Mov10 (NM_001163441) Mouse Tagged ORF Clone
CAT#: MR211430
- TrueORF®
Mov10 (Myc-DDK-tagged) - Mouse Moloney leukemia virus 10 (Mov10), transcript variant 3
"NM_001163441" in other vectors (2)
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CNY 6,992.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C77703; Mov-10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211430 representing NM_001163441
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTAGCAAGTTCAGCTGCCGAAAGCTCCGGGAGACCGGCCAGAGGTTCGAGAGTTTTCTGGCCGAAC GTGGACTGGACCTGGAGACAGATCGTGAGCGGCTGCGGACGATTTACAACCACGACTTCAAGCCCAGCTA TGGGACCCCTGCCCCTGGCTTCTCCTCCATGCTGTATGGAATGAAGATCGCAAATCTGGCCTTCGTCACC AAGACTCGGGTCAGGTTCTTCAAACTAGACCGCTGGGCTGATGTGCAGTTACCAGAAAAGAGGCGAATAA AGCCAGGGTCGAACATCAGCAAACAACACAGATCACTGTTGGCCAGGATCTTTCACGACAGGGCTGAGTA CCTTCATGGGAAGCATGGGGTAGACGTGGAGGTCCAGGGGCCCCATGAAGCCCGAGACGGGCAACTCCTT ATCCACCTGGATTTGAACCGCAAGGAGGTACTAACCCTACGGCTTCGGAACGGCGGAAGCAAACCTGTCA CCCTCACTCACCTATTCCCACTGTGCTGGACGCCCCAGTTTGTCTTCTACCATGGAGAACAGGACCTGCC CTGCCCACTGGGCCCCGGTGAAAGCTATGAACTCCACATCTACTGTAAGACCAGCATTGTGGGTTACTTC CCAGCCACTGTCCTCTGGGAACTCCTGGGACCCGGGGAGTCGGGAGCAGAAGGAGCAGAAACTTTCTACA TTGCCCGATTCCTGGCGGCTGTCGCCCACAGTCCCCTGGCTGCCCAGTTGAAACCCACAACTCCCTTCAA ACGCCCCCCTCGGCTCACCAGAAACTCTGTGTTGACCAACCGGATCGAGGAAGGAGAGAGGCCCGACCGA GCCAAGGGCTATGAACTAGAGCTAAGTTTGGCCCTGGGGACCTATTACCCACCCATCCTCCTCCGACAAC TGCTCCCTACCCTTCTTCAGGGACCAAGTATCTTCACTGCCCCAAAGGAGGTTGCTGAGATCAAGGCCCA GCTGGAGACAACCCTGAAATCCAGGAACTATGAGGTGAAACTCCGGCTGCTGCTGCACCTGGAAGAGCTG CAGATGGAGCATGACATCCGGCACTATGACCTGGACTCGGTACCCATGACCTGGGACCCTGTGGACCAGA ATCCCAGGCTGCTCACCCTGGAGGTTCCTGGTGTCGCAGAGAGCCGTCCCTCAGTGCTACGAGGTGACCA CCTTTTTGCCCTTTTGTCCTCTGAGACCCAACAGGACGACCCTGTCACCTACAAGGGTTTCGTGCACAAG GTGGAACTGGACCGTGTCAAGCTGAGCTTTTCTACAAGCCTCCTGAGCCGATTTGTGGATGGGCTGACCT TCAAGGTGAACTTTACCTTCAACCGCCAGCCCCTTCGGGTCCAGCACCGGGCCCTAGAGTTGACGGGGCG CTGGGTGCTATGGCCCATGCTTTTTCCTGTGGCCTCCCGTGGGGTCTCGTTGCTGCCCTCAGATGTGAAG TTCAAGCTGTACGATCGGAGTCTGGAGTCAAACCCTGAGCAACTGCAGGCCATGAAGCACATTGTGAGGG GTACCACCCGGCCTGCCCCCTACATCATCTTTGGGCCTCCAGGTACCGGCAAGACTGTCACATTAGTGGA GGCCATCAAACAGGTAGTGAAGCATTTGCCCAAAGCCCACATCCTGGCCTGTGCTCCATCCAACTCAGGG GCTGACCTCCTCTGTCAGCGGCTCCGGGTCCACCTGCCCAGCTCCATCTACCGTCTCCTGGCCCCCAGCA GGGACATCCGAATGGTGCCTGAGGACATTAAGACCTGCTGTAACTGGGATGCTAAGAAGGGAGAATATGT GTATCCTGCTAAGAAGCACCTGCAGCAATATCGGGTCTTAATTACCACCCTCATCACTGCCAGCAGGTTG GTGTCAGCCCAGTTTCCCATCGATCACTTCACACACATCTTCATCGATGAGGCTGGCCACTGCATGGAGC CTGAGAGTCTGGTGGCCATAGCAGGACTGATGGATGTCAAGGAAACGGGCAATCCCGGAGGGCAGCTGGT ACTGGCAGGAGACCCTCGGCAGCTGGGGCCTGTGCTGCGCTCGCCACTAGCCCTGAAGCATGGACTTGGC TACTCTCTGCTAGAGCGCCTGCTCGCCTACAACTCCTTGTATAAGAAGGGCCCCAATGGCTATGACCCCC AGTTCATCACCAAACTGCTCCGCAACTACAGGTCTCACCCCACCATCCTGGACATCCCTAACCAGCTGTA CTACGACGGAGAGCTGCAGGCCTGCGCAGATGTGGTGGATCGAGAACGGTTCTGCCGCTGGGAGGGGCTG