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Ap3b1 (NM_009680) Mouse Tagged ORF Clone
CAT＃: MR211641
- TrueORF®
Ap3b1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, beta 1 subunit (Ap3b1)
ORF Plasmid: tGFP
"NM_009680" in other vectors (4)
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CNY 7,696.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AP-3; AU015684; beta3A; C78395; Hps2; pe; pearl; rim2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211641 representing NM_009680
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTAGCAACAGTTTCGCCTACAACGAGCAGTCGGGAGGAGGGGAGGCGGCGGAGCTCGGGCAGGAGG CCACCTCGACCATCTCTCCCTCGGGCGCCTTCGGCCTCTTCAGCAGCGATTGGAAGAAGAATGAAGACCT GAAGCAGATGCTGGAGAGCAACAAAGATTCTGCCAAGCTAGATGCTATGAAGCGAATTGTTGGGATGATT GCGAAGGGGAAAAATGCCTCGGAACTCTTCCCTGCTGTTGTGAAGAATGTGGCCAGTAAAAACATCGAGA TCAAGAAGTTGGTGTACGTTTACCTTGTCCGATATGCTGAAGAACAGCAGGACCTGGCTCTTCTGTCCAT AAGCACTTTCCAGCGAGCTTTGAAGGACCCGAATCAACTAATCCGTGCAAGTGCCTTGAGAGTTTTGTCA AGTATTAGAGTGCCAATTATCGTGCCTGTCATGATGCTGGCTATTAAGGAGGCTTCAGCTGACCTGTCGC CATATGTTAGGAAGAATGCAGCCCATGCTATTCAGAAGCTGTACAGTCTTGACCCCGAGCAGAAGGAGAT GTTAATTGAAGTCATTGAGAAGCTTCTGAAAGACAAGAGCACGCTGGTGGCTGGCAGTGTGGTGATGGCC TTTGAAGAAGTGTGCCCGGATAGAATCGATCTGATTCACAGGAATTACCGAAAGCTTTGTAACCTGCTGG TTGATGTTGAAGAGTGGGGGCAGGTGGTCATAATCCATATGCTAACAAGATACGCTCGGACCCAGTTTGT CAGTCCTTGGAGAGAGGATGGTGGTCTGGAAGACAATGAGAAGAACTTCTATGAATCTGAGGAGGAGGAG GAGGAGAAGGAGAAGAGCAGCAGGAAGAAGTCGTATGCCATGGACCCAGACCACAGACTCTTAATTAGAA ACACCAAGCCGTTGCTTCAGAGCAGGAATGCAGCGGTGGTTATGGCTGTCGCTCAGCTATATTGGCACAT CTCACCCAAGTCGGAGGCTGGTGTCATTTCTAAATCACTGGTGCGCTTATTGCGCAGCAATAGGGAGGTG CAGTATATTGTATTGCAAAATATTGCAACTATGTCAATTGAGAGAAAGGGTATGTTTGAACCTTACCTGA AGAGTTTCTATGTCAGATCCACTGATCCAACTATGATCAAAACACTGAAGCTTGAAATTTTGACAAACTT GGCAAATGAAGCCAACATATCAACTCTTCTTCGAGAATTTCAGACCTACGTGAGAAGCCAGGACAAACAG TTTGCAGCAGCCACTATTCAGACCATAGGCAGATGTGCAACCAGCATTAGCGAGGTCACCGACACATGCC TCAACGGCCTGGTCTGCCTGCTGTCCAACAGGGATGAAATAGTTGTTGCTGAGAGTGTGGTTGTTATAAA GAAATTGTTGCAAATGCAGCCTGCACAGCATGGTGAAATTATCAGACATATGGCCAAGCTCTTGGACAGT ATCACTGTTCCTGTGGCTAGAGCAAGCATCCTTTGGCTAATTGGAGAAAACTGTGAACGAGTTCCTAAAA TTGCTCCTGATGTTTTGAGGAAAATGGCTAAAAGTTTCACTAGTGAAGATGATCTCGTGAAACTGCAGAT TTTAAATCTTGCAGCAAAATTGTATTTAACTAATTCCAAACAGACAAAATTGCTTACCCAGTACATATTA AATCTCGGCAAGTATGATCAAAACTACGACATCAGAGACCGCACACGATTTATTAGGCAGCTTATTGTTC CGAACGAGAAGAGTGGAGCCTTAAGTAAATATGCCAAAAAAATCTTCCTAGCACCGAAGCCTGCGCCACT GCTCGAGTCTCCCTTTAAAGATAGGGATCGCTTCCAGCTTGGCACCTTATCTCATACTCTCAACATTAAA GCGTCCGGGTACCTGGAATTATCTAATTGGCCAGAGGTGGCGCCTGACCCATCGGTTCGCAATGTAGAAG TAATAGAGTCAGCAAAAGAATGGACCCCACTTGGGAAAACAAAGAAAGAAAAACCCATGAAGAAATTTTA CTCTGAATCTGAGGAGGAGGAGGACGAGGACGAGGATGAGGACGAGGAGGAGGAAGAAAAGGAAGATGAG GATGAGAACCCCTCTGATAGCAGCAGTGACAGTGAGAGCGGGTCCGGAAGTGAGAGTGGAGACACAGGCA CCGAGGACAGCAGTGAGGACTCGTCCAGTGGACAAGACAGCGAGACCGGAAGCCAGGCAGAAGCGGAACG CCAGAAGGTGGCCAAGAGGAACTCCAAAACCAAGAGGAAAAGTGATTCAGAAAATAGGGAGAAGAAAAAT GAAAACTCAAAAGCTTCTGAATCGTCCAGTGAGGAGTCCAGTTCCATGGAAGACAGTTCTTCCGAGTCAG