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Wdr11 (NM_172255) Mouse Tagged ORF Clone
CAT＃: MR211823
- TrueORF®
Wdr11 (Myc-DDK-tagged) - Mouse WD repeat domain 11 (Wdr11)
ORF Plasmid: tGFP
"NM_172255" in other vectors (4)
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CNY 8,520.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2900055P10Rik; AW489876; Brwd2; mKIAA1351 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211823 representing NM_172255
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTACCCTACACCGTAAACTTCAAGGTGTCAGCGCGCACCCTCACCGGGGCTCTCAACGCGCACAACA AGGCGGCGGTGGACTGGGGCTGGCAAGGTTTAATTGCATATGGATGCCATTCACTGGTGGTAGTGATCGA TTCCAATACTGCCCAAACTCTACAAGTTCTAGAAAAACATAAAGCTGATATTGTTAAGGTCAGGTGGGCC AGGGAGAACTATCACCATAACATCGGCTCTCCATACTGCCTGCGCTTGGCTTCTGCGGATGTCACTGGGA AGATCATTGTCTGGGATGTAGCAGCCGGAGTAGCCCAGTGTGAGATCCAAGAGCACGTGAAGCCCATCCA AGATGTGCAGTGGCTGTGGAATCAGGACGCTTCCCGAGACTTGCTGCTCGCTATCCACCCGCCTAATTAC ATCGTGCTGTGGAATGCAGACACAGGCACCAAGCTGTGGAAGAAGAGCTACGCAGATAACATCCTTTCTT TTTCTTTTGACCCTTTTGATCCTTCACATTTAACTTTACTCACCAGCGAGGGCATCGTTTTCATCTCAGA CTTCTCTCCATCCAAGCCTCCCTCCGGCCCTGGGAAGAAAGTGTACATCTCCAGCCCACACTCCAGCCCA GCTCATAACAAGCTGGCCGCAGCCACGGGAGCCAAGAAGGCTCTTAATAAAGTAAAGATTTTAATCACTC AGGAGAAACCGAGTGCTGATTTTGTAGCTCTCAATGACTGCCTCCAGTTAGCCTATCTGCCTTCAAAAAG GAATCACATGCTGTTGCTCTACCCTCGGGAGATTTTAATCCTTGACCTGGAAGTGAATCAAACAGTTGGT GTGATTGCAATCGAGAGAACAGGCGTTCCCTTTCTGCAGGTAATACCATGCTCTCAGCGAGATGGCTTGT TTTGCCTACATGAAAATGGCTGCATAACCTTACGTGTCCGGAGGTCTTATAACAGCATCTGTACTACATC AAATGATGAACCAGATCTAGATCCTGTCCAGGAGCTTACCTATGACTTGAGAAGCCAGTGTGATGCAATC AGGGTGACGAAAACTGTCCGTCCCTTCAGTATGGTGTGCTGTCCTGTTAATGAGAATGCAGCTGCCCTCA TAGTAAGCGACGGCAGAGTCATGATATGGGAACTCAAGTCTGCTGTGTGCAGTCGAAATGCACGGAACAG TTCCGGTGTGTCTCCTTTATACTCGCCAGTGTCCTTCTGTGGGATTCCTGGAGGAGTGCTACAGAATAAA CTCCCAGACCTTTCCCTAGATAATATGATCGGGCAGAGTGCAATTGCTGGGGAAGAACACCCCAAAGGCT CCATTCTGCAGGAAGTGCACCTCAAGTTCCTGCTGACGGGACTGCTCTCAGGACTCCCTTCCCCTCAGTT TGCCATCCGAATGTGCCCACCACTGACCACAAAAAACATTAAGATGTACCAGCCACTCCTTGCTGTTGGT ACGAGTAACGGTTCTGTCCTGGTGTACCACCTCACCAGTGGCCTGCTGCACAAGGAGCTCAGTGTCCACT CGTGTGAAGTCAAGGGTATTGAATGGACAAGCTTGACCAGTTTCCTTTCTTTTGCTGCTTCGACCCCAAA CAACATGGGGTTAGTAAGAAATGAACTTCAGCTGGTTGATCTCCCAACAGGGAGGAGCACTGCTTTCCGT GGTGACCGGGGCAATGACGAATCACCCATTGAAATGATCAAAGTGTCTCATCTGAAACAGTATTTGGCAG