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Rcor1 (NM_198023) Mouse Tagged ORF Clone
CAT＃: MR212462
- TrueORF®
Rcor1 (Myc-DDK-tagged) - Mouse REST corepressor 1 (Rcor1)
ORF Plasmid: tGFP
"NM_198023" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730409O11; 6720480E22Rik; AU042633; D12Wsu95e; mKIAA0071; Rocr1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212462 representing NM_198023
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGTGGGACCCCAGTACCAAGCGGCGGTGCCCGACTTCGATCCTGCCAAACTGGCAAGGCGCAGTC AAGAACGAGACAATCTTGGCATGTTGGTCTGGTCACCTAATCAGAGTCTTTCTGAAGCAAAACTGGACGA GTACATCGCTATCGCCAAAGAGAAGCACGGGTACAACATGGAGCAGGCTCTTGGGATGCTCTTTTGGCAT AAGCATAATATTGAAAAATCATTGGCCGACTTGCCCAACTTCACCCCCTTTCCAGATGAGTGGACTGTAG AAGACAAAGTCTTATTTGAGCAAGCCTTTAGTTTTCATGGGAAAACTTTTCATAGGATCCAGCAAATGCT TCCTGATAAATCCATAGCAAGTTTGGTGAAGTTTTACTACTCTTGGAAGAAAACTAGAACTAAAACAAGT GTGATGGATCGCCATGCTCGGAAACAGAAGCGGGAGCGGGAGGAAAGTGAGGATGAACTGGAAGAAACAA ACGGCAGTAATCCCGTTGACATTGAGATTGACCCAAACAAGGAAAGCAAAAAGGAGGTGCCCCCTACAGA GACAGTTCCTCAGGTCAAAAAAGAAAAGCACAGCACACAAGCTAAAAATAGAGCAAAAAGGAAACCTCCG AAAGGCATGTTTCTTTCTCAAGAAGATGTGGAGGCTGTGTCTGCTAATGCTACTGCTGCGACCACGGTGC TGAGACAGCTGGACATGGAGCTGGTCTCCATCAAACGACAGATTCAGAACATCAAGCAGACGAACAGTGC TCTTAAAGAGAAGCTTGATGGTGGGATAGAGCCTTACCGACTCCCAGAGGTTATTCAAAAATGCAACGCT CGCTGGACAACGGAAGAGCAGCTTCTCGCCGTACAAGCCATTAGGAAATATGGGCGAGATTTTCAGGCGA TCTCAGATGTGATTGGGAACAAGTCAGTTGTTCAGGTGAAAAACTTTTTTGTAAATTATCGACGCCGCTT CAACATAGATGAAGTTTTACAAGAATGGGAAGCAGAACATGGGAAAGACGAGACCAATGGACCTGCTAAC CAGAAACCTGTCAAGTCCCCAGAAAGCTCCATCAAGATCCCGGAGGAGGAAGATGAGGCTGCTTCTGTCC TCGACGTAAGATACGCATCTGCCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212462 representing NM_198023
Red=Cloning site Green=Tags(s) MRVGPQYQAAVPDFDPAKLARRSQERDNLGMLVWSPNQSLSEAKLDEYIAIAKEKHGYNMEQALGMLFWH KHNIEKSLADLPNFTPFPDEWTVEDKVLFEQAFSFHGKTFHRIQQMLPDKSIASLVKFYYSWKKTRTKTS VMDRHARKQKREREESEDELEETNGSNPVDIEIDPNKESKKEVPPTETVPQVKKEKHSTQAKNRAKRKPP KGMFLSQEDVEAVSANATAATTVLRQLDMELVSIKRQIQNIKQTNSALKEKLDGGIEPYRLPEVIQKCNA RWTTEEQLLAVQAIRKYGRDFQAISDVIGNKSVVQVKNFFVNYRRRFNIDEVLQEWEAEHGKDETNGPAN QKPVKSPESSIKIPEEEDEAASVLDVRYASAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198023 |
| ORF Size | 1146 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198023.2, NP_932140.1 |
| RefSeq Size | 2725 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 217864 |
| UniProt ID | Q8CFE3 |
| MW | 44.3 kDa |
| Gene Summary | Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212674 | Rcor1 (untagged) - Mouse REST corepressor 1 (Rcor1), (10ug) |
CNY 3,990.00 |
|
| MG212462 | Rcor1 (tGFP-tagged) - Mouse REST corepressor 1 (Rcor1), (10ug) |
CNY 7,088.00 |
|
| MR212462L3 | Lenti ORF clone of Rcor1 (Myc-DDK-tagged) - Mouse REST corepressor 1 (Rcor1) |
CNY 5,890.00 |
|
| MR212462L4 | Lenti ORF clone of Rcor1 (mGFP-tagged) - Mouse REST corepressor 1 (Rcor1) |
CNY 5,890.00 |
