Krtap27-1 (NM_001163105) Mouse Tagged ORF Clone

CAT#: MR213354

  • TrueORF®

Krtap27 (Myc-DDK-tagged) - Mouse keratin associated protein 27-1 (Krtap27-1)

ORF Plasmid: DDK tGFP



  "NM_001163105" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms EG239933
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR213354 representing NM_001163105
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCATAGCCATTGCCAGTCATTCAGGAGCTTGGGTAAAGCTCCGTCACTGTCTTCCATCACACACA
ACTCTAATCCTAAAAGCTTTGAAGATGGCATTGTCTTACCCAGCAGTTGTCACAGAAGAACCTGGCTCCT
GGACAACTTTCAAGAAACCTACAGTGAAACTTCTACCTGCCAACCAGCCAATTGTGGACAGAACCAGTGC
AAAGTGGACCCCTGTGTGCAAAGTTCCCAGAACTCCAGAGCTGAACAAACAATTTGTTCTAATTCCAAGA
CCTGTGAAAGAACAAGAGAAATATCTCCAGCTGTGCCAGAGAGTGCTTCTCAAACCTGCCAATCAAGGAA
TTATAAGCAAGTGGGTTTTGTAGCCCAGAGCTACCAACCTGCAAGTTACATGGCCAAGTGTTGCCCACTA
AAGTCTACAGCATTTGAAAGTTGCCAGACGTTGGAGTTTGAATCCAGCCCATGCTGCTCTCAGGTCTCTG
AGTCCAGGTCCTGTAATTCCTCCAGCAGTATTGTCTCTGGGCTACATCTTGTGGAATCTTCCAACACTTA
TGAGCCAACATGCTGTGTGACTGGTGGTTCACAAGTGCCTAGTAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR213354 representing NM_001163105
Red=Cloning site Green=Tags(s)

MLHSHCQSFRSLGKAPSLSSITHNSNPKSFEDGIVLPSSCHRRTWLLDNFQETYSETSTCQPANCGQNQC
KVDPCVQSSQNSRAEQTICSNSKTCERTREISPAVPESASQTCQSRNYKQVGFVAQSYQPASYMAKCCPL
KSTAFESCQTLEFESSPCCSQVSESRSCNSSSSIVSGLHLVESSNTYEPTCCVTGGSQVPSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001163105
ORF Size 606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001163105.2
RefSeq Size 609 bp
RefSeq ORF 609 bp
Locus ID 239933
MW 22 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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