Hdac6 (BC041105) Mouse Tagged ORF Clone

CAT#: MR211715

  • TrueORF®

Hdac6 (Myc-DDK-tagged) - Mouse histone deacetylase 6 (cDNA clone MGC:46887 IMAGE:5352032)

ORF Plasmid: DDK tGFP



  "BC041105" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 8,024.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-Hdac6 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Hdac5, mHDA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211715 representing BC041105
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTCCACCGGCCAAGATTCTTCTACTAGACAGCGAAAGAGTAGGCACAATCCCCAGTCACCCCTTC
AGGAATCCAGCGCCACCTTGAAGCGTGGTGGAAAGAAGTGTGCTGTACCCCACTCCAGCCCCAATCTAGC
GGAGGTAAAGAAGAAAGGCAAAATGAAGAAGCTGAGCCAACCAGCTGAAGAGGACCTAGTTGTGGGGCTT
CAAGGGCTGGATCTGAACCCTGAGACAAGAGTGCCAGTTGGTACTGGATTGGTGTTTGATGAACAACTAA
ATGACTTCCATTGCCTTTGGGATGACAGCTTCCCTGAAAGCCCTGAGCGGCTCCATGCCATCAGAGAGCA
ACTGATCCTGGAGGGCCTCCTGGGCCGCTGTGTGTCCTTTCAGGCCCGGTTCGCTGAGAAGGAGGAGCTG
ATGTTGGTTCACAGCCTGGAATACATTGATCTGATGGAGACAACCCAGTACATGAATGAAGGGGAGCTTC
GAGTACTGGCAGAAACCTATGATTCAGTGTATCTGCATCCGAACTCATATTCCTGTGCCTGCCTGGCTAC
AGGCTCTGTCCTCCGGCTGGTAGATGCACTCATGGGGGCTGAGATTCGGAATGGCATGGCCGTCATCAGG
CCTCCTGGACACCATGCTCAGCACAATCTTATGGATGGGTATTGCATGTTCAACCATCTGGCTGTGGCTG
CCCGCTATGCGCAAAAGAAGCACCGCATTCAGAGGGTTCTCATCGTGGACTGGGATGTGCACCATGGTCA
AGGAACACAGTTCATCTTCGACCAGGACCCCAGTGTCCTTTATTTCTCCATCCACCGATATGAACATGGT
CGCTTCTGGCCCCACCTTAAGGCTTCTAACTGGTCCACTATAGGTTTTGGCCAAGGCCAAGGATATACCA
TCAATGTACCTTGGAACCAGACGGGCATGCGGGACGCTGACTACATTGCTGCTTTCCTGCACATCCTGCT
GCCAGTTGCCTCGGAGTTTCAGCCTCAACTGGTCTTGGTGGCCGCTGGATTTGATGCCCTCCACGGAGAC
CCCAAGTCCCTTCAGGGAGAGATGGCTGCCACGCCAGCAGGATTTGCCCACCTAACCCATTTGCTCATGG
GTTTGGCAGGAGGCAAGTTGATTCTGTCCCTGGAGGGTGGCTATAACCTCCGTGCCCTGGCTAAGGGAGT
CAGTGCTTCACTCCACACCCTTCTTGGAGACCCTTGCCCCATGCTGGAGTCCTGTGTTGTACCTTGTGCA
AGCGCCCAGACTTCCATCTACTGCACTCTAGAAGCCCTTGAACCCTTCTGGGAGGTCCTGGAGAGATCAG
TTGAGACCCAGGAGGAAGATGAAGTGGAAGAAGCCGTGCTAGAAGAGGAGGAGGAGGAAGGTGGCTGGGA
GGCCACTGCACTGCCCATGGATACATGGCCACTGCTCCAGAACCGCACTGGGCTGGTCTATGATGAGAAG
ATGATGAGTCACTGCAACCTCTGGGACAATCATCACCCTGAGACACCTCAGCGCATCTTACGCATCATGT
GTCACCTGGAGGAGGTGGGCCTTGCGGCTCGCTGTCTCATCCTACCTGCTCGGCCTGCCCTGGACTCTGA
GCTCCTTACCTGCCACAGTGCTGAGTACGTGGAGCATCTCCGCACCACAGAAAAGATGAAAACCCGGGAT
CTGCACCGTGAAGGTGCCAACTTTGACTCCATCTACATCTGCCCCAGCACCTTTGCCTGCGCAAAGCTTG
CCACAGGCGCTGCCTGCCGCCTGGTGGAAGCTGTGCTCTCGGGAGAGGTTCTAAATGGCATTGCAGTAGT
GCGCCCTCCAGGACACCACGCGGAGCCAAATGCTGCCTGTGGTTTCTGCTTTTTCAACTCAGTAGCTGTA
GCTGCTCGCCATGCCCAGATCATTGCTGGACGTGCCCTGCGGATCCTAATCGTGGATTGGGATGTTCATC
ATGGTAATGGAACTCAGCACATATTTGAGGATGACCCTAGTGTATTATACGTGTCCCTGCACCGGTATGA
CCGTGGCACTTTCTTCCCCATGGGGGATGAGGGTGCCAGTAGCCAAGTAGGCCGAGATGCAGGTATAGGC
TTCACTGTCAATGTGCCCTGGAATGGGCCCCGCATGGGTGATGCTGATTACCTGGCTGCATGGCATCGTC
TGGTACTTCCCATCGCCTATGAGTTTAACCCAGAACTGGTGCTGATCTCAGCTGGCTTTGATGCTGCACA
AGGGGATCCGCTGGGGGGCTGCCAAGTAACACCGGAGGGTTATGCCCACCTCACCCACCTACTGATGGGC
CTTGCTGGTGGCCGTATTATTCTTATTCTAGAGGGTGGATACAATTTGGCATCTATCTCTGAGTCTATGG
CTGCCTGCACCCATTCCCTCCTTGGAGACCCACCACCCCAGCTTACTTTGCTGCGACCGCCACAGTCAGG
AGCCCTGGTTTCAATCAGTGAGGTCATCCAAGTCCATCGCAAATACTGGCGCAGTTTGCGGTTGATGAAA
ATGGAAGACAAGGAAGAATGCTCTAGTTCTAGGCTTGTCATCAAGAAGTTGCCCCCAACAGCCAGTCCTG
TATCAGCTAAGGAAATGACCACACCGAAAGGAAAGGTTCCTGAAGAAAGCGTGAGGAAGACCATAGCAGC
ACTACCTGGGAAAGAGTCTACTCTAGGCCAGGCTAAATCAAAGATGGCTAAGGCTGTGCTTGCTCAGGGC
CAGTCCTCAGAACAAGCTGCTAAGGGAACTACACTGGATCTGGCTACCTCAAAGGAGACTGTGGGAGGAG
CCACGACGGACCTGTGGGCCTCAGCAGCTGCTCCTGAAAACTTCCCTAACCAGACCACCTCTGTGGAGGC
TTTGGGAGAAACTGAGCCAACGCCTCCAGCCTCGCATACAAACAAGCAGACCACAGGGGCTTCACCTCTG
CAGGGAGTCACGGCTCAGCAGTCCCTACAGCTTGGGGTTCTCAGCACTTTGGAGCTAAGCAGAGAAGCAG
AGGAAGCCCATGATTCTGAGGAGGGCCTGCTAGGGGAAGCCGCTGGAGGTCAGGACATGAACAGCTTGAT
GCTGACACAAGGATTTGGGGACTTTAATACCCAGGATGTATTTTATGCTGTGACCCCACTATCCTGGTGT
CCCCATTTGATGGCAGTATGCCCCATTCCTGCAGCAGGCCTAGATGTGTCCCAACCTTGTAAGACCTGTG
GAACAGTCCAGGAGAACTGGGTGTGTCTGACTTGCTATCAGGTGTACTGCAGTCGCTATGTCAATGCCCA
TATGGTCTGCCACCATGAAGCCTCTGAACACCCGCTGGTCCTCAGCTGTGTTGACCTGTCTACCTGGTGT
TATGTCTGTCAGGCTTATGTCCACCACGAGGATCTCCAAGATGTGAAGAACGCTGCCCACCAGAACAAGT
TTGGGGAGGACATGCCCCACTCACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211715 representing BC041105
Red=Cloning site Green=Tags(s)

