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Nos3 (BC052636) Mouse Tagged ORF Clone
CAT＃: MR211798
- TrueORF®
Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765)
ORF Plasmid: tGFP
"BC052636" in other vectors (4)
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CNY 8,368.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | eNOS, ecNOS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211798 representing BC052636
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGTTTAG GGCTGTGCGGCAAGCAGGGCCCAGCCTCTCCAGCACCGGAGCCTAGCCAGGCGCCAGCACCCCCGTCCCC AACCCGACCAGCACCAGACCACAGCCCCCCGCTAACCCGGCCCCCAGACGGACCCAGGTTTCCTCGAGTA AAGAATTGGGAAGTGGGCAGCATCACCTACGACACCCTCAGTGCCCAGGCTCAGCAGGATGGGCCCTGTA CCTCAAGACGCTGCTTGGGATCCCTGGTGTTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCCATCAAAAGGAGT GGCTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAGGCTGAGGTGGCAGCCACAGGCACCTACCAGC TCCGGGAGAGCGAGCTGGTGTTTGGGGCCAAGCAGGCCTGGCGCAATGCTCCCCGCTGTGTGGGCCGGAT CCAGTGGGGAAAGCTGCAGGTATTTGATGCTCGGGACTGCAGGACTGCACAGGAAATGTTCACCTACATC TGTAACCACATTAAATACGCAACAAATAGAGGCAATCTTCGTTCAGCCATCACAGTGTTCCCCCAGCGCT GCCCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGATACGCTATGCGGGCTATAGGCAGCAGGA TGGCTCCGTGCGAGGGGACCCCGCCAACGTGGAGATCACTGAGCTCTGTATCCAACATGGCTGGACCCCA GGAAATGGCCGCTTTGATGTGCTGCCCCTGTTACTCCAGGCTCCTGATGAGCCCCCAGAACTCTTCACTC TGCCCCCAGAGATGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTCGAGTGGTTTGCTGCCCTTGGCCT GCGCTGGTATGCCCTCCCAGCTGTGTCCAACATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCTGCTGCC CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGCTACAACA TACTTGAGGATGTGGCTGTGTGCATGGATCTGGACACCAGGACAACCTCATCCCTGTGGAAAGACAAGGC AGCGGTGGAAATTAATGTGGCCGTGTTGCACAGTTACCAGCTGGCCAAAGTGACCATAGTGGACCACCAC GCCGCCACAGCCTCCTTCATGAAGCACCTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCTGCCGATT GGGCCTGGATTGTGCCCCCCATCTCAGGCAGCCTAACTCCTGTCTTCCATCAAGAGATGGTCAACTATTT CCTGTCCCCTGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGAAGTGCAGCAAAGGGGGCAGGCATCACC AGGAAGAAGACCTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG CGAAGCGTGTGAAGGCAACCATTCTGTATGGCTCTGAGACTGGCCGGGCCCAGAGCTACGCACAGCAGCT GGGAAGACTCTTCCGGAAGGCGTTTGATCCCCGGGTCCTGTGCATGGATGAGTATGATGTGGTGTCCCTA GAGCACGAGGCACTGGTGTTGGTGGTGACCAGCACATTTGGCAATGGGGATCCTCCGGAGAATGGAGAGA GCTTTGCAGCAGCGCTCATGGAAATGTCAGGCCCGTACAACAGCTCCCCTAGGCCTGAGCAGCACAAGAG CTACAAAATCCGATTCAACAGTGTCTCCTGCTCAGACCCACTGGTATCCTCTTGGCGGCGCAAGAGGAAG GAGTCTAGCAACACAGACAGTGCAGGAGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCC GAGCATACCCCCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG ACTACTGCAGCTGGGCCAAGGTGATGAGCTCTGTGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCC GCCTTCCAGGCTGCCTGTGAAACCTTCTGTGTGGGAGAAGATGCCAAAGCTGCTGCCCGAGATATCTTCA GCCCCAAACGCAGCTGGAAGCGCCAGAGGTACCGGCTGAGTACCCAGGCTGAGAGCCTGCAATTACTACC AGGGCTGACTCACGTGCACAGGCGGAAGATGTTCCAGGCTACAATCCTCTCTGTGGAAAACCTACAGAGC AGCAAATCCACCCGAGCCACGATCCTGGTGCGTCTGGACACCGGAGGCCAGGAGGGACTGCAGTACCAGC CAGGGGACCACATAGGTGTGTGCCCACCCAACCGTCCTGGCCTAGTGGAGGCACTGCTGAGCCGAGTGGA GGACCCTCCGCCATCCACAGAACCTGTGGCTGTGGAACAACTGGAGAAAGGCAGCCCTGGTGGCCCTCCC CCCGGCTGGGTACGGGACCCCCGGCTACCCCCATGTACGCTGCGGCAGGCTCTCACCTACTTCCTGGACA