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Wwp2 (NM_025830) Mouse Tagged ORF Clone
CAT＃: MR211018
- TrueORF®
Wwp2 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2)
ORF Plasmid: tGFP
"NM_025830" in other vectors (4)
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CNY 6,376.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300010O06Rik; AA690238; AIP2; AW554328 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211018 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCTGCCAGCTCCAGCCGGGCAGGAGTGGCCCTGCCTTTTGAGAAGTCCCAGCTTACCCTGAAAG TGGTGTCAGCAAAGCCCAAGGTGCACAACCGCCAGCCCAGAATCAACTCCTACGTGGAGGTGGCAGTGGA TGGACTCCCCAGCGAGACCAAGAAGACGGGGAAGCGCATCGGGAGCTCTGAACTGCTCTGGAATGAAATC ATCGTTCTGAATGTCACAGCCCAGAGTCATTTGGATCTGAAGGTCTGGAGCTGTCATACCTTGAGGAATG AACTACTGGGCACTGCCTCTGTCAACCTCTCCAATGTCCTGAAGAACAATGGCGGCAAAATGGAGAACAC ACAACTGACCCTGAACCTGCAGACAGAGAACAAAGGCAGTGTTGTCTCGGGAGGAGAGCTGACAATTTTC CTGGATGGGCCAACTGTTGATCTGGGAAGTGTGCCTAATGGCAGTGCAGTGACAGACGGATCACAGCCAC CTTCAAGAGAATCCAGTGGGACTGCTATAGCTCCAGAGACCCGGCACCAGCCCCCCAGTACAAACTGCTT TGGTGGCAGATCCCGGACGCACAGACACTCAGGTGGCTCAGCCAGGACAGCCACAGCAGCCAGTGAACAA AGCCCTGGCGCTAGGAACCGCCACCGCCAGCCTGTGAAGAACTCTAGCAGCAGTGGCTTAGCCAATGGCA CAGTGAATGAGGAACCTACTCCAGCCAGTGAACCTGAAGAATCGTCGGTTGTTGGTGTAACATCCCTGCC TGCAGCAGCCTTGAGTGTGTCCTCAAATCCCAACACAACATCTCTCCCTGCACAGTCCACACCAGCAGAG GGAGAGGAGGCCAGCACTTCCGGGACACAGCAGCTCCCTGCTGCCGCCCAGGCCCCTGATGCTCTTCCTG CTGGATGGGAACAGAGAGAGCTGCCCAATGGGCGTGTCTATTATGTTGATCACAACACCAAGACCACCAC CTGGGAGCGGCCTCTTCCTCCAGGGTGGGAAAAGCGCACGGACCCCCGAGGGAGGTTTTACTACGTGGAC CACAACACCCGGACAACCACCTGGCAGCGCCCAACTGCTGAGTACGTGCGCAACTATGAGCAGTGGCAGT CCCAGCGGAACCAGCTGCAGGGGGCCATGCAGCACTTCAGCCAAAGATTCCTCTACCAGTCTTCGAGTGC TTCGACTGACCATGATCCCTTGGGCCCCCTCCCACCTGGCTGGGAGAAGAGGCAGGACAATGGACGGGTG TATTATGTCAACCACAACACTCGCACTACCCAGTGGGAGGACCCTCGGACCCAGGGGATGATACAGGAGC CAGCCCTGCCCCCAGGGTGGGAGATGAAATACACCAGCGAGGGCGTGCGGTACTTTGTGGACCACAATAC CCGCACCACTACCTTTAAGGATCCTCGCCCAGGGTTCGAGTCAGGGACAAAGCAAGGCTCACCTGGTGCC TATGACCGAAGTTTTCGGTGGAAGTATCACCAGTTCCGTTTCCTCTGCCACTCAAATGCTCTACCCAGCC ATGTGAAGATCAGCGTTTCCAGGCAGACACTCTTTGAGGATTCTTTCCAACAGATTATGAACATGAAACC TTACGACCTGCGCCGCCGGCTCTACATCATCATGCGTGGTGAGGAGGGCCTGGACTACGGCGGCATCGCC AGAGAGTGGTTTTTCCTCCTGTCCCATGAGGTGCTCAACCCTATGTACTGTTTGTTTGAATATGCTGGGA AGAACAATTACTGCCTGCAGATCAACCCGGCCTCTTCCATCAACCCTGACCACCTCACCTACTTCCGCTT TATCGGCAGATTCATCGCCATGGCTCTGTACCATGGGAAGTTCATCGACACAGGCTTCACTCTCCCTTTC TACAAGCGGATGCTCAACAAGAGACCGACTCTGAAGGACCTGGAGTCTATTGACCCTGAGTTTTACAACT