4933427D14Rik (NM_028963) Mouse Tagged ORF Clone
CAT#: MR211309
- TrueORF®
4933427D14Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4933427D14 gene (4933427D14Rik)
ORF Plasmid: tGFP
"NM_028963" in other vectors (4)
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CNY 7,024.00
CNY 7,130.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C85113; Kiaa0753; MNR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211309 representing NM_028963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCAAGCCAGCCAGCTTCTACATGTGTTCATCTAGCACCCAGTCTCCAACTGGATGCCATGAGTG ACCCCAGGAACCTTCAGCCCCAGAACCAGCTGCAATTTAATAGGAATGTTCCTACAAATCCCAGCAACTT GGCAGTCCGGTACTCTTGCCCACATGGAATTAAAATTGAAAAACTGAAACACTCATACAATGAATTGAAT CATGGTAAAGACTTGGATTTTAAAGCTGGAAATGAATTAAGCAGTTCTGTCTCATTTTCTGTCATATCAG AAGAGAGGCTTAGCTATGCTGTCCACCTAGCCAAGAGAGATGTAAAACGAAGACAATTTGAAGAACACGT GAAAGATCATCTCAGAAGTCTGCCCCATAGCTCTCACAAAGGTATGCACACCAAGGTGGAAAGGAAGGAC TCCAAGAGTCAGGATGTCTGTCATTGCAGCCACCAGCCGTCCAGAGTAAGCCTCTCCAGCTCGGGCGCTA AGGTGTATGTGTACACATCTCAGCCAGGACGAGCTGATCTTACTGTGCCAAATTCACCACCTACCCATGA TCCAGGACTTCAGCCGCAACCCAGAATAGAGGAGAACAAAAACCTGTGGGAACAGAAAGGCCTGCTAGAA GTTCAGCGACTCCAAAAGGAATTGAGTGGTTGTATCCGTAAGATAGAAGCACTAACGAAAAAAGATAGAC TAGAAGAGGCTTTGGATCCAGATGAAGAGCATCGAATCCGAGTCAGGAGACAGGAGCAAGCCGTGCGCTG TGCCCGCATGCTCTACGTTCTTCAGCAGCAGGTAAAAGAAATCCAGGAAGAATTGGATAAATTGAGTCCA CAAAAAATTAAACATACTAAGAAGTCATGGGCAGTGTCTAGGCTGGCAGCCGCTCATCGAGCAGCCATCC GGGCCTTGCAAGTGTTTGTCACTCAGTTTACTGACCGAGGGGAGCACCCAGTTCCTGCTCGGTGTAGGGA GCTGGGCAGCCTTATTCGTCAGCTGTCGCTGTGTTCCGCCAAGCTGGATGCTGATCCTTCTGTGCCAGAC GTGGTCATAGACATCTTGCAGCAAATCGAGGCTTTGGAGTCTCTCCTGGAAAAGAAATTGTCACCTAAAA AGGCAAAGAAATGTTTCACAGAAATTCGGAGCAGATTTCCTGTTGGTAGCCAAAAGATCTTAGAGAGATG GCCAACTGTACTGCCCAAAAGTGAGAGGAGACCCTTGGTCACAAAGGAGACATTCCCACAGGAAACCAGT CGACCTCCTGTGGCAAAGAGGCTTCTTGCAGCTATGTGTCAGCCTGATGGGGAGCTTCAGAGGCTGGAGA GTGAACCCGATGTGCTGGATGCACATCTTCTTCCAGAAGAAGCCCCTCGTATAGAGGACAATGGTACAGA CTTCAAAGACGGAACATTAACCCCAGCCAAACCACAGGCAGTGAGAAGGAAGGCTTTGACAGGGTCCATG CCGATTAGGAAGAAAGATACTGTGGAGTCAGCCAGACCACAGCAGGGACTCCACATAGCTGAAAGAAATC GACCAAACCAACCTTACAGCAAAAGCAGGTTACAGCAGACAACAGTGTCATCCAGATTAAAAATGAACCG ACAGCCTATGAAAGACCATAGGGCTCCTTGGATACCTCCAAACCCCACATCCCCACCAGCCTCTCCTAAA TGTGCTGCGTGGATGAAGGTGAAGTACAGCCCCAGGGATGCTGCAAAGGAGCAGTCCCTACAGCAAGAGG ATACCCACAAGGAAAGTCAGCTGAGAGGTGATGCTGAGCAAGAAGCAGCCAGGCTTTCTTGGCCAGATGC TGAGTCTTCCAAAAGACTGAAGGAACTAGAAGAATTGGAAGCCAAAGAAATGGAAAGAAAGCAAAAGCAG AGGCTCAATTGGCTTGAGGCTGAGACTTCTAGAAGAACCAAAGAGTTGGATGAACTGAAAGCTGAAGAGA TGGATAGACTTCAAAAATTAAGTGTCTCTGCCACCCAGTTAGCTGACAAGGTGGAGGAGGCAGTTCTGGA GCGGTTGAAGCCCCTCTTGATCAAGGCCCAGAGAGTGAATTCTTCGGTGGAAGCAAATAGTCATTTGAAG GATCGTCCGTCACGGCACGCAGCAGCAGCAGCCCAGCCTGCCGAGCAGGCTTCAGATGTACGTTTTGAAT CCAGGAATATTCCTCAGCTGCGTGACTGTTTGGAAGATACTGCTCATGAGCTCTGGGCTAGGACTCAGGA TAAGATCTTGGGGTCAGAAACTTCAGCCAGATTGGGGGACAGCAAGGACAGCCCAGATCTAGAGACCATG