Plch1 (NM_183191) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BC042549; PLC-eta-1; PLCeta1; Plcl3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212059 representing NM_183191 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCTTGAAGTGTATAAAAACTTAAGTCCAGAAAAAGTTGAAAGATGCATGAGTGTAATGCAGT CCGGGACACAGATGATCAAACTGAAGCGTGGCACCAAAGGGCTCGTTCGCCTCTTTTACCTGGATGAGCA CCGGACCCGCCTCCGATGGCGACCCTCTCGGAAGAGTGAGAAGGCAAAAATACTTATTGACTCCATCTAC AAAGTCACCGAGGGCAGGCAGTCTGAAATATTCCACAGACAGGCTGAGGGGAACTTTGACCCTAGCTGCT GCTTTACCATCTACCATGGCAACCACATGGAGTCTCTGGACCTCATTACCTCCAACCCAGAGGAGGCACG CACCTGGATCACGGGCCTCAAGTATCTGATGGCTGGCATCAGTGATGAAGACTCCCTTGCCAAGAGGCAG AGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAACGGTGATGGCTTATTGAATA TTGAAGAGATTCACCAGCTGATGCATAAACTAAATGTCAATCTGCCCCGCAGAAAAGTCAGGCAAATGTT TCAGGAAGCAGATACAGATGAGAATCAGGGAACCTTGACATTTGAAGAGTTCTGCGTTTTTTACAAAATG ATGTCATTGAGACGAGACCTCTATTTGCTGCTCTTGAGCTACAGTGACAAGAAAGACCACCTGACTGTGG AGGAGCTGGCTCAGTTTTTGAAAGTGGAACAAAAGATGAGTAATGTGACACTGGACTATTGTCTTGACAT CATAATGAAGTTTGAAGTTTCTGAAGAAAACAAAGTGAAAAACGTCCTTGGTATAGAAGGCTTCACGAAC TTCATGCGTAGCCCTGCCTGTGACGTATTTAACCCGTTGCACCATGAAGTGTACCAAGACATGGATCAGC CCCTGTGCAACTACTACATTGCTTCATCTCACAACACATACCTGACTGGGGATCAGCTCCTTTCTCAATC TAAAGTGGATATGTATGCACGGGTGCTACAAGAGGGTTGTAGATGCGTGGAAGTTGACTGTTGGGATGGC CCAGATGGAGAGCCAGTGGTCCACCATGGTTATACTCTCACTTCAAAAATTCTCTTCAGAGATGTTGTGG AGACCATCAACAAGCATGCATTCGTGAAGAATGAGTTCCCGGTTATCCTGTCCATTGAGAACCACTGCAG CATTCAGCAGCAGAGGAAGATTGCTCAGTACCTGAAAGGTATATTGCAGGACAAACTGGACCTGTCTTCT GTAGACACCGGAGAGTGCAGGCAGCTTCCAAGCCCTCAGAGTCTGAAAGGCAAAATCCTAGTGAAGGGCA AGAAGTTGCCGTATCACCTTGGGGATGATGCAGAGGAAGGAGAAGTGTCCGATGAGGACAGTGCAGATGA GATTGAAGATGAGTGCAAGTTCAAGCTGCATTACAGTAACGGGACCACTGAGCACCAGGTAGAATCTTTC ATACGGAAAAAGCTGGAGTCACTGTTGAAGGAGTCTCAGATTCGAGACAAAGAAGATCCAGACAGTTTCA CAGTGAGGGCGTTACTCAAGGCTACACATGAAGGCTTAAATGCACACCTGAAGCAGAACCTGGATGTAAA GGAAAGTGGAAAGAAGTCCCATGGGCGATCCCTGATGGCCAACTTTGGGAAACACAAGCAGAAAGCTACG AAATCACGTTCTAAATCCTATAGTACTGATGATGAGGATGATAGCCTTCAGAATCCTGGCAAGGAGGGAG GCCAGCTGTACAGGCTGGGCCGCCGAAGGAGAACCATGAAACTCTGCAGAGAGCTCTCGGACTTGGTGGT GTACACAAACTCCGTGGCAGCCCAGGATATTGTTGATGATGGAACCACAGGGAATGTGTTGTCCTTCAGC GAAACAAGAGCCCATCAAGTGGTTCAGCAGAAGTCAGAGCAGTTCATGATCTACAACCAAAAACAGCTCA CAAGGATTTACCCCTCTGCCTACCGCATCGATTCCAGTAACTTCAACCCACTGCCCTACTGGAATGCAGG CTGCCAGCTCGTGGCTCTGAACTACCAGTCTGAAGGGCGAATGATGCAGATCAATCGAGCAAAATTCAAG GCGAATGGCAACTGTGGCTATATTCTCAAGCCCCAGCAAATGTGCAAAGGTACTTTCAACCCTTTCTCTG GTGACCCACTTCCTGCCAACCCCAAAAAACAGCTCATACTGAAAGTGATCAGTGGACAGCAACTCCCCAA