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Tsc1 (BC052399) Mouse Tagged ORF Clone
CAT＃: MR211709
- TrueORF®
Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967)
ORF Plasmid: tGFP
"BC052399" in other vectors (4)
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CNY 11,500.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hamartin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211709 representing BC052399
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGTTAGCCAACATTGGGGAGCTGCTCTCCATGCTGGACTCCTCCACACTGGGTGTGCGGGATG ACGTGACAGCCATCTTCAAGGAGTCCCTCAATTCTGAACGTGGGCCTATGCTTGTCAACACGTTGGTTGA TTATTACCTGGAAACCAATTCTCAGCCGGTATTGCACATCCTGACCACCTTGCAGGAGCCACACGATAAG CACCTCTTGGACAAAATTAATGAGTATGTAGGCAAAGCTGCTACCCGTTTATCCATCCTCTCGCTGCTGG GGCATGTTGTGAGACTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTCTGCCTTCTTTATT GAAATGTCTCAAGATGGACACTGATGTTGTGGTCCTCACAACTGGTGTCTTGGTGTTGATCACCATGCTC CCGATGATCCCGCAGTCAGGGAAGCAGCACCTTCTCGACTTCTTTGACATCTTTGGCCGTCTCTCGTCAT GGTGCCTGAAGAAACCAGGCCATGTGACAGAAGTGTACCTGGTCCATCTTCATGCCAGTGTTTATGCCCT CTTTCACCGCCTTTATGGGATGTACCCATGTAACTTCGTCTCCTTCCTGCGCTCTCACTACAGTATGAAG GAAAACGTGGAGACTTTTGAAGAAGTGGTCAAGCCAATGATGGAGCATGTGCGAATTCACCCGGAATTAG TGACTGGATCCAAGGACCATGAACTGGACCCTCGAAGGTGGAAGACATTAGAAACTCATGATGTTGTAAT AGAGTGTGCCAAAATCTCTCTGGACCCTACAGAAGCCTCGTATGAAGATGGCTATTCTGTGTCACACCAG CTCTCTGCTTGCTTCCCTTACCGTTCAGCTGATGTCACCACCAGCCCTTATGTGGACACACAGAATAGCT ATGGGGGTTCTACTTCCACCCCTTCCTCCAGCTCTCGGCTGATGTTGTTCAGTCCACCTGGGCAGCTACC TCAGAGTTTGAGTTCCCCATCAACACGGCTGTTACCTGAGCCGCTGCAAGCTAGTCTCTGGAGCCCATCT GCGGTCTGTGGTATGACCACTCCTCCTACGTCTCCTGGAAATGTCCCAGCTGATTTGTCACATCCGTATA GTAAAGCCTTTGGTACCACTGGTGGAAAAGGAACTCCTTCAGGAACCCCAGCGACCTCTCCTCCTCCAGC CCCACCTTGTCCCCAAGATGACTGTGTGCATGGTTCAGCAGCCCAGGCCTCAGCCACAGCCCCCAGGAAG GAAGAAAGAGCAGATTCCTCAAGGCCTTACCTTCACAGACAGTCAAACGACCGAGGATTAGAGGATCCAC CTGGAAGCAAAGGTTCCGTTACTCTGAGGAATCTACCTGATTTCCTAGGTGATCTGGCTTCTGAGGAAGA CAGTATCGAGAAAGATAAGGAAGAAGCTGCAATATCTAAAGAGCTTTCTGAGATCACTACTGCAGAGGCG GATCCTGTAGTTCCTCGAGGGGGCTTTGACTCTCCCTTCTACCGAGACAGTCTCTCTGGCTCTCAGCGGA AGACTCATTCGGCAGCCTCTGGGACTCAGGGCTCCAGCGTGAACCCTGAGCCTTTGCACTCCTCCCTGGA CAAACATGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCCACCCTCTGGCAGTGCTGATGTC AGTCCCGCTGGGGACAGGGATCGCCAGACTTCTCTGGAGACCAGTATCCTCACTCCCAGCCCTTGCAAAA TCCCACCTCAGAGGGGAGTGAGCTTTGGAAGTGGGCAGCTTCCCCCATATGATCATCTCTTTGAGGTGGC CTTGCCAAAGACTGCCTGTCACTTTGTCAGCAAGAAGACTGAGGAGCTGTTGAAGAAAGTGAAAGGAAAC CCTGAGGAAGACTGTGTGCCCTCTACCTCCCCAATGGAAGTACTGGACAGACTGATAGAGCAGGGAGCAG GTGCGCACAGCAAGGAGCTGAGCAGGTTGTCCCTGCCCAGCAAGTCTGTTGACTGGACCCACTTTGGAGG CTCTCCTCCCTCAGATGAGCTCCGGACCCTCCGAGACCAGTTGCTTTTACTGCACAATCAGCTGCTGTAC GAGCGCTTCAAGCGGCAGCAGCATGCACTCAGGAACAGAAGGCTCCTGCGCAAGGTGATCCGAGCAGCGG CTCTGGAGGAACACAATGCAGCAATGAAAGATCAGTTGAAGTTACAAGAGAAGGACATACAGATGTGGAA GGTGAGTCTGCAGAAAGAACAAGCCCGATACAGTCAGCTTCAGGAACAACGTGACACCATGGTGACCCAA CTGCATAGCCAGATCAGACAGCTACAGCATGACCGAGAAGAATTCTACAACCAGAGTCAGGAGTTACAGA CAAAGCTGGAGGACTGCAGAAACATGATTGCGGAGCTTCGGGTGGAGCTGAAGAAGGCTAACAACAAGGT GTGCCACACTGAGCTGCTGCTCAGCCAGGTCTCTCAGAAGCTCTCCAACAGTGAGTCAGTGCAGCAGCAG ATGGAGTTCTTGAATAGGCAGCTCCTGGTGCTCGGGGAGGTCAATGAGCTGTACCTGGAGCAGCTGCAGA