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Gnptab (BC060638) Mouse Tagged ORF Clone
CAT＃: MR211191
- TrueORF®
Gnptab (Myc-DDK-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (cDNA clone MGC:79224 IMAGE:6849405), complete
ORF Plasmid: tGFP
"BC060638" in other vectors (4)
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CNY 9,220.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EG432486; mKIAA1208 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211191 representing BC060638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGAAACCCTCGGGAAGAACACAACCGAACCGACAAAGAAGAGTGAGAAGCAGCTGGAATGTCTGC TGACGCACTGCATTAAGGTGCCCATGCTTGTTCTGGACCCGCCCCTGCCAGCCAACTGCACCCTGAAGGA TCTGCCAACCCTTTATCCATCTTTCCACGCTGCCAGCGACATGTTCAATGTTGCGAAACCAAAAAATCCG TCTACTAATGTCTCCGTTGTCGTTTTTGACACTACTAAGGATGTTGAAGACGCCCATGCTGGACCGTTTA AGGGAGGCAGCAAACAGATGGTTTGGAGAGCCTACTTGACAACAGACAAAGAAGCCCCTGGCTTAGTGCT GATGCAAGGCTTGGCGTTCCTGAGTGGATTCCCACCGACCTTCAAGGAGACGAGTCAACTGAAGACAAAG CTGCCAGAAAAGCTTTCCTCTAAAATAAAGCTGTTGCGGCTGTACTCGGAGGCCAGTGTCGCTCTTCTGA AATTGAATAATCCCAAGGGTTTCCAAGAGCTGAACAAGCAGACCAAGAAGAACATGACCATCGATGGGAA GGAACTGACCATCAGCCCTGCGTATCTGCTGTGGGACCTGAGTGCCATCAGCCAGTCCAAGCAGGATGAG GACGTGTCTGCCAGCCGCTTTGAGGATAATGAAGAGCTGAGGTACTCGCTGCGATCTATCGAGAGACACG CGCCATGGGTACGGAATATTTTCATTGTCACCAACGGGCAGATTCCATCCTGGCTGAACCTTGACAACCC TCGAGTGACCATAGTGACCCACCAGGACATTTTCCAAAATCTGAGCCACTTGCCTACTTTCAGTTCCCCT GCTATTGAAAGTCACATTCACCGCATCGAAGGGCTGTCCCAGAAGTTTATTTATCTAAATGACGATGTCA TGTTCGGTAAGGACGTCTGGCCGGACGATTTTTACAGCCACTCCAAAGGTCAAAAGGTTTATTTGACATG GCCTGTGCCAAACTGTGCAGAGGGCTGCCCGGGCTCCTGGATTAAAGACGGCTATTGTGATAAGGCCTGT AATAACTCAGCCTGTGACTGGGATGGCGGAGACTGCTCTGGTAATACTGCAGGGAACCGGTTTGTTGCAG GAGGTGGGGGTACCGGGAATATTGGAGCTGGACAGCACTGGCAGTTTGGTGGAGGAATAAACACCATCTC TTACTGTAACCAAGGATGTGCAAACTCCTGGCTGGCTGACAAGTTCTGTGACCAAGCCTGTAACGTCTTA TCCTGCGGGTTTGATGCTGGTGACTGTGGACAAGATCATTTTCATGAATTGTATAAAGTAACACTTCTCC CAAACCAGACTCACTATGTTGTCCCCAAAGGTGAATACCTGTCTTATTTCAGCTTTGCTGGTGAACACAG ATCAGAGAGATGGACTGCCCCAGCAGAGACAGTGACCGTGAAAGGCCGTGACCACGCTTTGAATCCACCC CCGGTGTTGGAGACCAATGCAAGATTGGCCCAGCCTACACTAGGCGTGACTGTGTCCAAAGAGAACCTTT CACCGCTGATCGTTCCCCCAGAAAGCCACTTGCCAAAAGAAGAGGAGAGTGACAGGGCAGAAGGCAATGC TGTACCTGTAAAGGAGTTAGTGCCTGGCAGACGGTTGCAGCAGAATTATCCAGGCTTTTTGCCCTGGGAG AAAAAAAAGTATTTCCAAGACCTTCTTGATGAGGAAGAGTCATTGAAGACCCAGTTGGCGTACTTTACAG ACAGCAAACATACCGGGAGGCAACTAAAAGATACATTTGCAGACTCCCTCCGATACGTCAATAAAATTCT CAACAGCAAGTTTGGATTCACATCCAGGAAAGTCCCTGCACACATGCCGCACATGATTGACAGGATCGTT ATGCAAGAACTCCAAGATATGTTCCCTGAAGAATTTGACAAGACTTCATTTCACAAGGTGCGTCACTCTG AGGACATGCAGTTTGCCTTCTCCTACTTTTATTACCTCATGAGTGCAGTTCAGCCCCTCAATATTTCCCA AGTCTTTCATGAAGTAGACACAGACCAATCTGGTGTCTTGTCTGATAGGGAAATCCGAACACTGGCCACG