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Gprasp1 (BC058970) Mouse Tagged ORF Clone
CAT＃: MR211303
- TrueORF®
Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:67190 IMAGE:6826050)
ORF Plasmid: tGFP
"BC058970" in other vectors (4)
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CNY 7,008.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GASP1, GASP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAGGGCAGAGGTTGAGCCTGGTGCCCAGGCAAAGGCTGAAAATAAGCCTGGGGATGAGAATGCTA ATGCTGCTGAAGTAGAGCCTGAAGCCCCATTGGTGGTCAGACCCAAGGTTAGGACACAAATCATGACTGG AGCAAGGCCCAAAGTGAAGCCTAAGGGTACCCCTGGAGCAAGACCTAAAGGTGAAACCAGTACACCAGGT GGGGCATATGCCAAATGTAAACCTAAGGCAATCCCTATTGCTCGATCCAAGCATGATGCCCAAGTGTGGG CCCCAAATAAGTTTCGTGGCGAGTCCATGTCAAAGATGGGAAAGCAATGTCAGATCAGTGCTGCAGACCC TCCACTGCTCAGTAATGATTCTGGGATGGTTGCTCAAGCTAAGTGCCTGCCTGTAGATAGAGAACTTGCT AATATGGACACTGAGAGTATTCCCAAAAAGGCTAATTCTCCAGCTGGATTCCAGCCTTCCTATGGCTCAG AGGAGGGAACCAATATGGGGTCCTGGTACCGTGCCAGGCCTGTCCCCAAAGGAGAGGCCTATGAGAATTC CGATTTCAAATGGGCAGATAAACCCTCAGGAAGTCCCTCCTTTTGGAATAGAGATGAAGCCAGTACAAGA TTTCGTCCTAGGAAAAGCATGAAGGCCAATAACAGGTTCAGGCATATGGCCAAACAAGAGGCAAATACCA TGCCTAGGCACAAAAACAAACAAGAGTTCTATAATATTTCCAGTTCTGATTCTGAGGATGAGTCTGGTAA AACTCCCTGGTTCTGGCCCAAAGACAAGACCAAAGTCTGGTCTAAGCCCAAAGAAGAGCCCAATAGTAGG TCCTGGTTTAGGTCTAAGAAAGAAGTCCGTGTTGAATCCACTTCTGGGTCTGAATGTGAAAATCCTACAA AATCTTTGTTTTGGTCTGGAGAAGAGGCTAAATCTAGATCCAAACCCAGAGCCAGGAAAGGGGTCAATAT GAGGGCCAGGCAGCAGGCCAAGCGAGAAGCTTGCAGTGATGCCATGTCTGGAGCTATAGATACAAATAAG AAAGAGTCCTGGTTCTTGCCTGAAGAGAAAGCTAATGTTTTTTCAAAGTCTAAGACAAAGAAAGAGCCCA GAACCAGAGCAGTGCCAAAGGAAGAAGTCAAAACTAAGGCCAGAGCAAGTACCAAGCAAGAAGCCAGGCC AGAGGAGGAAGTCCTCGTTGGGGCTTGGTTCTGGGACACCCAGGAGTCCACTATGGCAGATAGGATAAGC ATCAAGACCACTTTTGTAGAGGAGGAGCCCATTGTTGGAGATTGGTTCTGGAGTGAAGAAGAAGCCAGTG TGGACTCTGAGACCTGTCATACATCCAGACCCAGGGCTAAGGAGGAGCAAGTTAGTAGTTTCTGTTTAGG GTCTGGAAAGAAGAGCAGTATGGAAAGTGGGCCAAAGGCTACTTCTAAATCTATGCCAGTAGCTAAGGAG GATGAGGTCGTTATTGGGTCCTGGTTCTGGGCAGATGATGAAGAGATCAACTTACAGGCTGATGACGAGT CTATTTTTGGATCTTGGTTCTGGGGCACTGGTGAGAACAGCCTTAGATCTGTTGGAGTCAATTGTGAGAA GATGCCGAAGGCTGGAGAAAAAGAAGTTACTGATTCCTGGTTCTGGGCTGGAGATGTCAATACAGAGGCT GAGGTTGAAGAACAGGCCAGGTCGGCATCTACAAAAGCAACAATCTTTGTGCCTTGGTTTTGGTCTGAAA AGCAGCCGAACATGGATTTAGGGTCTGAACCTTGCTCAGATATCATGGCAGGAGCTGAGGAGGAGCCCAT AATTGGGCCCTGGTTCTGGGCTAAAGTAGATAATAGTGTGGAGGCTGAAGTTAACAGTAAGTCTAGCCTT GAGGATGAGGAAGAACCCATTAGATCCCCTTGGTTTGGGGCCAGAGAACAAACCGATATGAAGTATGCAG CTGGTATCAGATACAAGCCTATGGCAGAAGCTGAGGATGCTAACAAAAAGTCTTGTGTCTGGGCAAAAGA ACCCTGTTTGTATCCTACCAATAGAGAATGCTTGAAATCTACTCTGGGAGAAAAAGAAGACACTGTTGAT CCATGGCTCTGGTCCAATAATTATCCAAGGACCAAGACCATCACAGGGTCCTGGCTATGGGCTGCAGAAG