Slit1 (NM_015748) Mouse Tagged ORF Clone
CAT#: MR212022
- TrueORF®
Slit1 (Myc-DDK-tagged) - Mouse slit homolog 1 (Drosophila) (Slit1)
ORF Plasmid: tGFP
"NM_015748" in other vectors (4)
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CNY 15,712.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | mKIAA0813; Slil1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR212022 representing NM_015748
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTAACGCCCCAGCGCGGGTCCTCTTCGGGGTTGAGCCGACCGGAGCTGTGGCTGCTGCTGTGGG CAGCCGCGTGGCGCCTGGGGGCCACGGCGTGCCCCGCCCTCTGCACCTGCACCGGCACTACGGTGGACTG CCACGGCACGGGTTTGCAGGCCATCCCCAAGAACATCCCACGGAACACCGAGCGCCTGGAACTCAACGGC AACAACATCACTCGGATCCACAAGAATGACTTTGCTGGCCTCAAGCAGCTTCGAGTGTTGCAGCTGATGG AGAACCAGATCGGAGCTGTGGAACGCGGAGCTTTTGATGACATGAAGGAGCTAGAGCGCCTACGGCTGAA CCGCAACCAGCTACAAGTGTTGCCTGAACTACTGTTCCAGAACAACCAGGCTCTATCCAGACTGGACCTG AGTGAGAATTTTCTCCAGGCTGTGCCCAGGAAGGCTTTTCGGGGAGCCACAGACCTCAAAAATCTACAGC TGGACAAGAACCGGATCAGCTGCATCGAGGAAGGGGCCTTCCGTGCCCTACGGGGACTGGAGGTTCTGAC CCTGAACAACAACAACATCACCACCATCCCTGTGTCCAGTTTCAACCATATGCCCAAGCTTCGGACCTTC CGGCTGCACTCCAACCACCTGTTCTGTGACTGCCACCTGGCCTGGCTCTCTCAGTGGCTGAGACAGAGGC CCACCATCGGACTCTTCACCCAGTGCTCTGGGCCCGCCAGCCTTCGGGGCCTCAACGTGGCAGAGGTGCA AAAGGGCGAGTTCAGCTGCTCAGGCCAGGGGGAGGCTGCGGGAGCACCTGCCTGCACCCTCTCGTCCGGT TCCTGCCCAGCCATGTGCAGCTGTAGCAGCGGCATCGTGGACTGCCGTGGCAAGGGCCTCACTGCCATCC CTGCTAACCTTCCAGAGACCATGACAGAGATCCGCCTGGAGTTGAACGGTATCAAATCCATCCCACCCGG AGCCTTCTCTCCCTACAGAAAGCTGCGCAGGATAGACCTGAGCAACAACCAGATTGCAGAGATTGCACCC GACGCCTTCCAAGGCCTGCGCTCCTTGAATTCCCTGGTCCTCTATGGCAACAAGATTACAGACCTGCCCC GAGGTGTATTTGGAGGCCTGTACACACTCCAGCTGCTGCTTCTAAATGCCAACAAGATCAACTGCATCCG GCCTGATGCCTTCCAGGACCTGCAGAACCTCTCTCTGCTCTCCCTGTATGACAACAAGATCCAGAGTCTC GCCAAGGGCACCTTCACCTCCCTACGGGCCATCCAGACACTGCATCTGGCCCAGAATCCTTTCATCTGTG ACTGTAACCTCAAGTGGCTGGCAGACTTCTTACGTACCAACCCCATCGAGACAACTGGGGCCCGATGTGC CAGCCCCCGGCGCCTCGCCAACAAGCGTATTGGGCAGATCAAAAGCAAGAAGTTTCGGTGCTCAGCCAAA GAGCAGTACTTCATTCCAGGGACAGAGGACTACCACCTGAACAGCGAGTGTACCAGCGACGTGGCCTGCC CACACAAGTGCCGTTGTGAGGCCAGTGTGGTGGAGTGCTCCAGCCTGAAACTCTCTAAGATCCCTGAGCG AATCCCCCAGTCCACGACTGAGCTACGACTGAACAACAATGAGATTTCTATCCTAGAGGCCACTGGGTTG TTTAAGAAGCTCTCACATCTGAAGAAAATAAACCTGAGCAATAACAAGGTGTCCGAGATCGAAGACGGGA CGTTTGAGGGTGCCGCCTCTGTCAGTGAGCTGCACCTGACTGCTAACCAGCTGGAGTCCATTAGAAGTGG CATGTTCCGGGGTCTAGATGGCTTGCGGACCCTGATGCTGAGGAACAACCGTATCAGCTGTATCCACAAC GACAGCTTCACCGGGCTGCGCAACGTGCGGCTCCTGTCTCTCTATGACAACCACATCACCACCATCTCCC