AGAATTCACGACCTACCTTTAAGCTTGCAGGATTTGACAGGTTTGGAACACATGTTAATAAATTGCTCAA AAATGCTCCCCGCTAATATCACTCAACTCAACAACATCCCACCGACTCAGGAAGCATACTACGACCCCAA CCTGCCTCCGGTCACTAAGAGTCTTGTCACCAACTGTAAGCCAGTAACTGACAAGATCCACAAAGCCTAT AAAGACAAGAACAAATACAGGTTTGAAATCATGGGAGAGGAAGAAATCGCTTTCAAGATGATACGAACCA ATGTTTCTCATGTGGTTGGCCAGTTGGATGACATCAGAAAAAACCCCAGGAAGTTCGTTTGTCTGAATGA CAACATTGACCACAACCATAAAGATGCCCGGACAGTGAAGGCTGTCCTCAGGGACTTCTATGAGTCCATG TTTCCCATACCTTCCCAGTTTGAGCTGCCAAGAGAGTATCGGAACCGCTTTCTGCACATGCATGAGCTCC AAGAATGGCGGGCATATCGAGACAAGCTGAAGTTTTGGACCCACTGCGTACTAGCAACGTTGATTATATT TACTATATTCTCATTTTTTGCTGAACAGATAATTGCTCTGAAGCGAAAGATATTTCCCAGGAGGAGGATA CACAAAGAAGCTAGTCCAGACCGAATCAGGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211191 representing BC060638
Red=Cloning site Green=Tags(s) MRETLGKNTTEPTKKSEKQLECLLTHCIKVPMLVLDPPLPANCTLKDLPTLYPSFHAASDMFNVAKPKNP STNVSVVVFDTTKDVEDAHAGPFKGGSKQMVWRAYLTTDKEAPGLVLMQGLAFLSGFPPTFKETSQLKTK LPEKLSSKIKLLRLYSEASVALLKLNNPKGFQELNKQTKKNMTIDGKELTISPAYLLWDLSAISQSKQDE DVSASRFEDNEELRYSLRSIERHAPWVRNIFIVTNGQIPSWLNLDNPRVTIVTHQDIFQNLSHLPTFSSP AIESHIHRIEGLSQKFIYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKAC NNSACDWDGGDCSGNTAGNRFVAGGGGTGNIGAGQHWQFGGGINTISYCNQGCANSWLADKFCDQACNVL SCGFDAGDCGQDHFHELYKVTLLPNQTHYVVPKGEYLSYFSFAGEHRSERWTAPAETVTVKGRDHALNPP PVLETNARLAQPTLGVTVSKENLSPLIVPPESHLPKEEESDRAEGNAVPVKELVPGRRLQQNYPGFLPWE KKKYFQDLLDEEESLKTQLAYFTDSKHTGRQLKDTFADSLRYVNKILNSKFGFTSRKVPAHMPHMIDRIV MQELQDMFPEEFDKTSFHKVRHSEDMQFAFSYFYYLMSAVQPLNISQVFHEVDTDQSGVLSDREIRTLAT RIHDLPLSLQDLTGLEHMLINCSKMLPANITQLNNIPPTQEAYYDPNLPPVTKSLVTNCKPVTDKIHKAY KDKNKYRFEIMGEEEIAFKMIRTNVSHVVGQLDDIRKNPRKFVCLNDNIDHNHKDARTVKAVLRDFYESM FPIPSQFELPREYRNRFLHMHELQEWRAYRDKLKFWTHCVLATLIIFTIFSFFAEQIIALKRKIFPRRRI HKEASPDRIRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060638 |
| ORF Size | 2763 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060638.1 |
| RefSeq Size | 4699 bp |
| RefSeq ORF | 2765 bp |
| Locus ID | 432486 |
| MW | 172.3 kDa |
| Gene Summary | Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220959 | Gnptab (untagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (cDNA clone MGC:79224 IMAGE:6849405), complete, (10ug) |
CNY 9,120.00 |
|
| MG211191 | Gnptab (tGFP-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (cDNA clone MGC:79224 IMAGE:6849405), complete |
CNY 10,170.00 |
|
| MR211191L3 | Lenti ORF clone of Gnptab (Myc-DDK-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (cDNA clone MGC:79224 IMAGE:6849405), complete |
CNY 11,120.00 |
|
| MR211191L4 | Lenti ORF clone of Gnptab (mGFP-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (cDNA clone MGC:79224 IMAGE:6849405), complete |
CNY 11,120.00 |
