Plch1 (NM_183191) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC042549; PLC-eta-1; PLCeta1; Plcl3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224810 representing NM_183191 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCTTGAAGTGTATAAAAACTTAAGTCCAGAAAAAGTTGAAAGATGCATGAGTGTAATGCAGT CCGGGACACAGATGATCAAACTGAAGCGTGGCACCAAAGGGCTCGTTCGCCTCTTTTACCTGGATGAGCA CCGGACCCGCCTCCGATGGCGACCCTCTCGGAAGAGTGAGAAGGCAAAAATACTTATTGACTCCATCTAC AAAGTCACCGAGGGCAGGCAGTCTGAAATATTCCACAGACAGGCTGAGGGGAACTTTGACCCTAGCTGCT GCTTTACCATCTACCATGGCAACCACATGGAGTCTCTGGACCTCATTACCTCCAACCCAGAGGAGGCACG CACCTGGATCACGGGCCTCAAGTATCTGATGGCTGGCATCAGTGATGAAGACTCCCTTGCCAAGAGGCAG AGGACCCATGACCAATGGGTGAAGCAGACCTTTGAGGAAGCTGATAAGAACGGTGATGGCTTATTGAATA TTGAAGAGATTCACCAGCTGATGCATAAACTAAATGTCAATCTGCCCCGCAGAAAAGTCAGGCAAATGTT TCAGGAAGCAGATACAGATGAGAATCAGGGAACCTTGACATTTGAAGAGTTCTGCGTTTTTTACAAAATG ATGTCATTGAGACGAGACCTCTATTTGCTGCTCTTGAGCTACAGTGACAAGAAAGACCACCTGACTGTGG AGGAGCTGGCTCAGTTTTTGAAAGTGGAACAAAAGATGAGTAATGTGACACTGGACTATTGTCTTGACAT CATAATGAAGTTTGAAGTTTCTGAAGAAAACAAAGTGAAAAACGTCCTTGGTATAGAAGGCTTCACGAAC TTCATGCGTAGCCCTGCCTGTGACGTATTTAACCCGTTGCACCATGAAGTGTACCAAGACATGGATCAGC CCCTGTGCAACTACTACATTGCTTCATCTCACAACACATACCTGACTGGGGATCAGCTCCTTTCTCAATC TAAAGTGGATATGTATGCACGGGTGCTACAAGAGGGTTGTAGATGCGTGGAAGTTGACTGTTGGGATGGC CCAGATGGAGAGCCAGTGGTCCACCATGGTTATACTCTCACTTCAAAAATTCTCTTCAGAGATGTTGTGG AGACCATCAACAAGCATGCATTCGTGAAGAATGAGTTCCCGGTTATCCTGTCCATTGAGAACCACTGCAG CATTCAGCAGCAGAGGAAGATTGCTCAGTACCTGAAAGGTATATTGCAGGACAAACTGGACCTGTCTTCT GTAGACACCGGAGAGTGCAGGCAGCTTCCAAGCCCTCAGAGTCTGAAAGGCAAAATCCTAGTGAAGGGCA AGAAGTTGCCGTATCACCTTGGGGATGATGCAGAGGAAGGAGAAGTGTCCGATGAGGACAGTGCAGATGA GATTGAAGATGAGTGCAAGTTCAAGCTGCATTACAGTAACGGGACCACTGAGCACCAGGTAGAATCTTTC ATACGGAAAAAGCTGGAGTCACTGTTGAAGGAGTCTCAGATTCGAGACAAAGAAGATCCAGACAGTTTCA CAGTGAGGGCGTTACTCAAGGCTACACATGAAGGCTTAAATGCACACCTGAAGCAGAACCTGGATGTAAA GGAAAGTGGAAAGAAGTCCCATGGGCGATCCCTGATGGCCAACTTTGGGAAACACAAGCAGAAAGCTACG AAATCACGTTCTAAATCCTATAGTACTGATGATGAGGATGATAGCCTTCAGAATCCTGGCAAGGAGGGAG