AGTCAGAGTCAGGCTCTGACAGTGAGCCTGCGCCCAGAAACGTGGCTCCGGCAAAAGAAAGAAAACCCCA GCAAGAAAGACATCCTCCCTCCAAAGATGTTTTCCTTCTAGATCTGGACGACTTTAACCCAGTATCCACC CCAGTTGCCCTTCCTACACCAGCCCTTTCTCCAAGCTTGATAGCTGATCTTGAAGGTCTAAACTTGTCAA CTTCTTCATCAGTCATCAACGTCAGTACGCCTGTCTTCGTGCCAACAAAAACACATGAGCTGCTTCACCG AATGCATGGGAAAGGACTGGCCGCCCACTACTGCTTCCCAAGACAGCCCTGCATCTTCAGTGACAAGATG GTCTCTGTACAGATCACCCTGACTAATACTTCTGATCGAAAAATAGAAAACATCCACATAGGGGGGAAAG GGCTTCCTGTGGGCATGCAGATGCATGCCTTTCATCCAATAGACTCTCTGGAGCCTAAGGGGTCCGTGAC TGTCTCAGTGGGTATTGACTTTTGTGATTCTACACAGACGGCCAGTTTCCAATTGTGCACCAAGGATGAC TGCTTCAACGTCACCCTCCAGCCACCTGTTGGGGAGCTGCTTTCACCTGTGGCCATGTCAGAGAAGGACT TTAAGAAGGAGCAAGGAACGCTCACTGGAATGAACGAGACTTCGGCTACACTCATCGCTGCTCCACAGAA CTTCACTCCCTCCATGATCCTCCAGAAGGTTGTAAATGTGGCCAACCTCGGTGCTGTGCCTTCCAGCCAA GATAACGTACACAGGTTTGCAGCTAGAACTGTGCACAGTGGGTCGCTGATGCTAGTCACAGTGGAACTGA AGGAAGGCTCCACAGCCCAGCTTATCATCAACACCGAGAAAACCGTGATTGGTTCTGTTCTGCTGCGGGA GCTGAAGCCTGTCCTGTCCCAGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211641 representing NM_009680
Red=Cloning site Green=Tags(s) MSSNSFAYNEQSGGGEAAELGQEATSTISPSGAFGLFSSDWKKNEDLKQMLESNKDSAKLDAMKRIVGMI AKGKNASELFPAVVKNVASKNIEIKKLVYVYLVRYAEEQQDLALLSISTFQRALKDPNQLIRASALRVLS SIRVPIIVPVMMLAIKEASADLSPYVRKNAAHAIQKLYSLDPEQKEMLIEVIEKLLKDKSTLVAGSVVMA FEEVCPDRIDLIHRNYRKLCNLLVDVEEWGQVVIIHMLTRYARTQFVSPWREDGGLEDNEKNFYESEEEE EEKEKSSRKKSYAMDPDHRLLIRNTKPLLQSRNAAVVMAVAQLYWHISPKSEAGVISKSLVRLLRSNREV QYIVLQNIATMSIERKGMFEPYLKSFYVRSTDPTMIKTLKLEILTNLANEANISTLLREFQTYVRSQDKQ FAAATIQTIGRCATSISEVTDTCLNGLVCLLSNRDEIVVAESVVVIKKLLQMQPAQHGEIIRHMAKLLDS ITVPVARASILWLIGENCERVPKIAPDVLRKMAKSFTSEDDLVKLQILNLAAKLYLTNSKQTKLLTQYIL NLGKYDQNYDIRDRTRFIRQLIVPNEKSGALSKYAKKIFLAPKPAPLLESPFKDRDRFQLGTLSHTLNIK ASGYLELSNWPEVAPDPSVRNVEVIESAKEWTPLGKTKKEKPMKKFYSESEEEEDEDEDEDEEEEEKEDE DENPSDSSSDSESGSGSESGDTGTEDSSEDSSSGQDSETGSQAEAERQKVAKRNSKTKRKSDSENREKKN ENSKASESSSEESSSMEDSSSESESESGSDSEPAPRNVAPAKERKPQQERHPPSKDVFLLDLDDFNPVST PVALPTPALSPSLIADLEGLNLSTSSSVINVSTPVFVPTKTHELLHRMHGKGLAAHYCFPRQPCIFSDKM VSVQITLTNTSDRKIENIHIGGKGLPVGMQMHAFHPIDSLEPKGSVTVSVGIDFCDSTQTASFQLCTKDD CFNVTLQPPVGELLSPVAMSEKDFKKEQGTLTGMNETSATLIAAPQNFTPSMILQKVVNVANLGAVPSSQ DNVHRFAARTVHSGSLMLVTVELKEGSTAQLIINTEKTVIGSVLLRELKPVLSQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009680 |
| ORF Size | 3315 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009680.3 |
| RefSeq Size | 3956 bp |
| RefSeq ORF | 3318 bp |
| Locus ID | 11774 |
| UniProt ID | Q9Z1T1 |
| MW | 123.2 kDa |
| Gene Summary | Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203776 | Ap3b1 (untagged) - Mouse adaptor-related protein complex 3, beta 1 subunit (Ap3b1), (10ug) |
CNY 7,720.00 |
|
| MG211641 | Ap3b1 (tGFP-tagged) - Mouse adaptor-related protein complex 3, beta 1 subunit (Ap3b1) |
CNY 10,640.00 |
|
| MR211641L3 | Lenti ORF clone of Ap3b1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, beta 1 subunit (Ap3b1) |
CNY 11,590.00 |
|
| MR211641L4 | Lenti ORF clone of Ap3b1 (mGFP-tagged) - Mouse adaptor-related protein complex 3, beta 1 subunit (Ap3b1) |
CNY 11,590.00 |