ATGCTCCGGATGGAAGAAATGGAAAAATACCAGGAGACAGTTCGCCAAAGATATAATAAAATTGTATACG CTGACCCTCACTTATGGATGCACGAAGAAAGAAATGACCAGAACACCCCAGCTGTAAGTGAAGGACCTTT GGCTTCCCACCCAATCAAGATCACCAAGACAGCAACTCAAAAGTGCCCTGCTGTGAACATCTTGTTAGAA AGGCCCTGCAATGCCAACTCACTGGATGAAAGTGTAACAACAGAGGAGGGATCGGAGAAAAGGGAGGCTC CCCTGCCCCTCTCCAGAGAAGACCTGCATCAGAGAAAAGGGCAAACTCCCCTCTCAGTCCCACCCAGGAT GCGGCACAGCATTGGTGCTTACTGTAGCCGGTTTGAGCAATTCCTACGGATCATAGCTCATGAGGCTATA GGCTCCTTCAACCCATGGCTAATAGCTGAAAGCTTTTCAGATGAGCTGGTAGATGAAGCACTGGGGGCCG TGGCTGCAGAACTGCAGGATGTGTGTGAAGACTATGCAGAAGCTGTGTTCACCTCAGAGTTCCTAGAGGC CGCTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211309 representing NM_028963
Red=Cloning site Green=Tags(s) MGPSQPASTCVHLAPSLQLDAMSDPRNLQPQNQLQFNRNVPTNPSNLAVRYSCPHGIKIEKLKHSYNELN HGKDLDFKAGNELSSSVSFSVISEERLSYAVHLAKRDVKRRQFEEHVKDHLRSLPHSSHKGMHTKVERKD SKSQDVCHCSHQPSRVSLSSSGAKVYVYTSQPGRADLTVPNSPPTHDPGLQPQPRIEENKNLWEQKGLLE VQRLQKELSGCIRKIEALTKKDRLEEALDPDEEHRIRVRRQEQAVRCARMLYVLQQQVKEIQEELDKLSP QKIKHTKKSWAVSRLAAAHRAAIRALQVFVTQFTDRGEHPVPARCRELGSLIRQLSLCSAKLDADPSVPD VVIDILQQIEALESLLEKKLSPKKAKKCFTEIRSRFPVGSQKILERWPTVLPKSERRPLVTKETFPQETS RPPVAKRLLAAMCQPDGELQRLESEPDVLDAHLLPEEAPRIEDNGTDFKDGTLTPAKPQAVRRKALTGSM PIRKKDTVESARPQQGLHIAERNRPNQPYSKSRLQQTTVSSRLKMNRQPMKDHRAPWIPPNPTSPPASPK CAAWMKVKYSPRDAAKEQSLQQEDTHKESQLRGDAEQEAARLSWPDAESSKRLKELEELEAKEMERKQKQ RLNWLEAETSRRTKELDELKAEEMDRLQKLSVSATQLADKVEEAVLERLKPLLIKAQRVNSSVEANSHLK DRPSRHAAAAAQPAEQASDVRFESRNIPQLRDCLEDTAHELWARTQDKILGSETSARLGDSKDSPDLETM MLRMEEMEKYQETVRQRYNKIVYADPHLWMHEERNDQNTPAVSEGPLASHPIKITKTATQKCPAVNILLE RPCNANSLDESVTTEEGSEKREAPLPLSREDLHQRKGQTPLSVPPRMRHSIGAYCSRFEQFLRIIAHEAI GSFNPWLIAESFSDELVDEALGAVAAELQDVCEDYAEAVFTSEFLEAAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028963 |
ORF Size | 2877 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028963.1 |
RefSeq Size | 4332 bp |
RefSeq ORF | 2880 bp |
Locus ID | 74477 |
UniProt ID | Q6A000 |
MW | 109.2 kDa |
Gene Summary | Involved in centriole duplication. Positively regulates CEP63 centrosomal localization. Required for WDR62 centrosomal localization and promotes the centrosomal localization of CDK2.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
A ciliopathy complex builds distal appendages to initiate ciliogenesis
,null,
The Journal of Cell Biology
,PubMed ID 34241634
[4933427D14Rik]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222839 | 4933427D14Rik (untagged) - Mouse RIKEN cDNA 4933427D14 gene (4933427D14Rik), (10ug) |
CNY 12,260.00 |
|
MG211309 | 4933427D14Rik (tGFP-tagged) - Mouse RIKEN cDNA 4933427D14 gene (4933427D14Rik) |
CNY 7,890.00 |
|
MR211309L3 | Lenti ORF clone of 4933427D14Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4933427D14 gene (4933427D14Rik) |
CNY 9,030.00 |
|
MR211309L4 | Lenti ORF clone of 4933427D14Rik (mGFP-tagged) - Mouse RIKEN cDNA 4933427D14 gene (4933427D14Rik) |
CNY 9,030.00 |
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