Abl1 (BC059260) Mouse Tagged ORF Clone

CAT#: MR211702

  • TrueORF®

Abl1 (Myc-DDK-tagged) - Mouse v-abl Abelson murine leukemia oncogene 1 (cDNA clone MGC:67604 IMAGE:6408990)

ORF Plasmid: DDK tGFP



  "BC059260" in other vectors (3)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 7,952.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Abl, c-Abl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211702 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGCAGCCTGGAAAAGTTCTTGGGGACCAAAGAAGGCCTAGTTTGCCCGCCCTGCATTTTATCA
AAGGGGCAGGGAAGAGGGACTCATCGAGGCATGGGGGCCCACACTGCAATGTCTTTGTGGAACACGAAGC
CCTGCAGAGGCCAGTGGCATCTGACTTTGAGCCCCAGGGTCTCAGCGAAGCAGCTCGATGGAACTCCAAG
GAAAACCTTCTTGCTGGGCCCAGTGAAAATGACCCCAACCTTTTTGTGGCACTCTATGATTTTGTGGCCA
GTGGAGATAACACTCTCAGCATCACTAAAGGTGAAAAGCTCCGGGTCTTGGGTTATAATCACAATGGGGA
ATGGTGTGAAGCCCAAACGAAAAATGGCCAAGGATGGGTCCCAAGCAACTACATCACCCCCGTCAACAGC
CTGGAGAAACATTCCTGGTATCATGGCCCTGTATCTCGGAATGCTGCTGAGTATCTGCTGAGCAGCGGAA
TCAACGGCAGCTTCTTAGTGCGGGAGAGTGAGAGTAGCCCTGGCCAGAGATCCATCTCGCTGCGGTATGA
AGGGAGGGTGTACCACTACAGGATCAACACTGCCTCTGATGGCAAGCTGTACGTGTCCTCCGAGAGCCGC
TTCAACACTCTGGCTGAGTTAGTTCACCATCACTCCACGGTGGCTGATGGCCTCATCACCACACTCCACT
ACCCAGCTCCCAAGCGCAACAAGCCCACTATCTACGGTGTGTCCCCCAACTACGACAAGTGGGAAATGGA
GCGCACCGACATCACCATGAAGCACAAGTTGGGTGGAGGCCAGTACGGGGAGGTGTACGAGGGCGTTTGG
AAGAAGTACAGCCTCACTGTGGCCGTGAAGACCTTGAAGGAGGACACCATGGAGGTGGAGGAGTTCCTGA
AGGAAGCGGCGGTGATGAAGGAGATCAAACACCCTAACCTGGTGCAGCTGCTAGGGGTGTGTACCCGGGA
ACCACCATTCTACATAATCACTGAGTTCATGACCTATGGGAACCTGCTGGACTACCTGAGGGAGTGTAAC
CGGCAGGAGGTGAGCGCCGTGGTACTGCTCTACATGGCCACACAGATCTCATCAGCCATGGAGTACTTGG
AGAAGAAGAACTTCATCCACAGAGACCTTGCTGCCCGGAACTGCCTGGTAGGGGAAAACCACTTGGTGAA
GGTGGCTGATTTTGGCCTGAGCAGGTTGATGACAGGGGACACCTACACGGCCCATGCTGGAGCCAAATTC
CCCATCAAATGGACCGCACCTGAGAGCCTGGCCTACAACAAGTTCTCCATCAAGTCGGACGTGTGGGCAT
TTGGAGTATTGCTCTGGGAGATTGCTACCTATGGCATGTCACCTTACCCGGGAATTGACCTGTCTCAGGT
TTATGAGCTGCTGGAAAAAGACTACCGCATGGAGCGCCCTGAAGGCTGCCCGGAGAAGGTCTACGAGCTC
ATGCGAGCATGTTGGCAGTGGAACCCCTCTGACCGGCCCTCCTTTGCTGAAATCCACCAAGCCTTTGAAA
CCATGTTCCAGGAATCCAGTATCTCAGATGAGGTGGAGAAGGAGCTGGGGAAACGAGGCACGAGAGGAGG
TGCTGGGAGTATGCTGCAGGCCCCAGAGCTGCCCACCAAGACCAGAACCTGCAGGAGAGCAGCTGAGCAG
AAAGATGCGCCTGACACCCCTGAGCTGCTCCACACGAAGGGCCTGGGAGAAAGCGATGCACTGGACAGTG
AGCCTGCTGTATCGCCACTGCTTCCTCGGAAAGAGCGCGGGCCCCCAGACGGCAGCCTAAATGAAGATGA
GCGCCTTCTCCCCAGAGACAGAAAGACCAACCTGTTCAGCGCTTTGATCAAGAAGAAGAAGAAAATGGCG
CCGACGCCCCCTAAGCGCAGCAGTTCCTTCCGAGAGATGGATGGCCAGCCAGACCGCAGAGGGGCTAGTG
AGGATGACAGCAGGGAACTCTGCAATGGACCACCAGCTCTCACCTCAGACGCAGCAGAGCCTACCAAGTC
CCCAAAGGCCAGCAATGGGGCTGGCGTCCCTAATGGAGCCTTCCGGGAGCCGGGCAACTCAGGCTTCCGT
TCTCCCCACATGTGGAAAAAGTCCAGCACACTGACCGGGAGCCGCCTGGCTGCTGCCGAAGAGGAGAGCG
GCATGAGCTCCAGTAAGCGCTTCCTGCGTTCTTGTTCGGCCTCCTGCATGCCCCATGGGGCAAGGGACAC
AGAGTGGCGGTCGGTCACGCTGCCTCGAGACCTGCCGTCTGCTGGCAAGCAGTTTGACTCATCCACCTTT
