Egf (BC017681) Mouse Tagged ORF Clone

CAT#: MR211795

  • TrueORF®

Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592)

ORF Plasmid: DDK tGFP



  "BC017681" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 11,970.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI790464
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211795 representing BC017681
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATAC
TCAGCGAGAGAAGCGGGACTTGTGCCGGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTC
TCGGATTGACCCAGATGGAACAAATCACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGAT
ATTCATTATAAAAAAGAGAGACTCTATTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTA
ACGGGACAGGACTAGAGAAAGTGTGCAATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGA
TGATGAAGTTCTCTGGGTAGACCAACAGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCC
CGAGTTCTTCTAAGTTCCTTAAAACATCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTT
GGTCTTCAGAGGTGACCGGCAGCCTTCACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGA
GACAGGGGGAATATCGGTGCTGACTCTGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGC
GAAGGAAGCCACGCTTACATTCATTCCTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAG
CACGGCACAGTTTGTCTTCAATGGCCTTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGC
GATTTGGATAGCCAACAAACACACGGGGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACA
CCTGGAAAACTGATGGTAGTACACCCTCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACC
CCGAACTTCTCAAACAGAGGGGAAGACCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTC
GAGCGCATGCGCTGAGGGCTACACGTTAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCC
ACTCAGAATCACGGCTGTACTCTTGGGTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAG
GATTTGTTCTGCTTCCTGATGGGAAACAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTG
CAGTCATGGCTGTGTCCTGACATCAGATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGA
GATGGGAAGACTTGCACTGGTTGTTCATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCA
GGCCAGGATCCTGGGAATGTGATTGCTTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGC
TTCAGGACCACAGCCACTTTTACTGTTTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACA
GACTACAAAGTTCTGCTCAGCCGGCAGATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCA
AGATATATTTTGCACAGACAGCCCTGAAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAG
ACTGATCACAGAAGGAGTAGATACGCTTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGG
ACAGACAGTGGGAAGTCTGTTGTTGGAGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGG
AGAGAATCTCGAGGCCGCGAGGAATAGCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGG
GATGTCTCCACGGATTGAAAGCGCTTCCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTA
CTGGAACCCAGTGGAATCACGATTGACTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTG
TGATTGAAATGGCCAATCTGGATGGCTCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTT
CTCTCTAGCCGTGTTTGAGGATCACCTGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAAC
AAGAGGACTGGCCAAAACAGGGTACGTCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCC
ATCCATTGGCAAAACCAGGTGCAGATCCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGA
GAGCCTGGGCACAGCTCGGTGTTTGTGTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTC
CCTCAGGATTATCCAATCCTGTCAGGTGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACT
CCACTCAGGCTGAAGTACCAGACGATGATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTC
AGGCATGAACTATGAAGATGACTGTGGTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGA
GAGACTGCTGAGTGTCAGTGTCTGAAAGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGT
GTGTGCTGGCTAGATCGGACTGCCCCAGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTG
CAGATGCTCAGAAGGCTACGAAGGAGACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCG
CACAACTGCGCTGAGAATGCCGCCTGCACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCC
CATCCTCGCCCGGACGGAGTTGCCCTGACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTT
GGACCGAAATAGTTATCCAGGATGCCCATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATG
CATATTGAATCACTGGACAGCTACACATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTC
GAGACCTACGATGGTGGGAGCTGCGTCATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGT
CTGCATGGTGGCACTGGTCCTGCTGCTCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAG
CAGCTATCAAACCCCCCAAAGAACCCTTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACA
GCAGCAGCGGGGCAGCTGTGGCTTCTTGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCC
CAAGAATGGGAGTCTGCCTGCGGATGGTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTG
CAGCTCGGGTCAGTGCATCTGACTTCATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAA
GCTGCTGGATTCCACCATCAAGTGACAGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTA
CAGACCTGTGGGGCCGGAGAAGCTGCATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCA
GACCTGCCACGGCAGACAGAGCCAGTTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211795 representing BC017681
Red=Cloning site Green=Tags(s)

MPWGRRPTWLLLAFLLVFLKISILSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMD
IHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNS
RVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSG
EGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVT
PGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECA
TQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGR
DGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGT
DYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYW
TDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNL
LEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVN
KRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCL
PQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDG
ETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGA
HNCAENAACTNTEGGYNCTCAGRPSSPGRSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCM
HIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLLLGMWGTYYYRTRK
QLSNPPKNPCDEPSGSVSSSGPDSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSL
QLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAP
DLPRQTEPVQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC017681
ORF Size 3600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC017681.1
RefSeq Size 4709 bp
RefSeq ORF 3602 bp
Locus ID 13645
MW 172.6 kDa
Gene Summary This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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