MTSTGQDSSTRQRKSRHNPQSPLQESSATLKRGGKKCAVPHSSPNLAEVKKKGKMKKLSQPAEEDLVVGL
QGLDLNPETRVPVGTGLVFDEQLNDFHCLWDDSFPESPERLHAIREQLILEGLLGRCVSFQARFAEKEEL
MLVHSLEYIDLMETTQYMNEGELRVLAETYDSVYLHPNSYSCACLATGSVLRLVDALMGAEIRNGMAVIR
PPGHHAQHNLMDGYCMFNHLAVAARYAQKKHRIQRVLIVDWDVHHGQGTQFIFDQDPSVLYFSIHRYEHG
RFWPHLKASNWSTIGFGQGQGYTINVPWNQTGMRDADYIAAFLHILLPVASEFQPQLVLVAAGFDALHGD
PKSLQGEMAATPAGFAHLTHLLMGLAGGKLILSLEGGYNLRALAKGVSASLHTLLGDPCPMLESCVVPCA
SAQTSIYCTLEALEPFWEVLERSVETQEEDEVEEAVLEEEEEEGGWEATALPMDTWPLLQNRTGLVYDEK
MMSHCNLWDNHHPETPQRILRIMCHLEEVGLAARCLILPARPALDSELLTCHSAEYVEHLRTTEKMKTRD
LHREGANFDSIYICPSTFACAKLATGAACRLVEAVLSGEVLNGIAVVRPPGHHAEPNAACGFCFFNSVAV
AARHAQIIAGRALRILIVDWDVHHGNGTQHIFEDDPSVLYVSLHRYDRGTFFPMGDEGASSQVGRDAGIG
FTVNVPWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLISAGFDAAQGDPLGGCQVTPEGYAHLTHLLMG
LAGGRIILILEGGYNLASISESMAACTHSLLGDPPPQLTLLRPPQSGALVSISEVIQVHRKYWRSLRLMK
MEDKEECSSSRLVIKKLPPTASPVSAKEMTTPKGKVPEESVRKTIAALPGKESTLGQAKSKMAKAVLAQG
QSSEQAAKGTTLDLATSKETVGGATTDLWASAAAPENFPNQTTSVEALGETEPTPPASHTNKQTTGASPL
QGVTAQQSLQLGVLSTLELSREAEEAHDSEEGLLGEAAGGQDMNSLMLTQGFGDFNTQDVFYAVTPLSWC
PHLMAVCPIPAAGLDVSQPCKTCGTVQENWVCLTCYQVYCSRYVNAHMVCHHEASEHPLVLSCVDLSTWC
YVCQAYVHHEDLQDVKNAAHQNKFGEDMPHSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC041105
ORF Size 3456 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC041105.1
RefSeq Size 4016 bp
RefSeq ORF 3458 bp
Locus ID 15185
MW 147.2 kDa
Gene Summary Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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