GGAGGGCACAAAAGCGAAAAGCCAGCTCTGCCTCGGAAACGCACCAGTGAGAGCAGGTCTGAGCAGGTGG
CCAAAAGCACGGCGATGCCCCCTCCCCGGCTGGTGAAGAAGAACGAGGAGGCTGCTGAAGAAGGCTTCAA
AGACACAGAATCCAGCCCTGGCTCCAGCCCTCCCAGCTTGACTCCCAAACTCCTCCGCAGGCAGGTCACT
GCCTCTCCTTCCTCTGGCCTCTCTCACAAGGAAGAGGCCACCAAGGGCAGTGCCTCAGGCATGGGGACTC
CGGCCACTGCAGAGCCAGCACCCCCCAGCAACAAAGTGGGCCTCAGCAAGGCCTCCTCTGAGGAGATGCG
CGTAAGGAGGCACAAGCACAGCTCGGAGTCCCCAGGGAGAGACAAGGGGCGACTGGCTAAGCTCAAGCCT
GCCCCGCCGCCTCCTCCTGCCTGCACAGGAAAAGCAGGCAAGCCCGCACAGAGCCCCAGCCAAGAGGCCG
GGGAGGCAGGGGGGCCCACAAAGACAAAATGCACGAGTCTGGCTATGGATGCTGTGAACACTGACCCCAC
CAAGGCCGGCCCACCTGGAGAAGGACTGAGAAAGCCTGTGCCCCCATCTGTGCCAAAGCCCCAGTCGACG
GCTAAGCCTCCAGGGACTCCCACCAGCCCGGTCTCCACCCCCTCCACAGCACCAGCTCCTTCACCCCTGG
CTGGGGACCAGCAGCCATCTTCTGCCGCCTTCATCCCCCTCATATCAACCCGTGTGTCTCTTAGGAAGAC
CCGCCAGCCGCCAGAGCGCATTGCCAGTGGCACCATCACCAAGGGTGTGGTTCTGGACAGTACTGAGGCC
CTGTGCCTTGCCATCTCCCGGAACTCAGAGCAGATGGCCAGCCACAGTGCTGTACTGGAGGCTGGCAAGA
ACCTGTACACTTTCTGTGTGAGCTATGTGGACTCTATCCAGCAGATGAGGAACAAGTTTGCCTTCCGTGA
GGCTATCAACAAGCTGGAGAGCAACCTCCGAGAGCTGCAGATCTGCCCTGCCACAGCCTCCAGTGGGCCA
GCTGCCACCCAAGACTTCAGCAAGCTGCTCAGCTCTGTGAAGGAGATCAGCGACATTGTCCGGAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211702 protein sequence
Red=Cloning site Green=Tags(s)

MGQQPGKVLGDQRRPSLPALHFIKGAGKRDSSRHGGPHCNVFVEHEALQRPVASDFEPQGLSEAARWNSK
ENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNS
LEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESR
FNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVW
KKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECN
RQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKF
PIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYEL
MRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQ
KDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMA
PTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFR
SPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSSTF
GGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDTESSPGSSPPSLTPKLLRRQVT
ASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKP
APPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQST
AKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEA
LCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGP
AATQDFSKLLSSVKEISDIVRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC059260
ORF Size 3426 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC059260, AAH59260
RefSeq Size 5168 bp
RefSeq ORF 3428 bp
Locus ID 11350
MW 124.8 kDa
Gene Summary Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (By similarity). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-191' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. Regulates T-cell differentiation in a TBX21-dependent manner (PubMed:21690296). Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (PubMed:21690296).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...