TTGTTTTCAAAGATAAGCCCCTGGAATTATGGGATATTAGAACTTGCACTCTTCTTAGAGAAATGTCTAA AAGTTTCCCTGCTATAACTGCATTGGAGTGGTCACCATCTCACAACCTGAAGAGCCTGAGAAAGAAGCAG CTTGCCACCCGGGAGGCCATGGCCCGCCAGACTGTGGTCTCCGATGCAGAGCTGGGTGCTGTTGAATCAT CTGTGATCAGTTTATTACAGGAGGCAGAGAGTAAGGCTGAACTCAGTCAGAACATCTCTGCTCGGGAGCA TTTTGTGTTTACTGACAATGATGGCCAAGTTTATCATCTCACTGTTGAAGGAAATTCTGTGAAAGACAGT GCTCGGATTCCACCAGATGGTAGCATGGGCAGTATTACCTGCATCGCTTGGAAGGGGGATACATTAGTGC TTGGAGATATGGATGGAAATTTAAACTTCTGGGATTTGAAAGCCAGAGTATCCAGAGGAATACCCACACA TAGAAGTTGGGTGAGGAAGATTCGCTTTGCCCCCGGCAAGGGAAACCAGAAGCTAATAGCAATGTATAAT GATGGTGCTGAAGTGTGGGATACTAAAGAGGTTCAGATGGTGAGCAGTTTAAGAAGTGGAAGAAATGTAA CCTTCCGGATTTTGGATGTGGACTGGTGCACATCAGATAAGGTGATCTTGGCATCTGATGACGGCTGCAT CAGAGTCCTGGAGATGTCAATGAAGTCTACGTGTTTCAGAATGGATGAGCAGGAGTTAGTAGAGCCTGTG TGGTGCCCGTACCTCCTTGTTCCGAGGGCTGCCCTTGCTTTGAAAGCCTTCCTACTGCATCAGCCTTGGA ATGGGCGATATTCGTTGGACATTTCTCACATTGATTATCCAGAAAATGAAGAAATAAAGACTCTCCTTCA AGAACAGTTGCATGCATTGTCTAACGACATAAAGAAACTCCTGCTTGATCCCGACTTCTCTCTCTTGCAG AGGTGCCTGCTCGTGTCCAGGCTGTACGGCGATGAATCGGAGCTGCACTTCTGGACAGTGGCGGCCCACT ACCTGCACAGCTTGTCCCAGGCCAAGTCTGGGGACACAGTGGTAACCAAGGAAGGTGCTCCTAAGGACAG GCTGAGCAACCCATTGGACATCTGCTACGATGTGCTCTGTGAGAACACCTACTTCCAGAAATTTCAGCTA GAAAGAGTTAATCTACAGGAAGTAAAACGGTCAACTTATGATCACACGAGAAAGTGCACAGACCAGCTCC TGCTGCTAGGCCAGACAGACAGAGCTGTGCAGCTGCTGTTGGAAACAAGTGCAGATAACCAGCACTACTA CTGCGACTCTCTGAAAGCTTGCTTGGTCACCACTGTCACCTCTTCAGGCCCTTCTCAGAGCACAATTAAG CTGGTAGCAACCAATATGATCGCCAATGGCAAGCTGGCAGAGGGTGTTCAGTTACTCTGCTTGATAGACA AGGCTGCGGACGCCTGTCGCTACCTGCAGACGTATGGCGAGTGGAATCGGGCGGCATGGCTTGCAAAGGT TCGGTTAAATTCTGAAGAATGTGCAGATGTTCTGAAGCGGTGGGTTGACCACCTTTGTTCTCCACAAGTC AACCAGAAGTCCAAGGCCCTCCTGGTCCTCCTCTCTCTGGGTTGCTTTGTCAGCGTGGCAGAGACGCTTC ACAGCATGAGATACTTCGATAGAGCTGCCTTGTTTGTGGAAGCCTGTCTCAAGTACGGTGCATTTGAAGT CAGTGAGGACACAGAGAAGCTCATTGCTGCCATCTACGCAGACTATGCCCGGAGCCTGAAGAGCCTCGGC TTTAGACAGGGAGCAGTGCGTTTTGCTTCAAAAGCTGGAGCGGCTGGCAGAGATTTGTTGAATGAGCTTG GGTCCACCAAGGAAGAGCTAACGGAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211823 representing NM_172255
