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Dnmt1 (BC053047) Mouse Tagged ORF Clone
CAT＃: MR212057
- TrueORF®
Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259)
ORF Plasmid: tGFP
"BC053047" in other vectors (4)
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CNY 11,136.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dnmt, MTase, Dnmt1o |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212057 representing BC053047
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCGCGAACAGCTCCAGCCCGAGTGCCTGCGCTTGCCTCCCCGGCAGGCTCGCTCCCGGACCATG TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAGGGAGAAATTAAA CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGTTGTGTGACTTGGAAACCAAATTACATAAA GAGGAATTATCTGAGGAAGGCTACCTGGCTAAAGTCAAGTCCCTCTTAAATAAGGATTTGTCCTTGGAGA ACGGAACACACACTCTCACTCAAAAAGCCAACGGTTGTCCCGCCAACGGGAGCCGGCCAACCTGGAGAGC AGAAATGGCAGACTCAAATAGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGAAGCAAGTCG GACAGTGACACCCTTTGTAAGGACACGAGACATACTGCAGTTGAAACTTCACCTAGTTCCGTGGCTACGA GGAGAACCACCAGGCAGACCACCATCACGGCTCACTTCACGAAGGGCCCCACTAAACGGAAACCCAAGGA AGAGTCGGAAGAGGGGAACTCGGCTGAGTCGGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTT GTAGACACAGAGAGTGGTGCTGCAGCTGCTGTGGAGAAACTGGAAGAGGTAACAGCGGGAACCCAGCTGG GTCCGGAAGAGCCATGTGAACAGGAAGATGACAACAGGAGTCTTCGACGTCACACCAGAGAGCTATCATT GAGGCGGAAATCAAAGGAGGATCCAGACAGAGAAGCAAGACCGGAAACTCACTTGGACGAGGACGAGGAC GGAAAAAAGGATAAAAGAAGTTCCAGACCCAGGAGCCAGCCCAGAGATCCAGCTGCCAAACGGAGACCCA AGGAAGCAGAGCCAGAGCAGGTAGCTCCAGAGACTCCCGAGGACAGAGACGAGGATGAGAGGGAGGAGAA GAGACGAAAAACGACACGTAAAAAACTGGAGTCACACACCGTTCCCGTTCAGAGCAGATCGGAGAGAAAA GCCGCTCAAAGCAAAAGTGTGATCCCGAAGATCAACTCACCAAAGTGCCCCGAGTGTGGCCAGCACCTAG ACGACCCTAACCTGAAGTACCAGCAGCACCCTGAGGATGCTGTGGATGAACCCCAGATGTTGACCAGTGA GAAACTGTCCATCTACGACTCCACCTCGACCTGGTTTGATACTTATGAAGATTCTCCCATGCATAGGTTC ACTTCCTTCAGTGTGTACTGCAGTCGCGGGCACCTGTGTCCTGTCGACACCGGTCTCATTGAGAAGAATG TAGAGCTCTACTTTTCTGGGTGTGCCAAAGCAATTCATGACGAGAATCCATCTATGGAAGGTGGTATTAA TGGCAAAAACCTCGGGCCAATCAATCAGTGGTGGCTCAGTGGCTTTGATGGTGGCGAGAAGGTGCTCATT GGCTTCTCCACTGCATTTGCTGAATACATTTTGATGGAGCCCAGCAAAGAGTATGAGCCAATATTTGGGC TGATGCAGGAGAAAATTTACATCAGCAAGATTGTTGTTGAGTTCCTGCAAAACAATCCTGATGCTGTATA TGAAGACCTGATCAATAAGATTGAGACCACTGTTCCTCCTTCTACCATTAATGTGAACCGGTTCACAGAG GACTCCCTCTTACGCCACGCCCAGTTTGTAGTGAGCCAGGTAGAGAGTTACGACGAAGCCAAGGACGATG ATGAGACCCCCATCTTCTTGTCTCCCTGTATGAGAGCCCTGATCCATTTGGCTGGTGTCTCCCTGGGACA GAGGCGAGCAACAAGGCGCGTCATGGGTGCTACCAAGGAGAAGGACAAAGCACCCACGAAAGCCACCACC ACCAAGCTGGTCTATCAGATCTTTGACACTTTCTTCTCAGAGCAGATTGAGAAGTATGATAAGGAGGACA AGGAGAATGCCATGAAGCGCCGCCGCTGTGGTGTCTGTGAGGTCTGTCAGCAGCCTGAGTGTGGGAAGTG