GCAAGCATCCTGACACCACCAAGGAAGTAGAAATGATGAAAACTGCATATCGGAAAGAGCTAGAGAAAAA CAGAAGCCACCTTCTCCAGCAGAACCAAAGGTTGGACGCCTCACAGAGGCGAGTTTTGGAACTGGAGTCT CTTCTGGCCAAGAAAGACCACCTTCTCCTAGAACAGAAGAAATATCTTGAGGATGTCAAGAGCCAGGCGA GTGGACAGCTGCTGGCTGCAGAGAGCAGGTATGAGGCTCAGAGAAAGATCACCCGGGTGTTGGAACTGGA GATCCTAGACTTGTATGGCAGGTTGGAAAAAGATGGCCGCCTACGGAAACTAGAAGAGGACAGAGCAGAG GCAGCAGAGGCAGCAGAAGAGAGGCTTGACTGTTGTAGTGATGGATGCACAGATTCCTTGGTAGGACATA ATGAAGAGGCTTCTGGTCACAATGGTGAGACCAGGACCTCCAGACCTGGTGGCACCCGGGCCAGCTGTGG AGGTAGAGTCACTGGAGGCAGCAGCAGCAGCAGCAGTGAGCTTTCCACTCCAGAGAAACCCCCGAGCCAG AGGTTCAGCAGCATCCCCACCACTGTTGGCTCACTTCCCAGTTCCAAAAGCTTCCTGGGCATGAAGGCCC GGGAGCTGTTCCGTAATAAGAGCGAGAGCCAGTGTGATGAGGACAGCGTGACCATGAGTAGCAGCAGCCT TTCTGAGACCCTGAAGACAGAACTGGGCAAGGACTCGGGCACAGAAAACAAGACTTCCCTGAGTCTAGAT GCCCCACACCCATCTTCCCCAAACTCAGACAATGTGGGGCAGCTCCACATCATGGACTACAATGAGACTC ATCCTGAACACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211709 representing BC052399
Red=Cloning site Green=Tags(s) MAQLANIGELLSMLDSSTLGVRDDVTAIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDK HLLDKINEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITML PMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMK ENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGYSVSHQ LSACFPYRSADVTTSPYVDTQNSYGGSTSTPSSSSRLMLFSPPGQLPQSLSSPSTRLLPEPLQASLWSPS AVCGMTTPPTSPGNVPADLSHPYSKAFGTTGGKGTPSGTPATSPPPAPPCPQDDCVHGSAAQASATAPRK EERADSSRPYLHRQSNDRGLEDPPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEA DPVVPRGGFDSPFYRDSLSGSQRKTHSAASGTQGSSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADV SPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKVKGN PEEDCVPSTSPMEVLDRLIEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDELRTLRDQLLLLHNQLLY ERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQEQRDTMVTQ LHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANNKVCHTELLLSQVSQKLSNSESVQQQ MEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELES LLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLRKLEEDRAE AAEAAEERLDCCSDGCTDSLVGHNEEASGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPSQ RFSSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTMSSSSLSETLKTELGKDSGTENKTSLSLD APHPSSPNSDNVGQLHIMDYNETHPEHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052399 |
| ORF Size | 3444 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052399.1 |
| RefSeq Size | 4829 bp |
| RefSeq ORF | 3446 bp |
| Locus ID | 64930 |
| MW | 177 kDa |
| Gene Summary | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed:16707451). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (PubMed:29127155). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (PubMed:29127155). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223091 | Tsc1 (untagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967), (10ug) |
CNY 12,920.00 |
|
| MG211709 | Tsc1 (tGFP-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967) |
CNY 12,640.00 |
|
| MR211709L3 | Lenti ORF clone of Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967) |
CNY 13,400.00 |
|
| MR211709L4 | Lenti ORF clone of Tsc1 (mGFP-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967) |
CNY 13,400.00 |
