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Egf (BC017681) Mouse Tagged ORF Clone
CAT＃: MG211795
- TrueORF®
Egf (tGFP-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592)
ORF Plasmid: DDK
"BC017681" in other vectors (4)
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CNY 13,210.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI790464 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211795 representing BC017681
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATAC TCAGCGAGAGAAGCGGGACTTGTGCCGGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTC TCGGATTGACCCAGATGGAACAAATCACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGAT ATTCATTATAAAAAAGAGAGACTCTATTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTA ACGGGACAGGACTAGAGAAAGTGTGCAATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGA TGATGAAGTTCTCTGGGTAGACCAACAGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCC CGAGTTCTTCTAAGTTCCTTAAAACATCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTT GGTCTTCAGAGGTGACCGGCAGCCTTCACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGA GACAGGGGGAATATCGGTGCTGACTCTGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGC GAAGGAAGCCACGCTTACATTCATTCCTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAG CACGGCACAGTTTGTCTTCAATGGCCTTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGC GATTTGGATAGCCAACAAACACACGGGGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACA CCTGGAAAACTGATGGTAGTACACCCTCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACC CCGAACTTCTCAAACAGAGGGGAAGACCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTC GAGCGCATGCGCTGAGGGCTACACGTTAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCC ACTCAGAATCACGGCTGTACTCTTGGGTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAG GATTTGTTCTGCTTCCTGATGGGAAACAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTG CAGTCATGGCTGTGTCCTGACATCAGATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGA GATGGGAAGACTTGCACTGGTTGTTCATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCA GGCCAGGATCCTGGGAATGTGATTGCTTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGC TTCAGGACCACAGCCACTTTTACTGTTTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACA GACTACAAAGTTCTGCTCAGCCGGCAGATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCA AGATATATTTTGCACAGACAGCCCTGAAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAG ACTGATCACAGAAGGAGTAGATACGCTTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGG ACAGACAGTGGGAAGTCTGTTGTTGGAGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGG AGAGAATCTCGAGGCCGCGAGGAATAGCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGG GATGTCTCCACGGATTGAAAGCGCTTCCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTA CTGGAACCCAGTGGAATCACGATTGACTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTG TGATTGAAATGGCCAATCTGGATGGCTCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTT CTCTCTAGCCGTGTTTGAGGATCACCTGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAAC AAGAGGACTGGCCAAAACAGGGTACGTCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCC ATCCATTGGCAAAACCAGGTGCAGATCCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGA GAGCCTGGGCACAGCTCGGTGTTTGTGTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTC CCTCAGGATTATCCAATCCTGTCAGGTGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACT CCACTCAGGCTGAAGTACCAGACGATGATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTC AGGCATGAACTATGAAGATGACTGTGGTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGA GAGACTGCTGAGTGTCAGTGTCTGAAAGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGT GTGTGCTGGCTAGATCGGACTGCCCCAGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTG CAGATGCTCAGAAGGCTACGAAGGAGACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCG CACAACTGCGCTGAGAATGCCGCCTGCACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCC CATCCTCGCCCGGACGGAGTTGCCCTGACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTT GGACCGAAATAGTTATCCAGGATGCCCATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATG CATATTGAATCACTGGACAGCTACACATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTC GAGACCTACGATGGTGGGAGCTGCGTCATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGT CTGCATGGTGGCACTGGTCCTGCTGCTCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAG CAGCTATCAAACCCCCCAAAGAACCCTTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACA GCAGCAGCGGGGCAGCTGTGGCTTCTTGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCC CAAGAATGGGAGTCTGCCTGCGGATGGTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTG CAGCTCGGGTCAGTGCATCTGACTTCATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAA GCTGCTGGATTCCACCATCAAGTGACAGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTA CAGACCTGTGGGGCCGGAGAAGCTGCATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCA GACCTGCCACGGCAGACAGAGCCAGTTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211795 representing BC017681
Red=Cloning site Green=Tags(s) MPWGRRPTWLLLAFLLVFLKISILSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMD IHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNS RVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSG EGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVT PGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECA TQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGR DGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGT DYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYW TDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNL LEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVN KRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCL PQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDG ETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGA HNCAENAACTNTEGGYNCTCAGRPSSPGRSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCM HIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLLLGMWGTYYYRTRK QLSNPPKNPCDEPSGSVSSSGPDSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSL QLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAP DLPRQTEPVQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC017681 |
| ORF Size | 3600 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC017681.1 |
| RefSeq Size | 4709 bp |
| RefSeq ORF | 3602 bp |
| Locus ID | 13645 |
| Gene Summary | This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201132 | Egf (untagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592), (10ug) |
CNY 8,360.00 |
|
| MR211795 | Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592) |
CNY 11,970.00 |
|
| MR211795L3 | Lenti ORF clone of Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592) |
CNY 13,870.00 |
|
| MR211795L4 | Lenti ORF clone of Egf (mGFP-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592) |
CNY 13,870.00 |