Red=Cloning site Green=Tags(s) MLPYTVNFKVSARTLTGALNAHNKAAVDWGWQGLIAYGCHSLVVVIDSNTAQTLQVLEKHKADIVKVRWA RENYHHNIGSPYCLRLASADVTGKIIVWDVAAGVAQCEIQEHVKPIQDVQWLWNQDASRDLLLAIHPPNY IVLWNADTGTKLWKKSYADNILSFSFDPFDPSHLTLLTSEGIVFISDFSPSKPPSGPGKKVYISSPHSSP AHNKLAAATGAKKALNKVKILITQEKPSADFVALNDCLQLAYLPSKRNHMLLLYPREILILDLEVNQTVG VIAIERTGVPFLQVIPCSQRDGLFCLHENGCITLRVRRSYNSICTTSNDEPDLDPVQELTYDLRSQCDAI RVTKTVRPFSMVCCPVNENAAALIVSDGRVMIWELKSAVCSRNARNSSGVSPLYSPVSFCGIPGGVLQNK LPDLSLDNMIGQSAIAGEEHPKGSILQEVHLKFLLTGLLSGLPSPQFAIRMCPPLTTKNIKMYQPLLAVG TSNGSVLVYHLTSGLLHKELSVHSCEVKGIEWTSLTSFLSFAASTPNNMGLVRNELQLVDLPTGRSTAFR GDRGNDESPIEMIKVSHLKQYLAVVFKDKPLELWDIRTCTLLREMSKSFPAITALEWSPSHNLKSLRKKQ LATREAMARQTVVSDAELGAVESSVISLLQEAESKAELSQNISAREHFVFTDNDGQVYHLTVEGNSVKDS ARIPPDGSMGSITCIAWKGDTLVLGDMDGNLNFWDLKARVSRGIPTHRSWVRKIRFAPGKGNQKLIAMYN DGAEVWDTKEVQMVSSLRSGRNVTFRILDVDWCTSDKVILASDDGCIRVLEMSMKSTCFRMDEQELVEPV WCPYLLVPRAALALKAFLLHQPWNGRYSLDISHIDYPENEEIKTLLQEQLHALSNDIKKLLLDPDFSLLQ RCLLVSRLYGDESELHFWTVAAHYLHSLSQAKSGDTVVTKEGAPKDRLSNPLDICYDVLCENTYFQKFQL ERVNLQEVKRSTYDHTRKCTDQLLLLGQTDRAVQLLLETSADNQHYYCDSLKACLVTTVTSSGPSQSTIK LVATNMIANGKLAEGVQLLCLIDKAADACRYLQTYGEWNRAAWLAKVRLNSEECADVLKRWVDHLCSPQV NQKSKALLVLLSLGCFVSVAETLHSMRYFDRAALFVEACLKYGAFEVSEDTEKLIAAIYADYARSLKSLG FRQGAVRFASKAGAAGRDLLNELGSTKEELTES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172255 |
| ORF Size | 3669 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172255.3, NP_758459.2 |
| RefSeq Size | 4566 bp |
| RefSeq ORF | 3672 bp |
| Locus ID | 207425 |
| UniProt ID | Q8K1X1 |
| MW | 136 kDa |
| Gene Summary | Involved in the Hedgehog (Hh) signaling pathway, is essential for normal ciliogenesis (PubMed:29263200). Regulates the proteolytic processing of GLI3 and cooperates with the transcription factor EMX1 in the induction of downstream Hh pathway gene expression and gonadotropin-releasing hormone production (PubMed:29263200). WDR11 complex facilitates the tethering of Adaptor protein-1 complex (AP-1)-derived vesicles. WDR11 complex acts together with TBC1D23 to facilitate the golgin-mediated capture of vesicles generated using AP-1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223992 | Wdr11 (untagged) - Mouse WD repeat domain 11 (Wdr11), (10ug) |
CNY 15,680.00 |
|
| MG211823 | Wdr11 (tGFP-tagged) - Mouse bromodomain and WD repeat domain containing 2 (Brwd2) |
CNY 10,550.00 |
|
| MR211823L3 | Lenti ORF clone of Wdr11 (Myc-DDK-tagged) - Mouse WD repeat domain 11 (Wdr11) |
CNY 11,500.00 |
|
| MR211823L4 | Lenti ORF clone of Wdr11 (mGFP-tagged) - Mouse WD repeat domain 11 (Wdr11) |
CNY 11,500.00 |