AGGGAAATATAGATGATGAGACTGGAGAAAAGATTAAACTACCAACTTTAGAGGACAATGCATTCAATTC TTGGTTCTGGAAAGAAAATGAAGAAAGCATTGTAGAGGCTCCCAAGAGAGAAGAATTCAGGCCAGAAGCG GAAGAGGAAGACATTATTGGTTCTTGGTTTTGGGCTGGGGATGAAGACAGGTTTGAGCCAGCTGCTAAGA TTAATGAAGAGAACAAGATAGCATCTGAAGATGAAGATACAGTTGGATCCTGGTTCTGGGGCAATGAAGA GGCCAGTCTAGAGGCAGTGAGAAGGGGTACTTTTGAGTCTGCTCCTGGGATTAAGGAAGAGAAAGTTACT GGGTCATGGTTCTGGACTGATAAGGCCAAAGTAGGGGCTGGGTCCCAGACTGTCGAAACTGGGTCAGAAA CTGAAGAGGAAGCAATTTTTGAGTCCTTGATCTGGGCTGCAAAAAAGGACAGTATTCAAGCAGGAGTAAA GCGTGTGTCCAAGCCAAAAGATGACGGTAACATAGCTGTTGGGTCCTGGCTCTGGTCTAGTGACAAGGCC ACAAAAGAAGCTAAAACTCTGATTGTCAGTGAGGCCAGTCCAGAAAATGGGAAAGAGTCAGTTGTTAAGT TTGGGTCAAGAGCAAAAGATGAGGTGATTAACAAGACTGGCAGTGGTGACAATTGTATGCAAGGCCGTTT G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211303 protein sequence
Red=Cloning site Green=Tags(s) MTRAEVEPGAQAKAENKPGDENANAAEVEPEAPLVVRPKVRTQIMTGARPKVKPKGTPGARPKGETSTPG GAYAKCKPKAIPIARSKHDAQVWAPNKFRGESMSKMGKQCQISAADPPLLSNDSGMVAQAKCLPVDRELA NMDTESIPKKANSPAGFQPSYGSEEGTNMGSWYRARPVPKGEAYENSDFKWADKPSGSPSFWNRDEASTR FRPRKSMKANNRFRHMAKQEANTMPRHKNKQEFYNISSSDSEDESGKTPWFWPKDKTKVWSKPKEEPNSR SWFRSKKEVRVESTSGSECENPTKSLFWSGEEAKSRSKPRARKGVNMRARQQAKREACSDAMSGAIDTNK KESWFLPEEKANVFSKSKTKKEPRTRAVPKEEVKTKARASTKQEARPEEEVLVGAWFWDTQESTMADRIS IKTTFVEEEPIVGDWFWSEEEASVDSETCHTSRPRAKEEQVSSFCLGSGKKSSMESGPKATSKSMPVAKE DEVVIGSWFWADDEEINLQADDESIFGSWFWGTGENSLRSVGVNCEKMPKAGEKEVTDSWFWAGDVNTEA EVEEQARSASTKATIFVPWFWSEKQPNMDLGSEPCSDIMAGAEEEPIIGPWFWAKVDNSVEAEVNSKSSL EDEEEPIRSPWFGAREQTDMKYAAGIRYKPMAEAEDANKKSCVWAKEPCLYPTNRECLKSTLGEKEDTVD PWLWSNNYPRTKTITGSWLWAAEEGNIDDETGEKIKLPTLEDNAFNSWFWKENEESIVEAPKREEFRPEA EEEDIIGSWFWAGDEDRFEPAAKINEENKIASEDEDTVGSWFWGNEEASLEAVRRGTFESAPGIKEEKVT GSWFWTDKAKVGAGSQTVETGSETEEEAIFESLIWAAKKDSIQAGVKRVSKPKDDGNIAVGSWLWSSDKA TKEAKTLIVSEASPENGKESVVKFGSRAKDEVINKTGSGDNCMQGRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC058970 |
| ORF Size | 2871 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058970, AAH58970 |
| RefSeq Size | 4570 bp |
| RefSeq ORF | 2873 bp |
| Locus ID | 67298 |
| MW | 107.1 kDa |
| Gene Summary | Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220816 | Gprasp1 (untagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:67190 IMAGE:6826050), (10ug) |
CNY 9,120.00 |
|
| MG211303 | Gprasp1 (tGFP-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:67190 IMAGE:6826050) |
CNY 5,990.00 |
|
| MR211303L3 | Lenti ORF clone of Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:67190 IMAGE:6826050) |
CNY 7,320.00 |
|
| MR211303L4 | Lenti ORF clone of Gprasp1 (mGFP-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:67190 IMAGE:6826050) |
CNY 7,320.00 |
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