CTGGAGCCTTTGACACTCTCCAGGCCCTGTCCACACTAAACCTCCTGGCCAACCCGTTCAACTGCAACTG CCATCTGTCATGGCTGGGCGACTGGCTCCGGAAGAGGAAGATTGTGACGGGGAACCCTCGCTGTCAGAAC CCAGACTTCCTGCGGCAGATTCCTCTGCAGGATGTGGCCTTCCCTGACTTCAGGTGTGAGGAAGGCCAGG AGGAGGTGGGCTGCCTGCCCCGCCCACAGTGCCCCCAGGAGTGTGCCTGCCTGGACACAGTGGTCCGCTG CAGCAACAAGCACCTCCAGGCTCTGCCCAAGGGCATCCCCAAGAATGTCACAGAACTCTATTTGGATGGG AACCAGTTCACACTCGTTCCGGGACAGCTGTCTACCTTCAAGTATCTGCAGCTCGTGGACTTGAGCAACA ACAAGATCAGTTCCTTGAGCAATTCTTCCTTCACCAACATGAGCCAGCTGACCACGCTGATCCTCAGCTA CAATGCCCTCCAGTGCATCCCTCCTCTGGCCTTTCAAGGACTTCGTTCGCTGCGCCTGCTGTCTTTGCAT GGCAATGATGTCTCCACCCTCCAAGAGGGCATCTTTGCAGACGTGACGTCTCTGTCTCACCTGGCCATTG GTGCCAACCCCCTGTACTGCGACTGCCGCCTCCGGTGGCTCTCCAGTTGGGTGAAGACTGGCTACAAGGA ACCTGGCATTGCCCGCTGTGCTGGACCCCCAGAGATGGAGGGCAAACTGTTACTCACCACACCTGCCAAG AAGTTTGAATGCCAAGGTCCCCCCAGCCTGGCAGTTCAGGCCAAGTGTGATCCCTGCTTGTCCAGCCCAT GCCAGAACCAGGGCACCTGCCACAATGACCCTCTTGAGGTATACAGGTGTACCTGCCCCAGTGGCTACAA GGGCCGACACTGTGAGGTGTCTCTGGACGGCTGTTCCAGCAACCCATGTGGAAATGGGGGGACCTGCCAT GCCCAGGAGGGCGAGGATGCTGGCTTCACGTGCTCCTGTCCCTCCGGCTTTGAAGGGCCGACCTGTGGAG TGGACACAGATGACTGTGTTAAGCATGCCTGTGTCAACGGGGGTGTCTGTGTAGATGGCGTGGGGAATTA CACATGTCAGTGCCCCCTGCAGTACACAGGAAGGGCCTGTGAGCAGCTGGTGGACTTCTGCTCCCCGGAT ATGAACCCGTGTCAGCATGAGGCCCAATGTGTTGGCACCCCGGATGGGCCCAGGTGTGAGTGCATGCTAG GTTACACGGGTGACAACTGCAGCGAAAATCAGGATGACTGCAAGGACCACAAGTGTCAGAACGGGGCCCA GTGTGTGGATGAAGTCAACAGCTACGCCTGCCTCTGTGTTGAGGGCTACAGTGGACAGCTCTGTGAGATC CCACCTGCCCCCAGGAGCTCCTGTGAGGGGACGGAGTGCCAGAATGGAGCCAACTGCGTAGACCAGGGAA GCCGGCCCGTATGCCAGTGCCTACCAGGCTTTGGTGGCCCTGAATGTGAGAAATTGCTCAGTGTCAACTT TGTGGACCGGGACACTTATCTGCAGTTCACTGACCTGCAGAACTGGCCTCGGGCCAACATCACTCTTCAG GTCTCCACAGCAGAGGACAATGGGATTCTCCTATATAATGGGGACAATGACCACATTGCAGTTGAGCTGT ACCAGGGCCATGTCCGTGTTAGTTATGACCCAGGCAGCTACCCCAGCTCTGCTATCTACAGCGCTGAGAC GATCAACGATGGGCAGTTCCACACCGTTGAGCTGGTCACCTTCGACCAGATGGTGAACCTCTCCATCGAT GGCGGCAGCCCCATGACCATGGACAACTTTGGCAAGCACTACACCCTCAACAGCGAGGCCCCCCTCTATG TGGGAGGGATGCCTGTGGACGTGAACTCAGCTGCCTTCCGCCTGTGGCAGATCCTCAATGGTACCAGCTT CCACGGTTGCATCCGGAACCTGTACATCAACAATGAACTGCAGGACTTCACCAAGACACAGATGAAGCCA GGCGTGGTGCCAGGCTGTGAGCCGTGCCGCAAACTCTACTGTCTACATGGCATTTGCCAGCCCAACGCCA CCCCAGGGCCTGTGTGCCACTGTGAGGCTGGCTGGGGGGGCCTGCACTGTGACCAGCCAGTGGATGGCCC CTGCCATGGCCACAAGTGTGTCCATGGGAAATGCGTGCCGCTCGACGCTCTTGCCTACAGCTGCCAGTGC CAGGATGGGTACTCGGGGGCTCTGTGCAACCAGGTCGGGGCTGTGGCAGAGCCCTGTGGGGGCTTGCAGT GCCTGCATGGTCACTGCCAGGCCTCTGCCACCAAAGGGGCACACTGTGTGTGCAGCCCAGGCTTTTCAGG CGAGCTGTGTGAGCAAGAGTCCGAGTGCCGGGGGGACCCTGTCCGGGACTTTCACCGGGTCCAGAGGGGC TATGCCATCTGCCAGACCACACGCCCGCTGTCGTGGGTGGAATGCCGGGGCGCGTGCCCGGGCCAGGGCT GCTGCCAGGGCCTGCGGCTGAAGCGGAGGAAGCTCACCTTCGAGTGCAGCGATGGGACTTCGTTTGCTGA GGAGGTGGAGAAGCCCACCAAGTGTGGCTGTGCCCAGTGCGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212022 representing NM_015748