CCTCAGCAGGGCTTCCCCATCATTTTCCATGGTGTAATGGGCAAAGATGAGAGAGAAGGCAATAGCCCAT CCTTCTTCAACCCTGAAGAGGCTGCTACAGTGACGTCATACCTGAAACAACTCCTGGCCCCTTCCTCCAA GAAGGGTAAAGCCCGCCTGAGCCCCCGAAATGTGGGCGTCATCTCCCCGTACCGGAAGCAGGTAGAAAAA ATCCGTTACTGCATCACAAAACTTGACCGAGAACTTCGCGGACTGGATGACATCAAAGATTTGAAGGTGG GCTCTGTGGAAGAGTTCCAAGGCCAAGAACGCAGCGTCATCCTCATCTCCACCGTCCGAAGCAGCCAGAG CTTTGTACAGCTGGATCTAGACTTTAACCTCGGTTTCCTTAAGAACCCCAAGAGGTTCAATGTTGCTGTG ACCCGAGCCAAGGCTTTGCTCATCGTAGTGGGCAACCCCCTCCTCCTAGGCCACGACCCAGACTGGAAAA CGTTCCTGGAGTTCTGTAAAGAAAACGGGGGATATACCGGGTGCCCCTTTCCTGCCAAACTGGACCTGCA GCAGGGACAGGACTTGCTCCAAGGTCTGAGCAAACTCAGCCCCTCTACCTCAGGGCCCCGCCGTCACCAG AATCTCCCCCAGGAGCGGGAGGGTGAAGGGGGCCTGCCCTTACAAGTGGAGCCAGAGTGGAGAAATGAGC TC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211430 representing NM_001163441
Red=Cloning site Green=Tags(s) MPSKFSCRKLRETGQRFESFLAERGLDLETDRERLRTIYNHDFKPSYGTPAPGFSSMLYGMKIANLAFVT KTRVRFFKLDRWADVQLPEKRRIKPGSNISKQHRSLLARIFHDRAEYLHGKHGVDVEVQGPHEARDGQLL IHLDLNRKEVLTLRLRNGGSKPVTLTHLFPLCWTPQFVFYHGEQDLPCPLGPGESYELHIYCKTSIVGYF PATVLWELLGPGESGAEGAETFYIARFLAAVAHSPLAAQLKPTTPFKRPPRLTRNSVLTNRIEEGERPDR AKGYELELSLALGTYYPPILLRQLLPTLLQGPSIFTAPKEVAEIKAQLETTLKSRNYEVKLRLLLHLEEL QMEHDIRHYDLDSVPMTWDPVDQNPRLLTLEVPGVAESRPSVLRGDHLFALLSSETQQDDPVTYKGFVHK VELDRVKLSFSTSLLSRFVDGLTFKVNFTFNRQPLRVQHRALELTGRWVLWPMLFPVASRGVSLLPSDVK FKLYDRSLESNPEQLQAMKHIVRGTTRPAPYIIFGPPGTGKTVTLVEAIKQVVKHLPKAHILACAPSNSG ADLLCQRLRVHLPSSIYRLLAPSRDIRMVPEDIKTCCNWDAKKGEYVYPAKKHLQQYRVLITTLITASRL VSAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMDVKETGNPGGQLVLAGDPRQLGPVLRSPLALKHGLG YSLLERLLAYNSLYKKGPNGYDPQFITKLLRNYRSHPTILDIPNQLYYDGELQACADVVDRERFCRWEGL PQQGFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKQLLAPSSKKGKARLSPRNVGVISPYRKQVEK IRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVILISTVRSSQSFVQLDLDFNLGFLKNPKRFNVAV TRAKALLIVVGNPLLLGHDPDWKTFLEFCKENGGYTGCPFPAKLDLQQGQDLLQGLSKLSPSTSGPRRHQ NLPQEREGEGGLPLQVEPEWRNEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163441 |
ORF Size | 3012 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163441.1, NP_001156913.1 |
RefSeq Size | 3515 bp |
RefSeq ORF | 3015 bp |
Locus ID | 17454 |
UniProt ID | P23249 |
MW | 114 kDa |
Gene Summary | Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (By similarity). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7 (By similarity). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (PubMed:28662698). May function as a messenger ribonucleoprotein (mRNP) clearance factor (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR211430L3 | Lenti ORF clone of Mov10 (Myc-DDK-tagged) - Mouse Moloney leukemia virus 10 (Mov10), transcript variant 3 |
CNY 10,260.00 |
|
MR211430L4 | Lenti ORF clone of Mov10 (mGFP-tagged) - Mouse Moloney leukemia virus 10 (Mov10), transcript variant 3 |
CNY 10,260.00 |