Clca2 (BC008147) Mouse Tagged ORF Clone

CAT#: MR211116

  • TrueORF®

Clca2 (Myc-DDK-tagged) - Mouse chloride channel calcium activated 2 (cDNA clone MGC:5738 IMAGE:3583804)

ORF Plasmid: DDK tGFP



  "BC008147" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 6,608.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Clca2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211116 representing BC008147
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCAGGGCTGCAGGTCCTTCTGTTCCTCACCCTGCATCTCCTGCAGAACACAGAGAGCTCCATGG
TGCATCTCAACAGCAATGGATACGAGGGTGTGGTCATTGCCATTAACCCCAGTGTGCCAGAGGACGAAAG
GCTCATCCCAAGCATAAAGGAAATGGTAACTCAAGCTTCTACCTACCTGTTTGAAGCCAGCCAAGGAAGA
GTTTATTTCAGGAACATAAGCATATTAGTCCCGATGACCTGGAAGTCAAAATCTGAGTACTTAATGCCAA
AACGAGAATCGTACGACAAAGCAGACGTCATAGTTGCGGATCCTCACCTGCAACATGGAGACGACCCCTA
CACCCTTCAGTATGGACAGTGTGGGGACAGAGGACAGTACATACACTTCACTCCAAACTTCCTACTCACT
GATAACTTGCGTATCTATGGACCCCGAGGCAGAGTCTTTGTCCATGAGTGGGCCCATCTCCGGTGGGGAG
TATTTGATGAGTATAACGTGGACCGGCCTTTCTACATTTCTAGAAAGAACACTATAGAAGCAACAAGGTG
CTCCGCCAGCATCACAGGCAAGAAGGTGGTCCACGAGTGTCAGAGAGGCAGCTGTGTGACAAGGGCGTGC
CGGCGTGACTCGAAGACACGGCTGTATGAACCCAAATGTACATTTATCCCAGACAAAATACAGACAGCTG
GGGCCTCCATAATGTTCATGCAAAACCTCAATTCTGTGGTTGAATTTTGCACAGAAAATAACCACAATGC
AGAAGCCCCAAACCTACAAAACAAAATGTGCAATCGCAGAAGCACGTGGGATGTAATCAAGGCGTCTGCT
GACTTTCAGAATTCCCCTCCCATGAGAGGAACAGAAGCGCCTCCTCCACCTACATTTTCATTGCTCAAGT
CCAGAAGGCGAGTGGTGTGCTTGGTGCTGGATAAATCTGGAAGCATGGACAAAGAAGACCGTCTTATTCG
AATGAATCAAGCAGCAGAACTGTACTTAACTCAAATTGTGGAAAAGGAGTCTATGGTTGGATTAGTCACA
TTTGACAGCGCTGCCCACATCCAAAATTATCTAATAAAAATAACGAGTAGTAGTGACTACCAAAAGATCA
CCGCAAACCTCCCCCAACAGGCTACTGGTGGAACTTCAATTTGCCATGGACTCCAGGCAGGATTTCAGGC
AATTACCTCCAGTGACCAGAGCACTTCCGGTTCTGAGATCGTATTGCTGACAGATGGGGAAGATAATGGA
ATAAGTTCCTGCTTTGAGGCCGTCTCTCGCAGCGGTGCCATCATCCACACCATCGCTCTGGGGCCTTCTG
CTGCCCGAGAACTGGAGACTCTGTCAGACATGACAGGAGGGCTTCGTTTCTATGCCAACAAACATGTAAG
CAGCCTTATCGACGCTTTCAGTAGGATTTCATCTACAAGTGGCAGCGTCTCCCAGCAGGCTCTGCAGTTG
GAGAGCAAAGCCTTCAATGTCAGAGCAGGGGCATGGATAAACAGTACAGTACCTGTGGACAGTACCGTCG
GCAACGACACGTTCTTTGTTATCACCTGGACGGTACAAAAGCCAGAAATCATTCTTCAAGATCCAAAAGG
AAAAAAATATATAACCTCAGATTTCCAAGATGATGAACTAAACATCCGGTCTGCTCGACTTCAAATACCG
GGCACTGCAGAGACAGGTACTTGGACTTACAGCATCACGGGTACCAAGTCTCAGTTGATTACAATGACAG
TGACCACTCGAGCAAGAAGTCCCACCATGGAACCACTCCTGGCTACCGCTCACATGAGTCAGAGCACAGC
CCAGTACCCTAGCCGGATGATTGTGTATGTACGGGTCAGCCAAGGGTTTTTGCCTGTTCTAGGAGCCAAT
GTCACAGCCATCATAGAAGCTGAACATGGACATCAAGTCACCCTGGAGCTATGGGACAATGGGGCAGGTG
CTGATACTGTTAAAAATGATGGCATCTACACAAGATACTTTACAGATTATCATGGAAATGGTAGATACAG
CCTAAAAGTGCGTGTCCAGGCAAGAAAAAACAAAGCCAGACTAAGCTTAAGACAGAAGAACAAGTCTTTA
TATATACCTGGCTATGTGGAAAATGGTAAAATTGTACTGAATCCACCCAGACCAGATGTCCAAGAAGAAG
CCATAGAAGCTACAGTGGAGGACTTCAACAGAGTAACCTCTGGAGGGTCATTTACTGTGTCTGGAGCGCC
CCCTGATGGCGACCATGCTCGTGTGTTCCCACCAAGTAAAGTCACAGACCTGGAGGCTGAGTTTATAGGT
GATTATATTCAACTTACATGGACGGCCCCTGGCAAGGTTCTCGATAAGGGAAGAGCGCATAGATACATCA
TCAGAGTGAGCCAGCATCCTCTGGGTCTCCAAGAAGATTTTAACAATGCTACTTTAGTGAATGCTTCCAG
TCTGATACCTAAAGAGGCTGGCTCAAAAGAAACATTTAAATTCAAACCAGAAACTTTTAAAATAGCAAAT
GACACCCAGCTCTACATTGCAATCCAGGCATACAATGAAGCTGGTCTCACCTCTGAGGTCTCCAACATTG
CACAGGCTGTCAAGTTTACTTCTCTAGAAGATAGTATCTCTGCACTGGGTGCTGATATTTCTGCAATCTC
TATGACAGTTTGGGGGTTAGCTGTGATTTTTAACTCTATTTTAAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211116 representing BC008147
Red=Cloning site Green=Tags(s)