CAAGGCGTGCAAAGATATGGTGAAGTTTGGTGGCACTGGACGGAGTAAGCAGGCTTGCCTCAAGAGGAGG TGTCCTAACTTGGCGGTGAAGGAGGCAGACGACGATGAAGAGGCTGATGATGATGTGTCAGAGATGCCAT CACCCAAAAAGCTGCATCAGGGGAAGAAGAAGAAGCAGAACAAGGACCGCATCTCCTGGCTTGGGCAGCC TATGAAGATTGAAGAGAATAGAACTTACTATCAGAAGGTGAGCATCGATGAGGAGATGCTAGAGGTGGGC GACTGCGTCTCGGTCATTCCAGATGATTCCTCCAAACCACTCTATCTAGCCAGGGTCACAGCTCTGTGGG AAGACAAAAATGGTCAGATGATGTTCCATGCGCACTGGTTCTGCGCTGGGACAGACACAGTCCTGGGAGC CACCTCCGACCCCCTGGAACTGTTCCTGGTGGGCGAGTGCGAAAACATGCAGCTTTCCTACATCCACAGC AAGGTCAAGGTCATCTACAAAGCCCCTTCTGAAAACTGGGCCATGGAGGGAGGCACAGACCCTGAGACCA CACTGCCTGGGGCTGAGGATGGCAAGACTTACTTCTTCCAGCTCTGGTACAACCAGGAGTACGCAAGGTT TGAATCCCCACCCAAGACCCAGCCGACCGAGGACAACAAGCACAAGTTCTGCCTATCTTGTATCCGGCTG GCTGAGCTGAGACAAAAAGAAATGCCCAAGGTCCTGGAACAAATTGAGGAGGTGGATGGCCGGGTCTACT GCAGTTCCATCACCAAGAATGGTGTTGTCTACCGACTGGGTGACAGTGTGTACCTTCCTCCCGAGGCCTT TACTTTCAACATCAAAGTGGCTAGCCCCGTGAAACGCCCAAAGAAGGATCCTGTGAACGAGACCCTGTAC CCTGAGCACTACCGCAAGTATTCTGACTACATCAAGGGGAGCAACCTGGATGCTCCAGAGCCCTATCGCA TCGGTCGGATAAAAGAGATCCACTGTGGCAAGAAGAAAGGCAAGGTCAACGAGGCAGACATCAAGCTGAG GCTCTACAAGTTCTACAGGCCTGAGAATACCCACAGGTCCTACAACGGATCCTATCACACTGACATCAAC ATGCTTTACTGGAGCGACGAGGAAGCTGTGGTGAACTTCAGCGACGTGCAGGGCCGCTGTACCGTGGAGT ACGGGGAAGACCTACTTGAGAGCATCCAGGATTATTCACAAGGGGGCCCTGACCGCTTCTACTTCCTCGA GGCCTACAATTCAAAGACCAAGAACTTTGAAGACCCACCAAACCATGCCCGCAGCCCTGGGAACAAAGGG AAAGGGAAGGGGAAAGGGAAGGGGAAGGGGAAGCATCAGGTGTCAGAGCCCAAAGAGCCTGAGGCAGCCA TCAAACTGCCCAAGCTCCGGACCCTGGATGTGTTTTCCGGCTGTGGAGGGTTATCGGAAGGATTCCACCA AGCAGGCATCTCGGAAACGCTGTGGGCCATCGAGATGTGGGACCCGGCAGCCCAGGCATTTCGGCTGAAC AACCCCGGCACCACAGTGTTCACAGAGGACTGCAACGTGCTTCTTAAGCTGGTCATGGCTGGGGAGGTGA CCAACTCTCTGGGCCAAAGGCTGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCACCCTGCCA GGGCTTCAGTGGCATGAACCGCTTCAACTCCCGCACTTACTCCAAGTTCAAAAACTCCCTAGTGGTCTCC TTCCTCAGCTACTGTGACTACTACCGGCCTCGGTTCTTCCTTCTGGAGAACGTCAGGAACTTCGTGTCCT ACAGACGCTCCATGGTGCTGAAGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTTGG TGTGCTCCAGGCTGGACAGTATGGCGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCA GGAGAAAAGCTGCCTCTGTTCCCAGAGCCTCTGCATGTGTTTGCGCCCCGTGCCTGCCAGCTGAGCGTTG TGGTGGATGACAAGAAGTTTGTTAGCAACATAACGAGGCTGAGCTCGGGGCCCTTCCGAACCATCACCGT GCGAGACACCATGTCTGACCTCCCCGAGATCCAGAATGGAGCCTCGAATTCTGAGATCCCCTACAATGGA GAGCCACTGTCCTGGTTCCAGAGGCAGCTGCGAGGATCACACTACCAGCCCATCCTCAGGGACCATATCT GCAAGGACATGAGCCCACTGGTGGCTGCCCGCATGCGGCACATCCCACTGTTCCCAGGATCAGATTGGCG TGACCTGCCCAACATACAGGTGCGGCTGGGAGATGGCGTCATAGCCCATAAGCTACAGTACACCTTTCAT GATGTGAAAAATGGCTACAGCAGCACCGGTGCCCTGCGTGGAGTCTGTTCCTGTGCAGAAGGCAAGGCCT GCGACCCTGAGTCCAGGCAATTCAGCACCCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAA