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Clstn1 (BC053843) Mouse Tagged ORF Clone
CAT＃: MR211346
- TrueORF®
Clstn1 (Myc-DDK-tagged) - Mouse calsyntenin 1 (cDNA clone MGC:54504 IMAGE:6315355)
ORF Plasmid: tGFP
"BC053843" in other vectors (4)
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CNY 9,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cstn1, 1810034E21Rik, Cst-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211346 representing BC053843
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCCGCCCTGCGCCCGCGCTGGCCCCGGCCGTCCGGCTGCTGCTGGCGGGGCTGCTGTGCGGCG GCGGGGTCTGGGCCGCGCGAGTTAACAAGCACAAGCCATGGCTCGAGCCCACCTACCATGGGATAGTCAC CGAGAACGATAACACGGTGCTGCTCGACCCTCCCCTCATCGCCCTGGATAAAGATTCACCGCTCCGCTTT GCAGGTGAGATTTGTGGCTTTAAAATTCACGGGCAGAATGTCCCCTTTGATGCCGTGGTAGTGGATAAGT CCACGGGTGAGGGAATAATCCGGTCAAAAGAGAAACTGGACTGTGAACTACAGAAAGACTACACATTCAC CATCCAGGCCTACGACTGCGGGAAGGGCCCCGATGGCACCGGTGTGAAAAAGTCTCACAAAGCGACCGTG CACATCCAGGTGAACGACGTGAACGAGTACGCCCCCGTGTTCAAGGAGAAGTCGTACAAGGCGGCCGTGG TGGAGGGCAAGCAGCACAGCAGCATCCTCAGGGTGGAGGCTGTGGACGCAGACTGCTCCCCGCAGTTCAG CCAGATCTGCAGCTACGAGATTCTCACCCCAGACGTGCCGTTCACTGTGGACAAAGACGGTTATATTAAG AACACAGAGAAGCTAAACTATGGCAAAGAGCATCAGTATAAGCTCACAGTCACAGCCTACGACTGTGGGA AGAAAAGAGCCACCGAGGACGTCCTAGTGAAGATAAGCGTCAAGCCCACCTGCAGCCCCGGGTGGCAAGG CTGGAGCAGCAGAATTGAGTATGAGCCCGGCACGGGTGCTTTGGCTGTCTTCCCGAGCATCCACTTGGAG ACCTGTGACGAGCCTGTCGCCTCTGTGCAGGCCACGGTGGAGCTGGAGACCAGCCACATAGGGAAAGGCT GCGACCGAGATACCTACTCTGAGAAGTCCCTTCACCGGCTCTGTGGGGCTGCTGCCGGCACCTCAGAGCT GCTCCCGTCCCCCAGTAGCTCCTTCAACTGGACTGTGGGCCTCCCCACCGACAACGGCCATGACAGTGAC CAGGTCTTTGAGTTCAACGGCACGCAGGCCGTGAGGATTCCAGACGGCGTTGTGACCCTTGACCCTAAAG AACCCTTTACAATCTCCGTGTGGATGCGGCATGGGCCGTTTGGCCGGAAGAAGGAGACAATTCTCTGCAG TTCGGATAAGACAGATATGAACCGACACCATTATTCGCTCTACGTCCACGGGTGCAGGCTAGTTTTCCTC CTCCGTCAGGACCCGTCTGAGGAGAAGAAATACAGGCCTGCGGAGTTCCACTGGAAGCTGAACCAAGTCT GTGATGAGGACTGGCACCACTTTGTCCTCAATGTGGAAGTTCCAAGTGTGACTCTCTACGTGGACGGCAT CCCTCATGAGCCCTTCTCTGTGACAGAGGACTACCCGCTTCATCCAACCAAAATAGAGACTCAGCTTGTG GTCGGAGCTTGCTGGCAAGAGTATTCAGGAGTGGAGAGTGGCAATGAGACTGAGCCTGCGACGATGGCCT CTGCAGGTGGCGACCTGCACATGACACAGTTTTTCCGAGGTAACCTGGCTGGCCTCACAGTCCGTTCTGG GAAACTGGCGGATAAGAAGGTGATTGACTGTCTCTACACCTGCAAGGAGGGGCTGGACCTGCAGGTCCCT GAAGATGCCAACAGAGGCGTGCAGATCCAAGCAAGCTCCAGCCAGGCGGTCTTGACCTTGGAGGGAGACA ACGTTGGAGAGCTGGATAAGGCTATGCAGCACATTTCCTACCTTAACTCAAGGCAGTTCCCCACACCAGG CATCCGCAGGCTCAAAATCACCAGCACCGTCAAGTGTTTCAACGAGGCGGCTTGCATCGAGGTGCCCCCG GTGGAAGGCTACGTGATGGTTTTGCAGCCGGAGGAGCCTAAGATCAGCCTGAGTGGCGTCCACCACTTTG CCCGCGCAGCTTCTGAGTTTGAGAGCGCGGAGGGTATTTCCCTCTTCCCTGAGCTGAGAATCATCAGCAC CATCACCAGAGAGGTGGAGCCCGAGGCAGATGGGTCCGAGGACCCCACAGTGCAAGAATCCCTGGTGTCA