Synj2 (BC060214) Mouse Tagged ORF Clone

CAT#: MR211941

  • TrueORF®

Synj2 (Myc-DDK-tagged) - Mouse synaptojanin 2 (cDNA clone MGC:77971 IMAGE:6838657)

ORF Plasmid: DDK tGFP



  "BC060214" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 13,490.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI481647; mKIAA0348; SJ2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211941 representing BC060214
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGTGGGCAGAATTCCAGATGCAGAGATCTACAAAATCACTGCCACTGAGTTGTACCCCCTGCAGG
AAGAGGCCAAGGAAGAGGACCGCCTGCCCACCTTAAAGAAAATCCTGAGCTCAGGGGTGTTCTATTTCGC
ATGGCCCAATGATGGCGCCTGCTTCGATCTGACCATCAGGGCTCAGAAACAGGGTGATGACGGCTCTGAA
TGGGGGACCTCTTTCTTCTGGAACCAGCTATTGCATGTGCCTCTGCGGCAGCACCAGGTGAACTGTCATA
ACTGGTTGCTGAAAGTCATCTGTGGGGTGGTGACCATCCGCACAGTATATGCCTCCCACAAGCAGGCCAA
GGCCTGTCTCATCTCTCGCATCAGCTGTGAACGCGCAGGTGCTCGCTTCCTCACCCGTGGTGTGAACGAT
GATGGCCACGTGTCCAACTTTGTGGAGACAGAGCAGACGATTTACATGGATGATGGAGTATCGTCCTTTG
TCCAGATCCGAGGCTCCGTTCCGCTGTTCTGGGAGCAACCAGGACTTCAGGTTGGCTCCCATCATCTGAG
ACTGCACAGAGGCCTAGAGGCCAACGCTCCTGCTTTTGAAAGGCACATGGTGCTTCTGAAGGAGCAATAC
GGTAAGCAGGTGGTTGTGAACCTGCTGGGTAGCAGAGGCGGTGAAGAGGTGCTCAACAGAGCCTTCAAGA
AGTTGCTCTGGGCTTCTTGCCACGCGGGTGACACACCTATGATAAATTTTGACTTCCATCAGTTTGCCAA
AGGTAGGAAGCTAGAGAAATTGGAGAACCTGTTGAGACCTCAGTTACAGCTACACTGGGAAGACTTCGGC
GTGTTTGCGAAGGGCGAGAATGTAAGTCCACGGTTTCAGAAAGGCACTCTGCGGATGAACTGTCTCGACT
GTCTGGATAGAACCAACACTGTGCAGTGCTTCATTGCTCTTGAGGTCCTTCATCTGCAGCTTGAGAGCTT
GGGGCTAAATTCAAAGCCCATCATTGACCGTTTTGTGGAGTCCTTCAAAGCCATGTGGTCTCTGAATGGG
CACAGCCTGAGCAAGGTGTTCACAGGGAGCAGGGCCCTGGAAGGAAAGGCCAAGGTGGGAAAGCTGAAGG
ATGGGGCCCGATCCATGTCTCGCACCATCCAGTCCAACTTCTTCGACGGGGTGAAGCAGGAGGCCATCAA
GCTACTGCTAGTCGGAGATGTCTACAATGAAGAGTCTACAGACAAAGGACGGATGCTGCTGGACAACACG
GCCCTTCTGGCGACCCCCAGGATCTTGAAGGCCATGACAGAACGCCAGTCGGAATTCACGAATTTCAAGC
GCATCCAGATTGCTGTGGGGACCTGGAATGTGAACGGAGGAAAGCAATTCCGTAGCAATCTCCTGGGGAC
GGCTGAGCTCACGGACTGGCTCCTAGATGCTCCTCAGCTGTCAGGAGCAGTGGACTCCCAGGATGATGGC
AGTCCTGCTGACGTATTTGCCATCGGGTTTGAGGAGATGGTGGAACTGAGTGCGGGAAATATTGTCAATG
CCAGCACCACCAACAGGAAGATGTGGGGCGAGCAGCTTCAGAAAGCCATCTCCCGCTCCCATCGGTACAT
CCTCTTGACCTCCGCACAGCTGGTGGGCGTCTGTCTTTACATCTTTGTACGTCCGTACCACGTCCCGTTC
ATCAGAGACGTGGCCATCGACACCGTGAAGACCGGCATGGGGGGAAAGGCGGGGAATAAGGGTGCTGTGG
GCATCCGCTTCCAGCTCCACAGCACCAGTTTCTGCTTCGTCTGTAGCCACCTGACGGCTGGGCAGTCTCA
