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Trip12 (BC034113) Mouse Tagged ORF Clone
CAT＃: MR211639
- TrueORF®
Trip12 (Myc-DDK-tagged) - Mouse thyroid hormone receptor interactor 12 (cDNA clone MGC:31615 IMAGE:4507222)
ORF Plasmid: tGFP
"BC034113" in other vectors (4)
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CNY 11,020.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC31615 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211639 representing BC034113
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTATCCAGCCAAGACCTGAAGATAGTAGTTGGAGCTCTTCAGATGGCAGAAATCCTAATGCAAAAGT TACCTGATATTTTTAGTGTTTACTTCAGGAGAGAAGGTGTGATGCATCAAGTCAAACATTTAGCAGAATC AGAGTCTTTGTTGACAAGTCCCCCCAAGGCATGTACAAATGGGTCAGGATCCCTGGGATCTACAACACCA GCCAGCAGTGGGACTGCAACGGCAGCAACCAACGCCTCTGCTGATCTGGGGTCACCTAGCTTGCAGCATA GCAGAGATGATTCTTTGGATCTGAGCCCTCAAGGTCGATTAAGTGATGTTCTCAAGAGAAAACGACTGCC AAAACGAGGGCCAAGAAGGCCAAAATATTCCCCTCCAAGAGATGATGACAAAGTAGACAATCAAGCTAAA AGCCCCACCACTACTCAGTCACCTAAATCTTCCTTCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGT TAAGCGCACAGTCCAACAGCAACAATATCGAACCAGCACGAACTGCAGGAGTTAGTGGTCTTGCCAGGGC AGCCTCAAAAGACACCATATCCAATAATAGAGAAAAAATTAAAGGCTGGATTAAGGAGCAGGCACATAAA TTTGTTGAGCGTTATTTTAGTTCTGAGAATATGGATGGAAGCAACCCTGCCTTGAATGTCCTTCAGAGAC TTTGTGCTGCAACTGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAG CATAGTCTCAGAGTCAGATGTTTCTTCATTTGAAATCCAACATAGTGGGTTTGTGAAACAACTGTTGCTG TATTTGACATCTAAGAATGAAAAGGATGCTGTCGGCAGAGAGATCCGGTTAAAACGTTTCCTTCATGTAT TTTTTTCTTCTCCACTTCCTGGAGAAGAGCCTGTTGGAAGAGTAGAACCAGTGGGCCATGCACCTTTGCT GGCATTGGTTCACAAGATGAACAACTGTCTCAGCCAAATGGAGCAATTTCCAGTCAAAGTACATGATTTC CCTAGTGGGAACGGAGCTGGAGGAAGCTTTTCTCTCAACAGAGGATCACAAGCTTTAAAATTTTTCAACA CACATCAGTTAAAATGTCAGTTACAAAGACACCCAGACTGTGCAAATGTGAAGCAGTGGAAAGGCGGGCC TGTCAAGATTGATCCCCTGGCTCTGGTGCAAGCTATTGAGAGATACCTTGTGGTTAGAGGATATGGAAGA GTAAGAGAAGATGATGAAGATAGTGATGATGATGGGTCAGATGAAGAAATAGATGAGTCTTTGGCTGCTC AATTCTTAAATTCGGGAAATGTAAGGCACAGGCTACAGTTTTATATTGGAGAACATTTACTACCATATAA CATGACCGTGTATCAGGCAGTGCGGCAATTCAGTGTACAGGCTGAAGATGAAAGAGAATCTACTGATGAT GAGAGCAATCCTCTGGGAAGAGCTGGAATTTGGACAAAGACTCATACAATATGGTACAAACCTGTGAGGG AGGATGAAGAAAGTACTAAAGATTGTGTTGGTGGCAAAAGGGGGAGAGCCCAAACAGCTCCAACAAAAAC TTCCCCCAGAAATGCAAAGAAGCATGATGAGTTATGGCATGATGGAGTGTGCCCGTCAGTAGCAAATCCT TTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATCACCTTTGAAGACCCATCCTTAGATGTAATAC TTCTTTTAAGAGTTTTACATGCCATCAGTCGATATTGGTATTACTTGTATGATAATGCGATGTGCAAGGA GATTATTCCAACTAGTGAATTTATTAACAGTAAATTAACAGCAAAAGCAAATAGGCAGCTTCAAGACCCT CTAGTAATCATGACAGGAAACATCCCAACATGGCTCACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTC CTTTTGATACCCGACAAATGCTTTTTTATGTAACTGCATTTGATCGGGACCGGGCAATGCAAAGATTACT TGATACCAACCCAGAAATCAACCAGTCTGATTCTCAAGACAGCAGAGTTGCGCCTAGATTGGATAGAAAA AAACGTACTGTGAACAGAGAGGAGCTACTGAAACAAGCTGAGTCTGTGATGCAGGACCTTGGCAGCTCTC GGGCCATGTTAGAAATCCAATATGAAAATGAGGTTGGTACAGGTCTGGGGCCAACACTGGAGTTTTATGC ACTTGTTTCTCAGGAACTACAGAGAGCTGACTTGTGTCTCTGGAGGGGTGAAGAAGTAACTCTTAGCAAT