TCACTTCCCCGCCTAGTCCTCGCCTCCTTCGACTGCTCAGCACCCTGGCAGAAGAGTCCAGCGAACAGCA GGAGCTAGAGGCTCTCAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCAGCTGCCCCACACTG CTAGAGGTGCTGGAGCAATTTCCTTCAGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCTTGC TCCAGCCCCGGTACTACTCTGTCAGTTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACCATAGC TGTGCTGGCTTACAGAACCCAGGATGGGCTGGGCCCTCTGCACTACGGTGTCTGCTCCACGTGGATGAGC CAGCTCAAGGCGGGAGATCCAGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC CTAACTTGCCCTGCATCCTGGTGGGCCCAGGGACTGGCATTGCACCCTTCCGGGGATTCTGGCAAGACAG ACTACACGACATTGAGATCAAAGGGCTACAACCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGGGTGTTTGGACAAGTCCTCA CCGCCTTTTCCAGGGATCCTGGCAGCCCCAAGACCTACGTGCAAGACCTCCTGAGGACAGAGCTAGCCGC GGAGGTTCACCGTGTGCTGTGCCTTGAGCAAGGACATATGTTTGTCTGCGGCGATGTCACTATGGCAACC AGCGTCCTGCAAACCGTGCAGAGAATTCTGGCAACAGAGGGCGGCATGGAGCTGGATGAAGCCGGTGACG TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA GGTGACAAGCCGCATACGCACCCAGAGCTTTTCTTTGCAGGAGCGACAGCTGAGGGGCGCAGTGCCCTGG TCCTTTGACCCGCCTGGCCCAGAAATACCTGGTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211798 representing BC052636
Red=Cloning site Green=Tags(s) MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSQAPAPPSPTRPAPDHSPPLTRPPDGPRFPRV KNWEVGSITYDTLSAQAQQDGPCTSRRCLGSLVFPRKLQSRPTQGPSPTEQLLGQARDFINQYYNSIKRS GSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRTAQEMFTYI CNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLIRYAGYRQQDGSVRGDPANVEITELCIQHGWTP GNGRFDVLPLLLQAPDEPPELFTLPPEMVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAA PFSGWYMSSEIGMRDLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHH AATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGAGIT RKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSL EHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRK ESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQA AFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQAESLQLLPGLTHVHRRKMFQATILSVENLQS SKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPSTEPVAVEQLEKGSPGGPP PGWVRDPRLPPCTLRQALTYFLDITSPPSPRLLRLLSTLAEESSEQQELEALSQDPRRYEEWKWFSCPTL LEVLEQFPSVALPAPLILTQLPLLQPRYYSVSSAPSAHPGEIHLTIAVLAYRTQDGLGPLHYGVCSTWMS QLKAGDPVPCFIRGAPSFRLPPDPNLPCILVGPGTGIAPFRGFWQDRLHDIEIKGLQPAPMTLVFGCRCS QLDHLYRDEVLDAQQRGVFGQVLTAFSRDPGSPKTYVQDLLRTELAAEVHRVLCLEQGHMFVCGDVTMAT SVLQTVQRILATEGGMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPW SFDPPGPEIPGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052636 |
| ORF Size | 3606 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052636.1 |
| RefSeq Size | 3982 bp |
| RefSeq ORF | 3608 bp |
| Locus ID | 18127 |
| MW | 146 kDa |
| Gene Summary | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223060 | Nos3 (untagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765), (10ug) |
CNY 12,920.00 |
|
| MG211798 | Nos3 (tGFP-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) |
CNY 7,510.00 |
|
| MR211798L3 | Lenti ORF clone of Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) |
CNY 8,740.00 |
|
| MR211798L4 | Lenti ORF clone of Nos3 (mGFP-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) |
CNY 8,740.00 |
