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Nkx6-1 (NM_144955) Mouse Tagged ORF Clone
CAT＃: MR212146
- TrueORF®
Nkx6 (Myc-DDK-tagged) - Mouse NK6 homeobox 1 (Nkx6-1)
"NM_144955" in other vectors (3)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Nkx6.1; NKX6A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212146 representing NM_144955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGCTGTGGGGGCGATGGAGGGCCCTCGGCAGAGCGCGTTCCTGCTCAGCAGCCCGCCCCTGGCCG CCCTGCACAGTATGGCCGAGATGAAGACCCCGCTCTACCCCGCCGCTTATCCCCCGCTGCCCACCGGGCC CCCCTCCTCCTCGTCCTCGTCCTCCTCGTCCTCGTCGCCCTCCCCACCTTTGGGCTCACATAACCCGGGC GGCTTGAAGCCCCCGGCCGCGGGGGGCCTCTCGTCCCTGGGCAGTCCCCCGCAGCAGCTTTCGGCGGCCA CCCCACACGGCATCAACGACATCCTGAGCCGGCCCTCTATGCCGGTGGCCTCGGGGGCCGCCCTGCCCTC CGCCTCGCCCTCCGGGTCTTCCTCCTCCTCCTCCTCGTCCGCCTCCGCCACCTCGGCCTCTGCGGCGGCC GCCGCCGCCGCTGCTGCTGCCGCCGCTGCCGCCTCGTCGCCCGCTGGGCTGCTGGCCGGCCTGCCCCGCT TCAGCAGCCTGAGCCCTCCGCCACCGCCGCCCGGGCTCTACTTTAGCCCCAGCGCCGCGGCTGTGGCCGC CGTGGGCCGGTACCCCAAGCCCCTGGCCGAGCTGCCCGGTCGGACGCCCATCTTCTGGCCCGGAGTGATG CAGAGTCCGCCGTGGAGGGACGCGCGCCTTGCCTGTACCCCCCATCAAGGATCCATTTTGTTGGACAAAG ATGGGAAGAGAAAACACACCAGACCCACGTTCTCTGGACAGCAAATCTTCGCCCTGGAGAAGACTTTCGA ACAAACGAAGTACTTGGCAGGACCAGAGAGAGCACGCTTGGCCTATTCTCTGGGGATGACGGAGAGTCAG GTCAAGGTCTGGTTCCAGAACCGCAGGACCAAGTGGAGAAAGAAGCACGCAGCCGAGATGGCCACGGCCA AGAAGAAGCAGGACTCGGAGACCGAGAGGCTCAAGGGGACTTCGGAGAATGAGGAGGATGACGACGATTA CAACAAACCTCTGGACCCGAACTCTGACGACGAGAAAATCACTCAGCTGCTGAAAAAGCACAAATCGAGC GGTGGCAGCCTCCTGCTGCACGCGTCGGAGGCCGAGGGCTCGTCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR212146 representing NM_144955
Red=Cloning site Green=Tags(s) MLAVGAMEGPRQSAFLLSSPPLAALHSMAEMKTPLYPAAYPPLPTGPPSSSSSSSSSSSPSPPLGSHNPG GLKPPAAGGLSSLGSPPQQLSAATPHGINDILSRPSMPVASGAALPSASPSGSSSSSSSSASATSASAAA AAAAAAAAAAASSPAGLLAGLPRFSSLSPPPPPPGLYFSPSAAAVAAVGRYPKPLAELPGRTPIFWPGVM QSPPWRDARLACTPHQGSILLDKDGKRKHTRPTFSGQQIFALEKTFEQTKYLAGPERARLAYSLGMTESQ VKVWFQNRRTKWRKKHAAEMATAKKKQDSETERLKGTSENEEDDDDYNKPLDPNSDDEKITQLLKKHKSS GGSLLLHASEAEGSS SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144955 |
| ORF Size | 1095 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144955.2, NP_659204.1 |
| RefSeq Size | 1808 bp |
| RefSeq ORF | 1098 bp |
| Locus ID | 18096 |
| UniProt ID | Q99MA9 |
| MW | 38.2 kDa |
| Gene Summary | Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Required for the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (PubMed:11076772). Involved in transcriptional regulation of the insulin gene. Together with NKX2-2 and IRX3, restricts the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208034 | Nkx6 (untagged) - Mouse NK6 homeobox 1 (Nkx6-1), (10ug) |
CNY 3,990.00 |
|
| MR212146L3 | Lenti ORF clone of Nkx6 (Myc-DDK-tagged) - Mouse NK6 homeobox 1 (Nkx6-1) |
CNY 5,890.00 |
|
| MR212146L4 | Lenti ORF clone of Nkx6 (mGFP-tagged) - Mouse NK6 homeobox 1 (Nkx6-1) |
CNY 5,890.00 |
