Grip1 (BC072632) Mouse Tagged ORF Clone

CAT#: MR211652

  • TrueORF®

Grip1 (Myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:86044 IMAGE:6843802)

ORF Plasmid: DDK tGFP



  "BC072632" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,752.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 4931400F03Rik; eb; GRIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211652 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGCTGGAAGAAGAACATCCCCATCTGCTTACAAGCGGAGGAGCAGGAGAGAGATGAGAGTCCCT
ACACTAAATCTGCCAGCCAGACAAAGCCGCCCGATGGAGCATTGGCTGTGAGGAGACAGAGCATCCCAGA
GGAATTCAAGGGCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCG
GGAGGCATTGATAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTG
ACCAGCTGGATGTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGA
GATCATCAGCCTGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTC
TCTGTACAAGGATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTG
GTTTTGTCATCCGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCG
TCCTGGAGGGCCTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATT
CGGCTCCTGGGAACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGC
TGATAGAATACGATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGC
CAAAACTCCAGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTC
ATAGACAAAATCAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCT
CCATTGACGGCACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGA
CCAGGTCAAGCTGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGTGAAA
ATTCAGAGGAGCGACAGACAACATCCCTGGGATGCCTGGGCCAGCAACCAGTGCGGCGTTCATACCAACC
ATCACCATAACACGTACCACCCAGACCATTGCAGAGTGCCAGCCCTGACTTTCCCGAAAGCGCTTCCTCC
AAACAGCCCTCCGGCTATGGTGCCCTCATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTG
AACATGGGGACTTTACCTCGAAGCCTCTACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGA
AAAAGAAAGACTTCAAAAGCTCACTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCA
CACTGAAACCACAGAGGTTGTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGC
GTGTTTGCCACAGAGACGCTCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGA
GATGTGGTGTGCTACAGATTGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTT
CGAGGAAGCCAATCAACTCCTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGAT
GTTGCAGAGTCTGTCATCCCAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAAC
TTGGAATAACCATCAGTTCGCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAA
AGGCAGTGTGGCACACAGAACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGG
CTGGATAACTGTTCCATGGAAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAA
TCCGCAAAGATGAAGATAACTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAA
GCGCTATGGGGGGCCCCTTGGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCG
AGCCTCACTAAAGGGGGATTAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCA
ATAGCAGCAGCTTGAAGGGGAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGT
CACCCTGAAAATTAAGAAACAGACAGATGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGC
CACTCGGGGGACCTAGGAGATGGTGAGGAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATG
CGTACCCCTCCACGGTGCCCAGCGTGGACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAG
CTATGGGAGTCAAGGCTCAACTTTTCAGACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCA
AAGCAAAGAACCAGCCTGTCCCCAGTCCCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTA
ATGAAGACTGGGATCGATCCACAGCCAGTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGA
GGAAAACTTCTGGTCTCAAGCATTGGAGGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTT
GAGGCAACAATCATGTCGGGGAGTACTATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGG
GGCGACAGGCCAGCTTCCAGGAACGGAGCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACAC
CCTGCCCTCAGACGTGGGCAGAAAGTCTGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATG
TCCCCAACTCCGGTGGAGCTACACAAGGTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCA
GTGTGGCAGATGGCCTGCTGGAGAAAGGCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGT
TGGGGGCTTGAAGCCCTACGACAGGCTCTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGC
CTGGTGGTGCCTCTCATAGCTGAATCTGGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCT
CCCAGAAGTCGATAGAACAGCCGGCTCTGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCA
ACCCAGCCACGGGATTCCTGGAGATACTGTATACTTTTGGCAAAGCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211652 protein sequence
Red=Cloning site Green=Tags(s)

MPGWKKNIPICLQAEEQERDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGTTLGLTVS
GGIDKDGKPRVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPV
SVQGSSVMFRTVEVTLHKEGNTFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGI
RLLGTTHAEAMSILKQCGQEATLLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIV
IDKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHVK
IQRSDRQHPWDAWASNQCGVHTNHHHNTYHPDHCRVPALTFPKALPPNSPPAMVPSSSPTSMSAYSLSSL
NMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGS
VFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQLLRDSSITSKVTLEIEFD
VAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIR
LDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPLGITISGTEEPFDPIIIS
SLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKKQTDAQSASSPKKFPIPG
HSGDLGDGEEDPSPIQKPGKLSDAYPSTVPSVDSAVDSWDGSGIDASYGSQGSTFQTSGYNYNTYDWRSP
KQRTSLSPVPKPRSQTYPDVGLSNEDWDRSTASGFVGASDSADAEQEENFWSQALEDLETCGQSGILREL
EATIMSGSTMSLNHEAPMARSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIM
SPTPVELHKVTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDVGGLKPYDRLLQVNHVRTRDFDCC
LVVPLIAESGNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGIPGDTVYFWQSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC072632
ORF Size 3339 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC072632, AAH72632
RefSeq Size 4064 bp
RefSeq ORF 3341 bp
Locus ID 74053
MW 120.5 kDa
Gene Summary This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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