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Dhx30 (BC016202) Mouse Tagged Lenti ORF Clone
CAT＃: MR211825L4
- LentiORF®
Lenti ORF clone of Dhx30 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765)
"BC016202" in other vectors (4)
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CNY 8,840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | HELG, Ddx30 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR211825).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC016202 |
| ORF Size | 3669 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016202.1 |
| RefSeq Size | 3768 bp |
| RefSeq ORF | 3671 bp |
| Locus ID | 72831 |
| Gene Summary | RNA-dependent helicase (PubMed:25219788). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (By similarity). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (PubMed:25219788).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223099 | Dhx30 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765), (10ug) |
CNY 12,920.00 |
|
| MG211825 | Dhx30 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765) |
CNY 7,600.00 |
|
| MR211825 | Dhx30 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765) |
CNY 8,520.00 |
|
| MR211825L3 | Lenti ORF clone of Dhx30 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765) |
CNY 8,840.00 |