GGTGAAGGAGAGGAATGAAGACTACCGGGAGATCACGCACAAACTCTCCTTCCCTTCGGGGAGAAACATA
TTTTCACATGATTACGTGTTTTGGTGTGGCGATTTCAACTACCGTATTGATCTTACTTACGAAGAAGTCT
TCTATTTTGTTAAACGCCAAGACTGGAAGAAACTTATGGAATTCGATCAGTTACAGTTACAGAAATCAAG
TGGAAAAATTTTTAAAGACTTTCATGAAGGAGCCGTTAACTTCGGACCCACCTACAAGTATGACGTTGGA
TCAGCTGCCTACGACACAAGTGACAAGTGCCGTACCCCAGCCTGGACAGACAGGGTGCTGTGGTGGAGGA
AGAAGCATCCATATGATAAAACAGCTGGTGAACTCAACCTTCTAGACAGCGATCTAGACGGCGATCCCCA
AATCAGACACACCTGGTCTCCAGGCACTCTGAAATACTACGGCCGTGCAGAGCTGCAGGCGTCTGATCAC
AGACCTGTGCTGGCCATTGTGGAGGTGGAGGTTCAAGAGGTGGATGTAGGAGCCCGGGAGAGGGTCTTCC
AGGAAGTGTCCTCTGTCCAAGGCCCGCTGGATGCCACCGTTGTTGTAAACCTCCAGTCTCCAACCCTAGA
AGAGAAAAATGAATTTCCAGAGGACCTGCGCACAGAACTTATGCAGACTTTGGGGAATTATGGGACGATT
ATTCTAGTCAGGATCAACCAAGGGCAGATGCTGGTGACGTTTGCAGACAGCCACTCGGCTCTCAGTGTGC
TGGATGTGGATGGTATGAAGGTGAAAGGCAGGGCCGTGAAGATTCGACCAAAGACCAAAGATTGGCTAGA
AGGCCTGAGAGAGGAGCTTCTCCGGAAGCGAGACAGCATGGCCCCTGTGTCTCCCACCGCCAACTCCTGC
TTGTTGGAGGAGAACTTTGACTTCTCGAGTCTGGACTATGAGTCCGAAGGGGATGTTCTTGAAGAGGATG
AAGACTATTTAGTGGATGGGTTTGGCCAGCCTGTAGTCTCAGACAGTGAGCTCGGTGGAGACAACTCTTC
CGACACCATGAGCTCCTTGACACCCGCCAGCAAGTCTCCCGCCCTGGCTAAAAAGAAGCAACATCCAACA
TACAAAGCTGGCTTAATGGTGAAGAAGTCAGCCTCAGACGCGTCCATCTCTTCTGGCACTCATGGACAAT
ATTCCATCTTGCAGACAGCGAAACTTCTCCCAGGAGCACCCCAGCAACCACCCAAGGCTAGAACTGGAAT
AAGTAAACCTTACAACGTCAAACAGATCAAAACCACCAACGCTCAGGAGGCAGAAGCAGCTATCCGGTGT
CTCCTGGAAGCTAGCGGAGGGGTCCCGGAATCAGCCCCAGGTGCCATACCCCTGAGAAACCAAGGGTCTT
CTAAGCCAGAGGCCACCCTGGGGCCCCCAGCCCTGCCCCGCCGGCCTGCTCCAAGGGTTCCCACTATGAA
GAAACCAACTTTGAGGAGGACAGGAAAGCCCATGTTACCAGAAGAAAACTTTGAGCCACAGCCTGTCCAT
TTTACAATGGCTTCCCAGGAAATGAACCTTGAGACCCCTCCTCCAATAACAGCTACTCCCATCCCGCCTG
TTCCCAAACCAAGAACACTTCAGCCTGGGAAAGGTGTTGAGGGGAGGCCAAGCAGCGGTAAGCCAGAACC
AGATGAAGCTCCTTCCGTGACAGGCACTGTGGAGTCACCACCTCCAGAGGCCCAGGAGGCCCCGTCTCTG
GCTCCCAAGGTGCCTCCGAGGAGGAAGAAGTCTGCACCAGCGGCCTTCCACCTGCAGGTCCTGCAGAACA
ACAGCCAAGTTCTCCAGGGCCTCACGTGCTCCAGCAGTTCTCCACCCTCACTGAAGCCCGACACCCACCC
GCTCTGTCTACAAGTGGCACTTGGCACTTCATCTGCCAGAAGCCCTGAAACCCATGGCCCCAGAGTGACA
GAGCCCGAGGCGGCCTCTTTTCATGGCAACTATCCAGATCCCTTCTGGAGCCTTCTCCACCACCCTAAGC
TGTTGAACAATACCTGGCTGTCCAAGAGCTCCGAGCCTTTAGACGTGGGGTCCAGGAACCCTGAGAGGAC
ACACACAGAGCCGGCACAGGTCAATGCATCGTTGGCTGAGAGGGGGCTTCCACCAGACCATGGGGGAAAA
GACTTAAGTCACTGGGTGACAGCTAGTAACAAAGACAAGAGGACAACATTAGGTGTT