CCAAAAGGAAGCCAAGAAGGGACCAAGTATATTCAAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTA GGACAGCTAAGCCAGCTCACATCGCAAAGGTTAAGATGAAGTTTCGCTTCTTAGGAAAATTAATGGCTAA GGCTATCATGGATTTCAGATTGGTGGACCTTCCTCTTGGCCTGCCCTTTTATAAGTGGATGCTGCGGCAG GAAACTTCATTGACATCTCATGATTTATTTGACATTGATCCTGTTGTAGCCAGATCAGTATATCACCTAG AAGACATTGTCAGACAGAAGAAAAGACTGGAGCAGGATAAATCCCAGACCAAAGAGAGTCTACAATATGC ATTGGAAACTCTTACGATGAATGGCTGTTCAGTGGAAGATCTGGGATTGGATTTTACGCTGCCTGGGTTT CCCAACATTGAATTGAAGAAAGGAGGGAAGGATATTCCAGTTACTATCCACAATTTGGAAGAGTACCTGA GATTGGTGATATTCTGGGCACTAAATGAAGGTGTTTGTCGACAGTTTGACTCATTCAGAGATGGATTTGA ATCGGTCTTCCCGCTCTGCCATCTTCAGTACTTCTACCCAGAAGAGCTGGACCAGCTCCTGTGTGGCAGT AAAGCAGACACTTGGGATGCAAAGACATTGATGGAATGCTGCAGGCCTGATCATGGCTATACTCATGACA GTCGGGCTGTGAAGTTCTTGTTTGAGATTCTCAGTAGTTTTGATAATGAGCAGCAGAGGTTGTTTCTCCA GTTTGTGACTGGTAGCCCACGATTGCCAGTTGGAGGATTTCGGAGTTTAAATCCACCTCTGACAATTGTG CGGAAGACATTTGAATCAACAGAAAACCCAGATGACTTCTTACCATCAGTAATGACTTGTGTGAACTATC TTAAATTGCCGGACTACTCGAGCATTGACATAATGCGGGACAAACTCTTGATAGCAGCAAGAGAAGGCCA ACAGTCGTTCCATCTTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211639 representing BC034113
Red=Cloning site Green=Tags(s) MLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTP ASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAK SPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHK FVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLL YLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDF PSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGR VREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDD ESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANP LEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDP LVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRK KRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSN PKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQ ETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGF PNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGS KADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIV RKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034113 |
| ORF Size | 3309 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034113, AAH34113 |
| RefSeq Size | 3945 bp |
| RefSeq ORF | 3311 bp |
| Locus ID | 14897 |
| MW | 144.7 kDa |
| Gene Summary | E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223054 | Trip12 (untagged) - Mouse thyroid hormone receptor interactor 12 (cDNA clone MGC:31615 IMAGE:4507222), (10ug) |
CNY 12,920.00 |
|
| MG211639 | Trip12 (tGFP-tagged) - Mouse thyroid hormone receptor interactor 12 (cDNA clone MGC:31615 IMAGE:4507222) |
CNY 12,160.00 |
|
| MR211639L3 | Lenti ORF clone of Trip12 (Myc-DDK-tagged) - Mouse thyroid hormone receptor interactor 12 (cDNA clone MGC:31615 IMAGE:4507222) |
CNY 12,920.00 |
|
| MR211639L4 | Lenti ORF clone of Trip12 (mGFP-tagged) - Mouse thyroid hormone receptor interactor 12 (cDNA clone MGC:31615 IMAGE:4507222) |
CNY 12,920.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
