Gprasp1 (BC058970) Mouse Tagged ORF Clone

CAT#: MR211303

  • TrueORF®

Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:67190 IMAGE:6826050)

ORF Plasmid: DDK tGFP



  "BC058970" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,008.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms GASP1, GASP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTAGGGCAGAGGTTGAGCCTGGTGCCCAGGCAAAGGCTGAAAATAAGCCTGGGGATGAGAATGCTA
ATGCTGCTGAAGTAGAGCCTGAAGCCCCATTGGTGGTCAGACCCAAGGTTAGGACACAAATCATGACTGG
AGCAAGGCCCAAAGTGAAGCCTAAGGGTACCCCTGGAGCAAGACCTAAAGGTGAAACCAGTACACCAGGT
GGGGCATATGCCAAATGTAAACCTAAGGCAATCCCTATTGCTCGATCCAAGCATGATGCCCAAGTGTGGG
CCCCAAATAAGTTTCGTGGCGAGTCCATGTCAAAGATGGGAAAGCAATGTCAGATCAGTGCTGCAGACCC
TCCACTGCTCAGTAATGATTCTGGGATGGTTGCTCAAGCTAAGTGCCTGCCTGTAGATAGAGAACTTGCT
AATATGGACACTGAGAGTATTCCCAAAAAGGCTAATTCTCCAGCTGGATTCCAGCCTTCCTATGGCTCAG
AGGAGGGAACCAATATGGGGTCCTGGTACCGTGCCAGGCCTGTCCCCAAAGGAGAGGCCTATGAGAATTC
CGATTTCAAATGGGCAGATAAACCCTCAGGAAGTCCCTCCTTTTGGAATAGAGATGAAGCCAGTACAAGA
TTTCGTCCTAGGAAAAGCATGAAGGCCAATAACAGGTTCAGGCATATGGCCAAACAAGAGGCAAATACCA
TGCCTAGGCACAAAAACAAACAAGAGTTCTATAATATTTCCAGTTCTGATTCTGAGGATGAGTCTGGTAA
AACTCCCTGGTTCTGGCCCAAAGACAAGACCAAAGTCTGGTCTAAGCCCAAAGAAGAGCCCAATAGTAGG
TCCTGGTTTAGGTCTAAGAAAGAAGTCCGTGTTGAATCCACTTCTGGGTCTGAATGTGAAAATCCTACAA
AATCTTTGTTTTGGTCTGGAGAAGAGGCTAAATCTAGATCCAAACCCAGAGCCAGGAAAGGGGTCAATAT
GAGGGCCAGGCAGCAGGCCAAGCGAGAAGCTTGCAGTGATGCCATGTCTGGAGCTATAGATACAAATAAG
AAAGAGTCCTGGTTCTTGCCTGAAGAGAAAGCTAATGTTTTTTCAAAGTCTAAGACAAAGAAAGAGCCCA
GAACCAGAGCAGTGCCAAAGGAAGAAGTCAAAACTAAGGCCAGAGCAAGTACCAAGCAAGAAGCCAGGCC
AGAGGAGGAAGTCCTCGTTGGGGCTTGGTTCTGGGACACCCAGGAGTCCACTATGGCAGATAGGATAAGC
ATCAAGACCACTTTTGTAGAGGAGGAGCCCATTGTTGGAGATTGGTTCTGGAGTGAAGAAGAAGCCAGTG
TGGACTCTGAGACCTGTCATACATCCAGACCCAGGGCTAAGGAGGAGCAAGTTAGTAGTTTCTGTTTAGG
GTCTGGAAAGAAGAGCAGTATGGAAAGTGGGCCAAAGGCTACTTCTAAATCTATGCCAGTAGCTAAGGAG
GATGAGGTCGTTATTGGGTCCTGGTTCTGGGCAGATGATGAAGAGATCAACTTACAGGCTGATGACGAGT
CTATTTTTGGATCTTGGTTCTGGGGCACTGGTGAGAACAGCCTTAGATCTGTTGGAGTCAATTGTGAGAA
GATGCCGAAGGCTGGAGAAAAAGAAGTTACTGATTCCTGGTTCTGGGCTGGAGATGTCAATACAGAGGCT
GAGGTTGAAGAACAGGCCAGGTCGGCATCTACAAAAGCAACAATCTTTGTGCCTTGGTTTTGGTCTGAAA
AGCAGCCGAACATGGATTTAGGGTCTGAACCTTGCTCAGATATCATGGCAGGAGCTGAGGAGGAGCCCAT
AATTGGGCCCTGGTTCTGGGCTAAAGTAGATAATAGTGTGGAGGCTGAAGTTAACAGTAAGTCTAGCCTT
GAGGATGAGGAAGAACCCATTAGATCCCCTTGGTTTGGGGCCAGAGAACAAACCGATATGAAGTATGCAG
CTGGTATCAGATACAAGCCTATGGCAGAAGCTGAGGATGCTAACAAAAAGTCTTGTGTCTGGGCAAAAGA
ACCCTGTTTGTATCCTACCAATAGAGAATGCTTGAAATCTACTCTGGGAGAAAAAGAAGACACTGTTGAT
CCATGGCTCTGGTCCAATAATTATCCAAGGACCAAGACCATCACAGGGTCCTGGCTATGGGCTGCAGAAG
AGGGAAATATAGATGATGAGACTGGAGAAAAGATTAAACTACCAACTTTAGAGGACAATGCATTCAATTC
TTGGTTCTGGAAAGAAAATGAAGAAAGCATTGTAGAGGCTCCCAAGAGAGAAGAATTCAGGCCAGAAGCG
GAAGAGGAAGACATTATTGGTTCTTGGTTTTGGGCTGGGGATGAAGACAGGTTTGAGCCAGCTGCTAAGA
TTAATGAAGAGAACAAGATAGCATCTGAAGATGAAGATACAGTTGGATCCTGGTTCTGGGGCAATGAAGA
GGCCAGTCTAGAGGCAGTGAGAAGGGGTACTTTTGAGTCTGCTCCTGGGATTAAGGAAGAGAAAGTTACT
GGGTCATGGTTCTGGACTGATAAGGCCAAAGTAGGGGCTGGGTCCCAGACTGTCGAAACTGGGTCAGAAA
CTGAAGAGGAAGCAATTTTTGAGTCCTTGATCTGGGCTGCAAAAAAGGACAGTATTCAAGCAGGAGTAAA
GCGTGTGTCCAAGCCAAAAGATGACGGTAACATAGCTGTTGGGTCCTGGCTCTGGTCTAGTGACAAGGCC
ACAAAAGAAGCTAAAACTCTGATTGTCAGTGAGGCCAGTCCAGAAAATGGGAAAGAGTCAGTTGTTAAGT
TTGGGTCAAGAGCAAAAGATGAGGTGATTAACAAGACTGGCAGTGGTGACAATTGTATGCAAGGCCGTTT
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211303 protein sequence
Red=Cloning site Green=Tags(s)