Red=Cloning site Green=Tags(s) MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNG NNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDL SENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTF RLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQGEAAGAPACTLSSG SCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAP DAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSL AKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAK EQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGL FKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHN DSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQN PDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDG NQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLH GNDVSTLQEGIFADVTSLSHLAIGANPLYCDCRLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAK KFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRHCEVSLDGCSSNPCGNGGTCH AQEGEDAGFTCSCPSGFEGPTCGVDTDDCVKHACVNGGVCVDGVGNYTCQCPLQYTGRACEQLVDFCSPD MNPCQHEAQCVGTPDGPRCECMLGYTGDNCSENQDDCKDHKCQNGAQCVDEVNSYACLCVEGYSGQLCEI PPAPRSSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQ VSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSID GGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKP GVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQC QDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATKGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRG YAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAQCA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015748 |
ORF Size | 4593 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015748.3, NP_056563.2 |
RefSeq Size | 5300 bp |
RefSeq ORF | 4596 bp |
Locus ID | 20562 |
UniProt ID | Q80TR4 |
MW | 167.9 kDa |
Gene Summary | Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224651 | Slit1 (untagged) - Mouse slit homolog 1 (Drosophila) (Slit1), (10ug) |
CNY 19,760.00 |
|
MG212022 | Slit1 (tGFP-tagged) - Mouse slit homolog 1 (Drosophila) (Slit1) |
CNY 16,820.00 |
|
MR212022L3 | Lenti ORF clone of Slit1 (Myc-DDK-tagged) - Mouse slit homolog 1 (Drosophila) (Slit1) |
CNY 17,200.00 |
|
MR212022L4 | Lenti ORF clone of Slit1 (mGFP-tagged) - Mouse slit homolog 1 (Drosophila) (Slit1) |
CNY 17,200.00 |