ACCTCCTGACTCTATGTTTGGAGACCGAGGCGAGATCATTGACCCTTTCGTTGAAGTTGAAATTATTGGG CTGCCAGTAGACTGTTGCAAGGACCAAACTCGTGTGGTGGATGACAACGGATTTAACCCTGTGTGGGAAG AAACCCTAACTTTTACAGTTCACATGCCAGAAATAGCTTTGGTTCGGTTCCTTGTGTGGGATCATGATCC TATTGGAAGAGACTTTGTCGGACAAAGGACTGTGACCTTCAGTAGCCTCGTGCCCGGCTACCGGCATGTC TACCTGGAAGGACTCACAGAAGCATCTATTTTCGTTCACATAACCATCAATGAGATCTTTGGAAAGTGGA GTCCTTTAATCCTCAACCCCAGTTATACCATATTGCACTTTCTAGGAGCTACAAAGAACAGACAGCTCCA AGGCCTGAAGGGGCTGTTCAATAAGAATCCCAGGCACGCTTCTTCAGAAAACAACTCGCATTATGTTCGG AAGCGATCGATTGGAGATAGGATTCTGCGACGTACAGCTAGTGCTCCAGCCAAAGGCAGGAAAAAGAGCA AAGTGGGCTTCCAAGAAATGGTTGAGATAAAAGATTCTGTCTCTGAGGCTTCAAGAGATCAAGATGGTGT CCTGAGGAGGACCACCCGGAGTCTGCAAGTACGCCCTGTCTCTATGCCTGTTGACAAGAGCCTTCTGGGG GCTTTGTCGCTGCCCATATCTGAAGCAGCAAAAGACACGGATGGAAAAGAAAACTGTCTGGCAGGAGACA AGGATGACAGAAGAAAGGGAGCCGCAACTAGAAAAGACCCACATTTTTCAAATTTCAACAAAAAGTTATC CTCCTCCTCCAGTGCGCTCCTCCACAAAGATGCCAACCAAGGGCCAACTGCCAGTGTATCAAACCCAGAA CAGTGTGGAGGACGAGGTGCAAAGAGTGAGAGGATCAAACCAAATATGACAAATGATTGCCAGGAAAACC ACAATCCCCCGAAATTCCTTTCTCCAAGGAAGCATTTGGCTCTGGATCCTGCAACGAAGGGACTGCAAGA GAGACTGCATGGAATGAAAACCAATGAGAAGGAGCATGCCGAGGGCTTCTTGGGAGAGAAAAGCATGCTG TCCGGAAGCGTTCTTTCTCAAAGTTCTCTGGAGGTAGAGAACCTGGAAGGCAGCAGAGCCAAGGGCAGAG CTGCAACCTCCTTTTCTCTCTCGGACGTCTCCGCGCTCTGTTCTGACATCCCTGATTTACATTCAACTGC CATTCTACAGGACACTGAAATTTCCAATCTCATTGATGACGTGACTTTAACAAATGAGAATCAGTCGGGA AGTTCCATCTCGGCACTGATTGGCCAGTTTGAAGAAAGCAACCACCCGGCTAACGTTACTGTGGTTTCTC ATCTCAGCACCAGTGGGGCATCAGGAAGTGCTCCTTTCCAAACACCCTTCAAGCATGGTCTTTCCCAGGG GAACCAGAAGGCCTCCTTCTTGTGCTCATCTCCTGAGCTGAATAAGCTCTCTAGTGTTGAGACCACCAAA CTGGCAAATAACGCAGTTCCTTGTGGAGTGATTGGTTCTCCCATCTCTACACCAAAGCCAGGTGATGACC CTTCAGATAAGGCCAAGACAAGAGTCATTGAAGGCAACCTGCCTGGGTTCCCTGATGCTTCTCCTGGTCA ATTTCCCAAAAGCCCCACCCATGGAGAGGACCATAGTCAAGTGATGAACAGCCCTGCTCTCTCCACGGAG TTGGCCATAGAAGATATAATTGCAGACCCTGCTCTCTCTATAAATTCTGCAGAAAGCAGTCTTGTGGAAA TTGATGGAGAATCTGAAAACCTATCTCTAACAACCTGTGACTACAGGGAAGAAGCTCCAAGTCAACTTGT TTCTCCACTAAAACTGCAGCAGAGCCAGGAGATGGTGGAACACATTCAGAGGGGCTTGAGGAATGGCTAC TGTAAAGAGACTCTCCTCCCTTCTGAAATATTCAACAATATTCCAGGTGTCAAAAATCACAGTATTTCTC ATCTAACCTATCAGGGTGCTGGCTTTGTGTATAACCATTTCTCAAGTTCAGATGCAAAAACGAACCAAAT CTGTGAGCCCCAGCAGCCTAGGGCTCCAGATATGCATGCCCCTACACCTACACCATCAACACATGCTCCT TTGGCTGCTTTGAAACTGCCAAGCCCATGCAAATCCAAAAGTCTGGGGGACTTAACATCAGAGGACATTG CCTGCAATTTTGAAAGCAAATATCAATGTATTAGTAGGAGCTTTGTGACAAACGGCATTAGGGACAAGAG CGTGACTATGAAGACAAAGTCATTAGAGCCTTTAGACGCCCTGACTGAACAGCTCCGGAAGCTGGTGTCC TTTGACCAAGAAGACAGCTGTCAAGTGCTCTACTCAAAGCAGGATGTCAATCAGTGTCCCAGGGCATTAG