MTRAEVEPGAQAKAENKPGDENANAAEVEPEAPLVVRPKVRTQIMTGARPKVKPKGTPGARPKGETSTPG
GAYAKCKPKAIPIARSKHDAQVWAPNKFRGESMSKMGKQCQISAADPPLLSNDSGMVAQAKCLPVDRELA
NMDTESIPKKANSPAGFQPSYGSEEGTNMGSWYRARPVPKGEAYENSDFKWADKPSGSPSFWNRDEASTR
FRPRKSMKANNRFRHMAKQEANTMPRHKNKQEFYNISSSDSEDESGKTPWFWPKDKTKVWSKPKEEPNSR
SWFRSKKEVRVESTSGSECENPTKSLFWSGEEAKSRSKPRARKGVNMRARQQAKREACSDAMSGAIDTNK
KESWFLPEEKANVFSKSKTKKEPRTRAVPKEEVKTKARASTKQEARPEEEVLVGAWFWDTQESTMADRIS
IKTTFVEEEPIVGDWFWSEEEASVDSETCHTSRPRAKEEQVSSFCLGSGKKSSMESGPKATSKSMPVAKE
DEVVIGSWFWADDEEINLQADDESIFGSWFWGTGENSLRSVGVNCEKMPKAGEKEVTDSWFWAGDVNTEA
EVEEQARSASTKATIFVPWFWSEKQPNMDLGSEPCSDIMAGAEEEPIIGPWFWAKVDNSVEAEVNSKSSL
EDEEEPIRSPWFGAREQTDMKYAAGIRYKPMAEAEDANKKSCVWAKEPCLYPTNRECLKSTLGEKEDTVD
PWLWSNNYPRTKTITGSWLWAAEEGNIDDETGEKIKLPTLEDNAFNSWFWKENEESIVEAPKREEFRPEA
EEEDIIGSWFWAGDEDRFEPAAKINEENKIASEDEDTVGSWFWGNEEASLEAVRRGTFESAPGIKEEKVT
GSWFWTDKAKVGAGSQTVETGSETEEEAIFESLIWAAKKDSIQAGVKRVSKPKDDGNIAVGSWLWSSDKA
TKEAKTLIVSEASPENGKESVVKFGSRAKDEVINKTGSGDNCMQGRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC058970
ORF Size 2871 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058970, AAH58970
RefSeq Size 4570 bp
RefSeq ORF 2873 bp
Locus ID 67298
MW 107.1 kDa
Gene Summary Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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