GAGGAAATTGTGCATGACCTGGACACCTGTGAGGTCACAGTGGAAGGGGATGAGCTGAACGCAGAGCAGG AGAGCCTGGAGGTAGACGTGACCCGCCTCCAGCAGAAGGGCATCGAAGCAAGCCACTCTGACCTGGGTGT GGTCTTCACAGGCGTGGAGACCATGGCAAGCTACGAGGAGGTTTTGCACCTCCTTCGCTATCGGAATTGG CACACCAGGTCCCTGCTGGATCGCAAGTTCAAGCTCATTTGCTCAGAACTAAATGGTCGCTACCTCAGCA ATGAGTTCAAGGTGGAGGTGAATGTGATCCACACAGCCAACCCCGTGGAACATGCCAACCACATGGCTGC CCAGCCACAGTTCGTCCACCCTGAACATCGATCCTTTGTGGACCTGTCTGGTCACAACCTGGCCAATCCT CACCCGTTTGCAGTTGTCCCCAGCACGGCGACAGTGGTGATTGTGGTGTGTGTCAGCTTCCTGGTTTTCA TGATCATCCTGGGGGTGTTTCGGATCCGGGCTGCACATCAGCGAACGATGCGGGACCAGGACACGGGGAA GGAGAATGAGATGGACTGGGATGACTCTGCCTTGACCATCACCGTCAACCCCATGGAGACATATGAGGAC CAGCACAGCAGTGAGGAGGAGGAAGAGGAGGAGGAGGAGGAGGAGAGCGAAGATGGGGAGGAAGAGGAAG ACATCACCAGTGCCGAGTCTGAGAGCAGTGAGGAGGAGGAAGGAGGCCCCGGAGACGGCCAGAATGCCAC CCGACAGCTGGAATGGGATGACTCCACACTCAGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211346 representing BC053843
Red=Cloning site Green=Tags(s) MLRRPAPALAPAVRLLLAGLLCGGGVWAARVNKHKPWLEPTYHGIVTENDNTVLLDPPLIALDKDSPLRF AGEICGFKIHGQNVPFDAVVVDKSTGEGIIRSKEKLDCELQKDYTFTIQAYDCGKGPDGTGVKKSHKATV HIQVNDVNEYAPVFKEKSYKAAVVEGKQHSSILRVEAVDADCSPQFSQICSYEILTPDVPFTVDKDGYIK NTEKLNYGKEHQYKLTVTAYDCGKKRATEDVLVKISVKPTCSPGWQGWSSRIEYEPGTGALAVFPSIHLE TCDEPVASVQATVELETSHIGKGCDRDTYSEKSLHRLCGAAAGTSELLPSPSSSFNWTVGLPTDNGHDSD QVFEFNGTQAVRIPDGVVTLDPKEPFTISVWMRHGPFGRKKETILCSSDKTDMNRHHYSLYVHGCRLVFL LRQDPSEEKKYRPAEFHWKLNQVCDEDWHHFVLNVEVPSVTLYVDGIPHEPFSVTEDYPLHPTKIETQLV VGACWQEYSGVESGNETEPATMASAGGDLHMTQFFRGNLAGLTVRSGKLADKKVIDCLYTCKEGLDLQVP EDANRGVQIQASSSQAVLTLEGDNVGELDKAMQHISYLNSRQFPTPGIRRLKITSTVKCFNEAACIEVPP VEGYVMVLQPEEPKISLSGVHHFARAASEFESAEGISLFPELRIISTITREVEPEADGSEDPTVQESLVS EEIVHDLDTCEVTVEGDELNAEQESLEVDVTRLQQKGIEASHSDLGVVFTGVETMASYEEVLHLLRYRNW HTRSLLDRKFKLICSELNGRYLSNEFKVEVNVIHTANPVEHANHMAAQPQFVHPEHRSFVDLSGHNLANP HPFAVVPSTATVVIVVCVSFLVFMIILGVFRIRAAHQRTMRDQDTGKENEMDWDDSALTITVNPMETYED QHSSEEEEEEEEEEESEDGEEEEDITSAESESSEEEEGGPGDGQNATRQLEWDDSTLSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053843 |
| ORF Size | 2907 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053843.1 |
| RefSeq Size | 4486 bp |
| RefSeq ORF | 2909 bp |
| Locus ID | 65945 |
| MW | 164.5 kDa |
| Gene Summary | Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220810 | Clstn1 (untagged) - Mouse calsyntenin 1 (cDNA clone MGC:54504 IMAGE:6315355), (10ug) |
CNY 9,120.00 |
|
| MG211346 | Clstn1 (tGFP-tagged) - Mouse calsyntenin 1 (cDNA clone MGC:54504 IMAGE:6315355) |
CNY 10,640.00 |
|
| MR211346L3 | Lenti ORF clone of Clstn1 (Myc-DDK-tagged) - Mouse calsyntenin 1 (cDNA clone MGC:54504 IMAGE:6315355) |
CNY 11,590.00 |
|
| MR211346L4 | Lenti ORF clone of Clstn1 (mGFP-tagged) - Mouse calsyntenin 1 (cDNA clone MGC:54504 IMAGE:6315355) |
CNY 11,590.00 |