GCCAGCTGTACAGGCTGGGCCGCCGAAGGAGAACCATGAAACTCTGCAGAGAGCTCTCGGACTTGGTGGT GTACACAAACTCCGTGGCAGCCCAGGATATTGTTGATGATGGAACCACAGGGAATGTGTTGTCCTTCAGC GAAACAAGAGCCCATCAAGTGGTTCAGCAGAAGTCAGAGCAGTTCATGATCTACAACCAAAAACAGCTCA CAAGGATTTACCCCTCTGCCTACCGCATCGATTCCAGTAACTTCAACCCACTGCCCTACTGGAATGCAGG CTGCCAGCTCGTGGCTCTGAACTACCAGTCTGAAGGGCGAATGATGCAGATCAATCGAGCAAAATTCAAG GCGAATGGCAACTGTGGCTATATTCTCAAGCCCCAGCAAATGTGCAAAGGTACTTTCAACCCTTTCTCTG GTGACCCACTTCCTGCCAACCCCAAAAAACAGCTCATACTGAAAGTGATCAGTGGACAGCAACTCCCCAA ACCTCCTGACTCTATGTTTGGAGACCGAGGCGAGATCATTGACCCTTTCGTTGAAGTTGAAATTATTGGG CTGCCAGTAGACTGTTGCAAGGACCAAACTCGTGTGGTGGATGACAACGGATTTAACCCTGTGTGGGAAG AAACCCTAACTTTTACAGTTCACATGCCAGAAATAGCTTTGGTTCGGTTCCTTGTGTGGGATCATGATCC TATTGGAAGAGACTTTGTCGGACAAAGGACTGTGACCTTCAGTAGCCTCGTGCCCGGCTACCGGCATGTC TACCTGGAAGGACTCACAGAAGCATCTATTTTCGTTCACATAACCATCAATGAGATCTTTGGAAAGTGGA GTCCTTTAATCCTCAACCCCAGTTATACCATATTGCACTTTCTAGGAGCTACAAAGAACAGACAGCTCCA AGGCCTGAAGGGGCTGTTCAATAAGAATCCCAGGCACGCTTCTTCAGAAAACAACTCGCATTATGTTCGG AAGCGATCGATTGGAGATAGGATTCTGCGACGTACAGCTAGTGCTCCAGCCAAAGGCAGGAAAAAGAGCA AAGTGGGCTTCCAAGAAATGGTTGAGATAAAAGATTCTGTCTCTGAGGCTTCAAGAGATCAAGATGGTGT CCTGAGGAGGACCACCCGGAGTCTGCAAGTACGCCCTGTCTCTATGCCTGTTGACAAGAGCCTTCTGGGG GCTTTGTCGCTGCCCATATCTGAAGCAGCAAAAGACACGGATGGAAAAGAAAACTGTCTGGCAGGAGACA AGGATGACAGAAGAAAGGGAGCCGCAACTAGAAAAGACCCACATTTTTCAAATTTCAACAAAAAGTTATC CTCCTCCTCCAGTGCGCTCCTCCACAAAGATGCCAACCAAGGGCCAACTGCCAGTGTATCAAACCCAGAA CAGTGTGGAGGACGAGGTGCAAAGAGTGAGAGGATCAAACCAAATATGACAAATGATTGCCAGGAAAACC ACAATCCCCCGAAATTCCTTTCTCCAAGGAAGCATTTGGCTCTGGATCCTGCAACGAAGGGACTGCAAGA GAGACTGCATGGAATGAAAACCAATGAGAAGGAGCATGCCGAGGGCTTCTTGGGAGAGAAAAGCATGCTG TCCGGAAGCGTTCTTTCTCAAAGTTCTCTGGAGGTAGAGAACCTGGAAGGCAGCAGAGCCAAGGGCAGAG CTGCAACCTCCTTTTCTCTCTCGGACGTCTCCGCGCTCTGTTCTGACATCCCTGATTTACATTCAACTGC CATTCTACAGGACACTGAAATTTCCAATCTCATTGATGACGTGACTTTAACAAATGAGAATCAGTCGGGA AGTTCCATCTCGGCACTGATTGGCCAGTTTGAAGAAAGCAACCACCCGGCTAACGTTACTGTGGTTTCTC ATCTCAGCACCAGTGGGGCATCAGGAAGTGCTCCTTTCCAAACACCCTTCAAGCATGGTCTTTCCCAGGG