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Dhx30 (BC016202) Mouse Tagged ORF Clone
CAT＃: MR211825
- TrueORF®
Dhx30 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765)
ORF Plasmid: tGFP
"BC016202" in other vectors (4)
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CNY 8,520.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HELG, Ddx30 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211825 representing BC016202
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCCAGGCGGCTCATGGCGCTGGCTGCCGGCGTCTCTCCGCGCCTCCGGCCGCCGGATCCCC TTGTCGCTTCCGGGCGACAAGGATGTTCGCGCGGCTTCTCATCAAGCTTTGTCCGCTCTGATGGCACTCA GGAAGCTGCCGAGGTCGAATCAGAGGTGGCCCCTAGCGAGCCTGGGGAGGGTGACGGAAGCATGGTGAAC GCTTCTAGGGACCTATTAAAAGAGTTTCCGCAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCCC TTGGCATCTCACATGCAAAAGACAAGTTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCAC CCTGCACATAAAGTGGCCCAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATTGATGCTGAGCGT CAGGCTGCAGCAGCTGCCTGCCAACTCTTCAAGGGCTGGGGTCTGCTGGGACCACGGAATGAGCTGTTTG ATGCAGCTAAATACCGAGTGCTAGCTGATCGTTTTGGGTCTCCAGCTGACAGCTGGTGGCGCCCAGAACC CACCATGCCTCCAACTTCCTGGCGGCAGCTGAATCCTGAGAACATTCGGCCAGGGGGTCCTGCAGGACTA TCCCGATCCTTAGGCCGAGAGGAAGAGGAAGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGA CAGAGTTTCTGTCTATGACCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGA AATGACAGATGATGACAGTGCTATCAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTCCTGGCCAAA GTGATTCAGATTGCAACCTCCTCCTCCACAGCTAAGAATCTCATGCAGTTCCATACTGTGGGTACCAAGA CCAAGCTGGCTACACTCACTCTGCTCTGGCCCTGTCCCATGACCTTTGTTGCCAAAGGGCGACGCAAAGC TGAGGCTGAGAATAAGGCAGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTTGGCCTTGTGGACAGGAAC AATGAGCCGCTTACCCATGCCATGTACAACCTGGCCTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGC CATGTACCATCCAGGTGCCTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGA CAGCTCATGGATTTCCCCAGAACTCCGACTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGG CCCTTGAGTGACCCTATCACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCTTAAGCC AGAGCCTGCTAGAGCTGTGGCGGAGGAGAGGGCCGATCTGGCAGGAGGCCCCGCAGCTACCTGTAGACCC TCATCGGGACACTATCCTCAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGT GGGAAGACCACACGTATCCCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCA ATGTGATCATCACACAACCTCGCCGGATCTCAGCTGTGTCTGTGGCACAGCGGGTCAGCCATGAACTGGG CCCCTCCTTGCGCCGGAATGTGGGCTTCCAGGTACGCTTGGAAAGTAAGCCCCCAGCCCGAGGTGGGGCG CTGCTCTTCTGCACTGTGGGTATCCTGCTTCGGAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCC ATGTCATTGTGGATGAGGTCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCT GCAGCGGCTCAACCCAGCTCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGA TACTTTGGGGGCTGCCCTGTTATCAAGGTACCTGGCTTCATGTATCCCGTCAAGGAACACTACCTGGAGG ACATCTTGGCCAAGCTGGGTAAACATCAGTACCCACACCGGCATCGGCACCATGAGTCGGAAGATGAATG TGCACTTGATTTGGACCTTGTGACTGACCTGGTTCTGCATATCGATGCCCGTGGGGAACCAGGTGGGATC CTGTGCTTTCTACCTGGCTGGCAAGAAATCAAAGGAGTGCAACAACGGCTCCAGGAGGCCCTGGGCATGC ATGAAAGCAAGTACCTCATCTTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCA ACAGCCTCCACTTGGGGTACGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAAC GACATTGTACATGTCGTGGACAGCGGTCTGCACAAGGAGGAACGCTATGACCTGAAGACCAAGGTGTCCT GCCTGGAGACTGTGTGGGTGTCGAGAGCAAATGTCATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTC AGGTTTTGCCTACCACTTGTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATC CTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCCAAAATCCATATGCCTGAGAAGACGGCAGTGGAGT TCCTCTCTAAGGCTGTGGACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATCCTGCTCCAGGAGAT TGGGGTGCTGGACCAGCGGGAGTATCTGACCACCTTGGGACAGCGCCTGGCCCACATCTCTACTGACCCC CGACTGGCCAAGGCCATAGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCT GCCTTACCCGGGACCCCTTCAGCAGCAGTTTGCAGAACCGGGCAGAAGTAGACAAGGTGAAGGCATTGCT GAGCCATGACAGTGGCAGTGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGC TGGCAGGACCGTACCTCCAGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCA TCCACGGGCTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCTTTCCTAGTGGGGAAGCCCTCTGACTG CACACTGCCCTCTGCTCAGTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCT GGCCTCTACCCCAACCTCATCCAGGTGAGACAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACA GTGTCACTTACAGGACCAAATCTGGCAACATCTTGCTGCATAAGTCAACCATTAACAGGGAGGCTACCCG GTTACGGAGCCGATGGCTGACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCC TCCCAGGTGCACCCACTAGCTGTGTTGCTCCTAACAGATGGGGACGTGCACATCCGAGATGATGGGCGTC GGGCCACCATCTCATTGAGTGACAGCGACCTGCTTCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCT AAGGGAGTTTCGTCGAGCCCTAGGACGGATGGTGGAGCGGAGCCTCCGCAGCGAGTTAGCTGCACTTCCT CTTAGTGTGCAGCAAGAACACGGGCAGCTGCTTGCACTGCTGGCAGAGTTGCTTCGAGGACCTTGTGGCA GCTTTGATATGCGCAAGACAGCTGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211825 representing BC016202
Red=Cloning site Green=Tags(s) MAAARRLMALAAGVSPRLRPPDPLVASGRQGCSRGFSSSFVRSDGTQEAAEVESEVAPSEPGEGDGSMVN ASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAER QAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGL SRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAK VIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRN NEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSG PLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGC GKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGA LLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSR YFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGI LCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVN DIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEI LRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDP RLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLR WQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMA GLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDS SQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALP LSVQQEHGQLLALLAELLRGPCGSFDMRKTADD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC016202 |
| ORF Size | 3669 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016202.1 |
| RefSeq Size | 3768 bp |
| RefSeq ORF | 3671 bp |
| Locus ID | 72831 |
| MW | 138.2 kDa |
| Gene Summary | RNA-dependent helicase (PubMed:25219788). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (By similarity). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (PubMed:25219788).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223099 | Dhx30 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765), (10ug) |
CNY 12,920.00 |
|
| MG211825 | Dhx30 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765) |
CNY 7,600.00 |
|
| MR211825L3 | Lenti ORF clone of Dhx30 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765) |
CNY 8,840.00 |
|
| MR211825L4 | Lenti ORF clone of Dhx30 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765) |
CNY 8,840.00 |