MVPGLQVLLFLTLHLLQNTESSMVHLNSNGYEGVVIAINPSVPEDERLIPSIKEMVTQASTYLFEASQGR
VYFRNISILVPMTWKSKSEYLMPKRESYDKADVIVADPHLQHGDDPYTLQYGQCGDRGQYIHFTPNFLLT
DNLRIYGPRGRVFVHEWAHLRWGVFDEYNVDRPFYISRKNTIEATRCSASITGKKVVHECQRGSCVTRAC
RRDSKTRLYEPKCTFIPDKIQTAGASIMFMQNLNSVVEFCTENNHNAEAPNLQNKMCNRRSTWDVIKASA
DFQNSPPMRGTEAPPPPTFSLLKSRRRVVCLVLDKSGSMDKEDRLIRMNQAAELYLTQIVEKESMVGLVT
FDSAAHIQNYLIKITSSSDYQKITANLPQQATGGTSICHGLQAGFQAITSSDQSTSGSEIVLLTDGEDNG
ISSCFEAVSRSGAIIHTIALGPSAARELETLSDMTGGLRFYANKHVSSLIDAFSRISSTSGSVSQQALQL
ESKAFNVRAGAWINSTVPVDSTVGNDTFFVITWTVQKPEIILQDPKGKKYITSDFQDDELNIRSARLQIP
GTAETGTWTYSITGTKSQLITMTVTTRARSPTMEPLLATAHMSQSTAQYPSRMIVYVRVSQGFLPVLGAN
VTAIIEAEHGHQVTLELWDNGAGADTVKNDGIYTRYFTDYHGNGRYSLKVRVQARKNKARLSLRQKNKSL
YIPGYVENGKIVLNPPRPDVQEEAIEATVEDFNRVTSGGSFTVSGAPPDGDHARVFPPSKVTDLEAEFIG
DYIQLTWTAPGKVLDKGRAHRYIIRVSQHPLGLQEDFNNATLVNASSLIPKEAGSKETFKFKPETFKIAN
DTQLYIAIQAYNEAGLTSEVSNIAQAVKFTSLEDSISALGADISAISMTVWGLAVIFNSILN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC008147
ORF Size 2706 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC008147.1
RefSeq Size 3137 bp
RefSeq ORF 2708 bp
Locus ID 80797
MW 99.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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