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Diap3 (BC028920) Mouse Tagged ORF Clone
CAT＃: MR211218
- TrueORF®
Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236)
ORF Plasmid: tGFP
"BC028920" in other vectors (4)
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CNY 6,808.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dia2, mDia2, p134MDia2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211218 representing BC028920
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGATCCCAGGCAGCCCGCTATGATGGCAG ACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAAAGCATCCTTGAAGAAGTGTTAGAAGC CTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTTCCATTGTGGAAGGCCTCCGGCATAAC TCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCTCGTTACATCTCCTGATGATTTGGACT TCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAACTTAAAGGGCAT TAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGCACAAAGAAGAAGATTTGAGTGAGTTT TTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATCTGATGTTTACAGCATGTTATGGGACA CAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTTCAGCATCTCCTGCTCATTCGCAATGA TCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTGTGTCACAGATTGTGTTACATAGAGAT GGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGACTTAAGTCAGTTTGTAGATGTTTGCA TAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAACATTGCAAGAAATTTGAAAAAGAGTG TACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGGCAAAGATTAATGAGCTTCAAGCAGAG TTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTACAAAAATTCCTTTGCAACCTTCAGTAG AAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCACTCAGTGGAGGAGTGCCGCCTCCCCC ACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAATGCCATTTGGTGGCCCTGTACCACCA CCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATTAAACCTGCCATTTGGTTTGAAACCAA AGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCGGACCAAATGAAATGTC TGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAAATAGGGATTTGCTTTGTAAACTTGAG AACACTTTTTGTTGCCAAGACAAAGAGAAAAGGAATACAAATGACTTTGATGAGAAGAAAGTTATTAAGA AGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAGAACCTTTCAATCTTCCTGAGCTCCTT CCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGGATGAAACACAGTTGTCAGAGTCCATG ATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAAGTCATTGTCCCAGTTTAGAAGTGACT ATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGCAATGTGAAGAGACTCCGGCCACGGCT CAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC AGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAAGTTGCTGGAACTTGTGTTGCTAATGG GAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTGACCTTAGCTCTCTCTGTAAACT GAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATTTCCTGGTAGATGTATGTGAAGAAAAG CATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGACAAAGCTAGCAGAGTCTCTGTAGAAA TGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGAATTTGGAAACCTTTCC CCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCAGCTTCGTTATCAGTGCGAACGAGCAG TATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTACCAGAGTATAATGGGCTACTATGCTG TCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTGAACAACTTCAGAACTTCATTTATGCT AGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGGAGAAACGTGCCAGGATAGCGAAAGAG CGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCGCTTACTAGAAATGAAAACTGAGGGAG ATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCAGGGGCTGCCTTCCGCGACAGAAGAAA AAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGATGTCTCAGAGGCCTGTTCTCAAAGTT TGTAACCATGAAAATCAGAAAATGCAGTTGACAGAAGGGTCACGTCCACACCACAGTATCAATTGCAACT CCACCAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGACACTCATGCGTCTACAGGGAGGATCAA GGCAGTTGAGAAGGAAGCCTGTAATGCAGAAAGCAACAGAAAAAAGGAAATGGAACTTCTTGGCTCTGTT GCTAAAAGCGAATCAGTTCCTGAAGTTGAAGCCCTGCTGGCAAGATTACGAGCTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211218 representing BC028920
Red=Cloning site Green=Tags(s) MSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHN SVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEF FHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRD GTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAE LQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPP PPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLE NTFCCQDKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESM IQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAV STACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEK HADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQ YEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKE RAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQSGAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKV CNHENQKMQLTEGSRPHHSINCNSTRTPVAKELNYNLDTHASTGRIKAVEKEACNAESNRKKEMELLGSV AKSESVPEVEALLARLRAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC028920 |
| ORF Size | 2787 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC028920.1 |
| RefSeq Size | 3770 bp |
| RefSeq ORF | 2789 bp |
| Locus ID | 56419 |
| MW | 138.2 kDa |
| Gene Summary | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220799 | Diap3 (untagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236), (10ug) |
CNY 9,120.00 |
|
| MG211218 | Diap3 (tGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) |
CNY 5,890.00 |
|
| MR211218L3 | Lenti ORF clone of Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) |
CNY 7,220.00 |
|
| MR211218L4 | Lenti ORF clone of Diap3 (mGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) |
CNY 7,220.00 |