GAACCAGAAGGCCTCCTTCTTGTGCTCATCTCCTGAGCTGAATAAGCTCTCTAGTGTTGAGACCACCAAA CTGGCAAATAACGCAGTTCCTTGTGGAGTGATTGGTTCTCCCATCTCTACACCAAAGCCAGGTGATGACC CTTCAGATAAGGCCAAGACAAGAGTCATTGAAGGCAACCTGCCTGGGTTCCCTGATGCTTCTCCTGGTCA ATTTCCCAAAAGCCCCACCCATGGAGAGGACCATAGTCAAGTGATGAACAGCCCTGCTCTCTCCACGGAG TTGGCCATAGAAGATATAATTGCAGACCCTGCTCTCTCTATAAATTCTGCAGAAAGCAGTCTTGTGGAAA TTGATGGAGAATCTGAAAACCTATCTCTAACAACCTGTGACTACAGGGAAGAAGCTCCAAGTCAACTTGT TTCTCCACTAAAACTGCAGCAGAGCCAGGAGATGGTGGAACACATTCAGAGGGGCTTGAGGAATGGCTAC TGTAAAGAGACTCTCCTCCCTTCTGAAATATTCAACAATATTCCAGGTGTCAAAAATCACAGTATTTCTC ATCTAACCTATCAGGGTGCTGGCTTTGTGTATAACCATTTCTCAAGTTCAGATGCAAAAACGAACCAAAT CTGTGAGCCCCAGCAGCCTAGGGCTCCAGATATGCATGCCCCTACACCTACACCATCAACACATGCTCCT TTGGCTGCTTTGAAACTGCCAAGCCCATGCAAATCCAAAAGTCTGGGGGACTTAACATCAGAGGACATTG CCTGCAATTTTGAAAGCAAATATCAATGTATTAGTAGGAGCTTTGTGACAAACGGCATTAGGGACAAGAG CGTGACTATGAAGACAAAGTCATTAGAGCCTTTAGACGCCCTGACTGAACAGCTCCGGAAGCTGGTGTCC TTTGACCAAGAAGACAGCTGTCAAGTGCTCTACTCAAAGCAGGATGTCAATCAGTGTCCCAGGGCATTAG TCAGAAAGTTGTCGTCTAGAAGTCAGAGCAGAGTGCGCAACATTGCTAGCCGTGCCAAGGAGAAGCAGGA AGCTGGCAAGCAAAAAGCTATGGCCCAGAGCACCAGAGGAGGAGTGGTCCTTAGAAGTAAACCGCCCGCT CCTGCTCTGGCCGTGAACCGTCACTCCACGGGCTCATACATTGCAAGCTACCTGAGGAACATGAAAGCTG GTGGCCTAGAGGGTCGAGGCATCCCAGAGGGAGCGTGCACGGCCCTTCGCTATGGCTACATGGACCAGTT TTGTTCAGATAATTCAGTTCTGCAGACTGAGCCAAGCAGCGAAGATAAACCCGAAATTTATTTTCTTTTG AGGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_183191
Insert Size	5049 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_183191.3, NP_899014.2
RefSeq Size	6266 bp
RefSeq ORF	5049 bp
Locus ID	269437
UniProt ID	Q4KWH5
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1, also known as PLC-eta1c) encodes the longest isoform (1, also known as phospholipase C-eta1c). CCDS Note: The coding region has been updated to represent an alternate splice pattern that is more supported by the available conservation, transcript and protein data. The update changes the protein length from 1640 aa to 1682 aa.

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