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1500010J02Rik (BC060629) Mouse Tagged ORF Clone
CAT＃: MR211335
- TrueORF®
1500010J02Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1500010J02 gene (cDNA clone MGC:79217 IMAGE:6847619)
ORF Plasmid: tGFP
"BC060629" in other vectors (3)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RP23-19I2.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211335 representing BC060629
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTTGGTGAATTAGCCCAAGCTGGCAGCCAGGTGTCCATCATTGTACAGATCCCTGCTCAGATGG TGTGGCACCGAGTCCTCCGGCCTGGCAGGGCCTATGTACTGACAAAATTGCAAGTAACCAAGACCCGGAT TCACCTTTCCTGTATTTGGACAACCATTCCATCTTCAACTCTGAAGCCTCTGAGACCAGGTTATGTGCAA GAGCTGGAGCTGGACTTAGAGTTCTCGAAAGCTGACCTTAAACCACCACCCCAGCCCACCAGCTCCAAAG ACAGCAGAGGGCAAGAAGGTCTTGTCCGGGCTTCCAAAGTCTTGCATTATTTGGGAACAGTCACTGCTGT GTTGCATGAGTCTGCCGGCCTCTACATTCTAGATGGGCAGCTGATTCTCTGCCTCGCATACCAGAAGATC CATGGCCTCAGGCGGGTTATTCGACCAGGGGTCTGTCTGGAGCTCCGAGATGTTCATCTCCTCCAGGCAG TGGGTGGAGCAACAACAAAACCTGTCCTGGCGCTCTGTCTCCATGGCACTGTTCGGCTTCAAGGCTTTTC TTGTCTTAAGCCTCTGACTTTGCCGTCCTCCAAAGTTTATGGTGCCTCCTTGTATGAGCAACTCGTGTGG AAATGTCAGTTAGGACTTCCCTTATACTTATGGGCTGCCAAGACCCTGGAGGATCTCATCTACAAGCTGT GTCCCCATGTGTTGAGATGTCACCAGTTCCTGAAGCAACCCTCTCCTGGGAAGCCCAGCTTGGGCCTGCA GCTCCTGGCTCCCTCCTGGGATGTTCTGATTCCACCTGGCAGCCCCATGCGGCATGCGTACAGCGAGATC CTGGAAGAACCACACAACTGCCCCCTTCAGAAATACACTCCACTGCAGACTCCCTACTCTTTCCCTACTA TGCTCGCCCTGGCAGAGGAAGGGCAGCACAGGGCTTGGGCTACTTTTGACCCAAAGGCCATGCTGCCTCT CCCAGAAGCCAGTCACCTGACCAGCTGCCAACTGAATCGCCACCTAGCCTGGTCCTGGGTCTGTCTGCCA TCCTGTGTGTTCCAGCCAGCCCAGGTTTTACTTGGGGTCCTGGTGGCTTCATCTCGTAAAGGTTGTCTGG AGCTTCGGGATCTAAGAGGTTCACTGCCTTGTATCCCCTTGACTGAGAGTTCACAGCCCCTCATTGACCC GAATCTCGTAGGCTGCCTGGTGCGGGTAGAGAAGTTCCAGTTGGTAGTAGAGAGAGAAGTCAGGAGCAGC TTCCCTTCCTGGGAGGAGATGGGCATGGCACGCTTCATCCAGAAGAAGCAGGCCAGAGTCTACGTCCAGT TCTACCTGGCTGATGCCCTGATCCTGCCCGTGCCCAGACCCACCTTTGGTTCAGAACCATCTCAGACAGC CTCCTCCTGCCCAGAGGGACCCCACCTGGGACAGAGCCGGCTGTTCTTACTGTCCCACAAGGAGGCCCTC ATGAAACGTAATTTTTGTCTCCTTCCGGGAGACAGTTCACAACCTGCAAAGCCCACCTTGAGTTTCCACG TGTCAGGAACTTGGCTTTGTGGCACACAGAGGAAGGAAGGTTCTGGATGGAGCCCACCCGAGTCTCTGGC CGTTGAGAGCAAGGATCAGAAGGTCTTCCTCATCTTTCTTGGCTCTTCAGTTCGATGGTTTCCGTTCTTG TACCCAAACCAAGTCTACCGACTCGTGGCTTCCGGCCCCTCACAGACACCAGTGTTCGAGACAGAGGGCT CAGCTGGCACCTCTCGACGTCCTCTGGAACTGGCTGACTGTGGATCTTGCCTCACTGTCCAAGAGGAGTG GACCCTGGAGCTTGGGAGCTCTCAGGACATACCTAATGTACTGGAGGTCCCTAGAACACTGCCCGAGTCC TCACTGGCCCAGCTGCTCGGTGACAACTCCCCCGATTCCTTGGTATCTTTCTCTGCTGAGATTTTGTCTC GGATACTGTGTGAACCACCTCTGGCCCTTCGCCGGATGAAACCTGGGAATGCTGGGGCTATTAAAACAGG TGTGAAGCTAACAGTAGCTCTAGAAATGGATGACTGTGAATATCCCCCTCACCTGGACATATATATCGAA GATCCACAGTTGCCTCCCCAAATAGGACTCCTTCCAGGAGCCCGAGTCCACTTTAGCCAGCTGGAGAAAA GGATTTCCAGATCCAACATTGTTTACTGTTGTTTCCGGTCATCCACTTCTGTGCAGGTCCTGAGCTTCCC CCCAGAGACCAAAGCCAGTGCTCCTCTGCCCCACATCTATCTGGCTGAACTTCTCCAAGGTGACCGGCCT