TCAGAAAGTTGTCGTCTAGAAGTCAGAGCAGAGTGCGCAACATTGCTAGCCGTGCCAAGGAGAAGCAGGA AGCTGGCAAGCAAAAAGCTATGGCCCAGAGCACCAGAGGAGGAGTGGTCCTTAGAAGTAAACCGCCCGCT CCTGCTCTGGCCGTGAACCGTCACTCCACGGGCTCATACATTGCAAGCTACCTGAGGAACATGAAAGCTG GTGGCCTAGAGGGTCGAGGCATCCCAGAGGGAGCGTGCACGGCCCTTCGCTATGGCTACATGGACCAGTT TTGTTCAGATAATTCAGTTCTGCAGACTGAGCCAAGCAGCGAAGATAAACCCGAAATTTATTTTCTTTTG AGGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212059 representing NM_183191 Red=Cloning site Green=Tags(s) MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIY KVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQ RTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKM MSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIEGFTN FMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDG PDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSS VDTGECRQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESF IRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKQKAT KSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFS ETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQINRAKFK ANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIG LPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHV YLEGLTEASIFVHITINEIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVR KRSIGDRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLG ALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDANQGPTASVSNPE QCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQERLHGMKTNEKEHAEGFLGEKSML SGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVSALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSG SSISALIGQFEESNHPANVTVVSHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTK LANNAVPCGVIGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALSTE LAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQEMVEHIQRGLRNGY CKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKTNQICEPQQPRAPDMHAPTPTPSTHAP LAALKLPSPCKSKSLGDLTSEDIACNFESKYQCISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVS FDQEDSCQVLYSKQDVNQCPRALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPA PALAVNRHSTGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEIYFLL RL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_183191
ORF Size	5046 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_183191.4
RefSeq Size	6266 bp
RefSeq ORF	5049 bp
Locus ID	269437
UniProt ID	Q4KWH5
MW	187.7 kDa
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function]

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