CCACTGGGCTGGCCTCTACGGGCGTCTGGAGTGGGATGGCTTCTTCAGCACCACTGTCACCAACCCTGAG CCCATGGGCAAGCAGGGTCGGGTGCTCCACCCGGAGCAGCACCGGGTCGTGAGTGTTCGGGAATGTGCCC GCTCCCAGGGCTTTCCAGATAGCTACCGGTTCTTCGGCAACATCCTGGACAGACACCGGCAGGTGGGTAA TGCTGTGCCACCACCCCTGGCCAAAGCCATTGGCCTGGAGATTAAGCTCTGCCTGCTGTCCAGTGCTCGG GAGAGCGCATCAGCTGCAGTTAAAGCAAAAGAGGAGGCTGCTACCAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212057 representing BC053047
Red=Cloning site Green=Tags(s) MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHK EELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKS DSDTLCKDTRHTAVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRV VDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDED GKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERK AAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRF TSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLI GFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTE DSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATT TKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRR CPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVG DCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHS KVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRL AELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLY PEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDIN MLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKG KGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLN NPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVS FLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAP GEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNG EPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFH DVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPE PMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSAR ESASAAVKAKEEAATKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053047 |
| ORF Size | 4881 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC053047.1 |
| RefSeq Size | 5249 bp |
| RefSeq ORF | 4883 bp |
| Locus ID | 13433 |
| MW | 184 kDa |
| Gene Summary | This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224567 | Dnmt1 (untagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259), (10ug) |
CNY 19,190.00 |
|
| MG212057 | Dnmt1 (tGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259) |
CNY 12,736.00 |
|
| MR212057L3 | Lenti ORF clone of Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259) |
CNY 11,210.00 |
|
| MR212057L4 | Lenti ORF clone of Dnmt1 (mGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259) |
CNY 11,210.00 |