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR211941 representing BC060214
Red=Cloning site Green=Tags(s)

MSVGRIPDAEIYKITATELYPLQEEAKEEDRLPTLKKILSSGVFYFAWPNDGACFDLTIRAQKQGDDGSE
WGTSFFWNQLLHVPLRQHQVNCHNWLLKVICGVVTIRTVYASHKQAKACLISRISCERAGARFLTRGVND
DGHVSNFVETEQTIYMDDGVSSFVQIRGSVPLFWEQPGLQVGSHHLRLHRGLEANAPAFERHMVLLKEQY
GKQVVVNLLGSRGGEEVLNRAFKKLLWASCHAGDTPMINFDFHQFAKGRKLEKLENLLRPQLQLHWEDFG
VFAKGENVSPRFQKGTLRMNCLDCLDRTNTVQCFIALEVLHLQLESLGLNSKPIIDRFVESFKAMWSLNG
HSLSKVFTGSRALEGKAKVGKLKDGARSMSRTIQSNFFDGVKQEAIKLLLVGDVYNEESTDKGRMLLDNT
ALLATPRILKAMTERQSEFTNFKRIQIAVGTWNVNGGKQFRSNLLGTAELTDWLLDAPQLSGAVDSQDDG
SPADVFAIGFEEMVELSAGNIVNASTTNRKMWGEQLQKAISRSHRYILLTSAQLVGVCLYIFVRPYHVPF
IRDVAIDTVKTGMGGKAGNKGAVGIRFQLHSTSFCFVCSHLTAGQSQVKERNEDYREITHKLSFPSGRNI
FSHDYVFWCGDFNYRIDLTYEEVFYFVKRQDWKKLMEFDQLQLQKSSGKIFKDFHEGAVNFGPTYKYDVG
SAAYDTSDKCRTPAWTDRVLWWRKKHPYDKTAGELNLLDSDLDGDPQIRHTWSPGTLKYYGRAELQASDH
RPVLAIVEVEVQEVDVGARERVFQEVSSVQGPLDATVVVNLQSPTLEEKNEFPEDLRTELMQTLGNYGTI
ILVRINQGQMLVTFADSHSALSVLDVDGMKVKGRAVKIRPKTKDWLEGLREELLRKRDSMAPVSPTANSC
LLEENFDFSSLDYESEGDVLEEDEDYLVDGFGQPVVSDSELGGDNSSDTMSSLTPASKSPALAKKKQHPT
YKAGLMVKKSASDASISSGTHGQYSILQTAKLLPGAPQQPPKARTGISKPYNVKQIKTTNAQEAEAAIRC
LLEASGGVPESAPGAIPLRNQGSSKPEATLGPPALPRRPAPRVPTMKKPTLRRTGKPMLPEENFEPQPVH
FTMASQEMNLETPPPITATPIPPVPKPRTLQPGKGVEGRPSSGKPEPDEAPSVTGTVESPPPEAQEAPSL
APKVPPRRKKSAPAAFHLQVLQNNSQVLQGLTCSSSSPPSLKPDTHPLCLQVALGTSSARSPETHGPRVT
EPEAASFHGNYPDPFWSLLHHPKLLNNTWLSKSSEPLDVGSRNPERTHTEPAQVNASLAERGLPPDHGGK
DLSHWVTASNKDKRTTLGV

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060214
ORF Size 4047 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060214.1
RefSeq Size 4694 bp
RefSeq ORF 4049 bp
Locus ID 20975
MW 149.6 kDa
Gene Summary Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...