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Grip1 (BC072632) Mouse Tagged ORF Clone
CAT＃: MR211652
- TrueORF®
Grip1 (Myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:86044 IMAGE:6843802)
ORF Plasmid: tGFP
"BC072632" in other vectors (4)
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CNY 7,752.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4931400F03Rik; eb; GRIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211652 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGCTGGAAGAAGAACATCCCCATCTGCTTACAAGCGGAGGAGCAGGAGAGAGATGAGAGTCCCT ACACTAAATCTGCCAGCCAGACAAAGCCGCCCGATGGAGCATTGGCTGTGAGGAGACAGAGCATCCCAGA GGAATTCAAGGGCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCG GGAGGCATTGATAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTG ACCAGCTGGATGTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGA GATCATCAGCCTGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTC TCTGTACAAGGATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTG GTTTTGTCATCCGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCG TCCTGGAGGGCCTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATT CGGCTCCTGGGAACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGC TGATAGAATACGATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGC CAAAACTCCAGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTC ATAGACAAAATCAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCT CCATTGACGGCACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGA CCAGGTCAAGCTGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGTGAAA ATTCAGAGGAGCGACAGACAACATCCCTGGGATGCCTGGGCCAGCAACCAGTGCGGCGTTCATACCAACC ATCACCATAACACGTACCACCCAGACCATTGCAGAGTGCCAGCCCTGACTTTCCCGAAAGCGCTTCCTCC AAACAGCCCTCCGGCTATGGTGCCCTCATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTG AACATGGGGACTTTACCTCGAAGCCTCTACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGA AAAAGAAAGACTTCAAAAGCTCACTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCA CACTGAAACCACAGAGGTTGTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGC GTGTTTGCCACAGAGACGCTCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGA GATGTGGTGTGCTACAGATTGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTT CGAGGAAGCCAATCAACTCCTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGAT GTTGCAGAGTCTGTCATCCCAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAAC TTGGAATAACCATCAGTTCGCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAA AGGCAGTGTGGCACACAGAACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGG CTGGATAACTGTTCCATGGAAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAA TCCGCAAAGATGAAGATAACTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAA GCGCTATGGGGGGCCCCTTGGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCG AGCCTCACTAAAGGGGGATTAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCA ATAGCAGCAGCTTGAAGGGGAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGT CACCCTGAAAATTAAGAAACAGACAGATGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGC CACTCGGGGGACCTAGGAGATGGTGAGGAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATG CGTACCCCTCCACGGTGCCCAGCGTGGACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAG CTATGGGAGTCAAGGCTCAACTTTTCAGACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCA AAGCAAAGAACCAGCCTGTCCCCAGTCCCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTA ATGAAGACTGGGATCGATCCACAGCCAGTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGA GGAAAACTTCTGGTCTCAAGCATTGGAGGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTT GAGGCAACAATCATGTCGGGGAGTACTATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGG GGCGACAGGCCAGCTTCCAGGAACGGAGCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACAC CCTGCCCTCAGACGTGGGCAGAAAGTCTGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATG TCCCCAACTCCGGTGGAGCTACACAAGGTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCA GTGTGGCAGATGGCCTGCTGGAGAAAGGCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGT TGGGGGCTTGAAGCCCTACGACAGGCTCTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGC CTGGTGGTGCCTCTCATAGCTGAATCTGGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCT CCCAGAAGTCGATAGAACAGCCGGCTCTGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCA ACCCAGCCACGGGATTCCTGGAGATACTGTATACTTTTGGCAAAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211652 protein sequence
Red=Cloning site Green=Tags(s) MPGWKKNIPICLQAEEQERDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGTTLGLTVS GGIDKDGKPRVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPV SVQGSSVMFRTVEVTLHKEGNTFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGI RLLGTTHAEAMSILKQCGQEATLLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIV IDKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHVK IQRSDRQHPWDAWASNQCGVHTNHHHNTYHPDHCRVPALTFPKALPPNSPPAMVPSSSPTSMSAYSLSSL NMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGS VFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQLLRDSSITSKVTLEIEFD VAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIR LDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPLGITISGTEEPFDPIIIS SLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKKQTDAQSASSPKKFPIPG HSGDLGDGEEDPSPIQKPGKLSDAYPSTVPSVDSAVDSWDGSGIDASYGSQGSTFQTSGYNYNTYDWRSP KQRTSLSPVPKPRSQTYPDVGLSNEDWDRSTASGFVGASDSADAEQEENFWSQALEDLETCGQSGILREL EATIMSGSTMSLNHEAPMARSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIM SPTPVELHKVTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDVGGLKPYDRLLQVNHVRTRDFDCC LVVPLIAESGNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGIPGDTVYFWQSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC072632 |
| ORF Size | 3339 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC072632, AAH72632 |
| RefSeq Size | 4064 bp |
| RefSeq ORF | 3341 bp |
| Locus ID | 74053 |
| MW | 120.5 kDa |
| Gene Summary | This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223102 | Grip1 (untagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:86044 IMAGE:6843802), (10ug) |
CNY 12,920.00 |
|
| MG211652 | Grip1 (tGFP-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:86044 IMAGE:6843802) |
CNY 7,030.00 |
|
| MR211652L3 | Lenti ORF clone of Grip1 (Myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:86044 IMAGE:6843802) |
CNY 8,270.00 |
|
| MR211652L4 | Lenti ORF clone of Grip1 (mGFP-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:86044 IMAGE:6843802) |
CNY 8,270.00 |