CCCTTCCAGGCCACTACCTCTTGCCATATTGTGTATGTCCTGAGCCTTCAGATCCTGTGGGTGTGTGCTC ATTGCACCAGCATCTGCCCCCAGGGTAAGTGTAGTCGCCGGGACCCCAGTTGTCCATCTCAGAGGGCTGT AAGCCAGGCCAACATCAGGCTCCTGGTAGAAGATGGCACCGCAGAAGCGACGGTGATCTGTAGGAATCAT CTTGTGGCAAGGGCACTAGGGCTGAGTCCTAGTGAGTGGTCCTCCATCCTCGAGCATGCCAGGGGGCCGG GGAGAGTGGCCTTGCAGTTTACAGGGCTAGGAGGCCAAACAGAGTCTGCAAGCAAAACCCATGAGCCCTT GACCCTGCTCCTCCGGACACTTTGTACCAGCCCCTTTGTCCTCCGACCCGTTAAGCTTTCTTTTGCACTT GAAAGGAGACCCACTGATATTTCCCCACGAGAACCGTCCCGGCTGCAGCAGTTCCAGTGTGGAGAGCTCC CTCTGCTGACCAGAGTGAATCCCAGGCTCCGACTGGTCTGCCTTTCTCTGCAGGAGCCGGAGCTCCCCAA CCCTCCACAAGCATCTGCTGCTTCGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211335 representing BC060629
Red=Cloning site Green=Tags(s) MTLGELAQAGSQVSIIVQIPAQMVWHRVLRPGRAYVLTKLQVTKTRIHLSCIWTTIPSSTLKPLRPGYVQ ELELDLEFSKADLKPPPQPTSSKDSRGQEGLVRASKVLHYLGTVTAVLHESAGLYILDGQLILCLAYQKI HGLRRVIRPGVCLELRDVHLLQAVGGATTKPVLALCLHGTVRLQGFSCLKPLTLPSSKVYGASLYEQLVW KCQLGLPLYLWAAKTLEDLIYKLCPHVLRCHQFLKQPSPGKPSLGLQLLAPSWDVLIPPGSPMRHAYSEI LEEPHNCPLQKYTPLQTPYSFPTMLALAEEGQHRAWATFDPKAMLPLPEASHLTSCQLNRHLAWSWVCLP SCVFQPAQVLLGVLVASSRKGCLELRDLRGSLPCIPLTESSQPLIDPNLVGCLVRVEKFQLVVEREVRSS FPSWEEMGMARFIQKKQARVYVQFYLADALILPVPRPTFGSEPSQTASSCPEGPHLGQSRLFLLSHKEAL MKRNFCLLPGDSSQPAKPTLSFHVSGTWLCGTQRKEGSGWSPPESLAVESKDQKVFLIFLGSSVRWFPFL YPNQVYRLVASGPSQTPVFETEGSAGTSRRPLELADCGSCLTVQEEWTLELGSSQDIPNVLEVPRTLPES SLAQLLGDNSPDSLVSFSAEILSRILCEPPLALRRMKPGNAGAIKTGVKLTVALEMDDCEYPPHLDIYIE DPQLPPQIGLLPGARVHFSQLEKRISRSNIVYCCFRSSTSVQVLSFPPETKASAPLPHIYLAELLQGDRP PFQATTSCHIVYVLSLQILWVCAHCTSICPQGKCSRRDPSCPSQRAVSQANIRLLVEDGTAEATVICRNH LVARALGLSPSEWSSILEHARGPGRVALQFTGLGGQTESASKTHEPLTLLLRTLCTSPFVLRPVKLSFAL ERRPTDISPREPSRLQQFQCGELPLLTRVNPRLRLVCLSLQEPELPNPPQASAASS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060629 |
| ORF Size | 2898 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060629.1 |
| RefSeq Size | 3247 bp |
| RefSeq ORF | 2900 bp |
| Locus ID | 68964 |
| MW | 106.6 kDa |
| Gene Summary | Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha. The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Involved in telomere maintenance. Involved in genome stability (By similarity). May be in involved in telomeric C-strand fill-in during late S/G2 phase (PubMed:22748632).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211335 | 1500010J02Rik (tGFP-tagged) - Mouse RIKEN cDNA 1500010J02 gene (cDNA clone MGC:79217 IMAGE:6847619) |
CNY 10,550.00 |
|
| MR211335L3 | Lenti ORF clone of 1500010J02Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1500010J02 gene (cDNA clone MGC:79217 IMAGE:6847619) |
CNY 11,500.00 |
|
| MR211335L4 | Lenti ORF clone of 1500010J02Rik (mGFP-tagged) - Mouse RIKEN cDNA 1500010J02 gene (cDNA clone MGC:79217 IMAGE:6847619) |
CNY 11,500.00 |
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