTACTTTTTTAAATAAAAAC
TTTATGGAAGATATTTTTCCAGTGACACCACCAGAACTAGAAGAAACCATCAGAGATGAAAAAATAAGAA
GACTTAAGCAAATTTTAAGAGAAAAAGAAGCAGCTCTTGAAGAATTGCGTAAGAAGATGTACCAAAAACA
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR213437 representing NM_001039478
Red=Cloning site Green=Tags(s)

MAGGTRARSRANKAKPTQYEMASIVKKEIQEKGNNKKYSQGSHTNIKASCLKNDAEFKKLFGLTKDLRVC
LTRIPDHLSSGKSFNSFNSLMKSSSYKDANIVVKKEEKKQSFSKKRKAETMKMGNTKKIKIENADDTVMS
IMNGTDVASSQPLSSILPTSDISQHNIVTSHSTTREDKRTEAEHCSHEKQEKGTLSSSTSFEQSTFLNKN
FMEDIFPVTPPELEETIRDEKIRRLKQILREKEAALEELRKKMYQKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001039478
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001039478.1, NP_001034567.1
RefSeq Size 1612 bp
RefSeq ORF 774 bp
Locus ID 321000
MW 29.7 kDa
Gene Summary Together with SMCHD1, involved in chromosome X inactivation in females by promoting the compaction of heterochromatin. Also able to repress the ligand-induced transcriptional activity of retinoic acid receptor alpha (RARA), possibly through direct recruitment of histone deacetylases.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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