CCATTGTCTGGATCAAAGAGAACAACCTGGAAGAGTGTGGTCTGGAGCTGTTTTTCATCCAGGACATGGA GATTCTGGGCAAGGTGACAACCCATGAACTGAAGGAGGGCGGTGAGAACATCCGAGTTACCGAGGAGAAC AAGGAGGAGTATATCATGCTGCTGACTGACTGGCGATTCACCCGAGGCGTGGAAGAGCAGACCAAAGCTT TCCTGGATGGCTTCAATGAGGTTGCCCCTCTGGAGTGGTTGAGATATTTTGATGAGAAAGAGCTGGAGCT CATGCTCTGCGGCATGCAGGAGATAGACATGAGCGACTGGCAGAAGAACGCCATCTATCGGCACTACACC AAGAGCAGCAAGCAGATCCAGTGGTTCTGGCAGGTTGTCAAGGAGATGGACAATGAGAAGAGGATCCGGC TACTGCAGTTTGTCACGGGAACCTGCCGTCTGCCTGTTGGGGGATTTGCTGAGCTCATCGGGAGCAATGG CCCGCAGAAGTTCTGCATCGACAGAGTTGGCAAGGAAACCTGGCTGCCCAGGAGCCATACGTGCTTCAAC CGTCTGGACCTGCCTCCCTATAAGAGCTACGAGCAGCTGAAAGAGAAGCTGCTGTACGCCATCGAGGAGA CTGAGGGGTTCGGACAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211018 protein sequence
Red=Cloning site Green=Tags(s) MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEI IVLNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENTQLTLNLQTENKGSVVSGGELTIF LDGPTVDLGSVPNGSAVTDGSQPPSRESSGTAIAPETRHQPPSTNCFGGRSRTHRHSGGSARTATAASEQ SPGARNRHRQPVKNSSSSGLANGTVNEEPTPASEPEESSVVGVTSLPAAALSVSSNPNTTSLPAQSTPAE GEEASTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVD HNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRV YYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGA YDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIA REWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPF YKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELFFIQDMEILGKVTTHELKEGGENIRVTEEN KEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKNAIYRHYT KSSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDRVGKETWLPRSHTCFN RLDLPPYKSYEQLKEKLLYAIEETEGFGQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025830 |
| ORF Size | 2613 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025830.4 |
| RefSeq Size | 4319 bp |
| RefSeq ORF | 2613 bp |
| Locus ID | 66894 |
| UniProt ID | Q9DBH0 |
| MW | 98.8 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; regulates POU5F1 protein level during differentiation of embryonal carcinoma cells (ECCs) but not in undifferentiated ECCs and embryonic stem cells (ESCs). Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202685 | Wwp2 (untagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2), (10ug) |
CNY 2,000.00 |
|
| MG211018 | Wwp2 (tGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) |
CNY 7,890.00 |
|
| MR211018L3 | Lenti ORF clone of Wwp2 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) |
CNY 9,030.00 |
|
| MR211018L4 | Lenti ORF clone of Wwp2 (mGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) |
CNY 9,030.00 |
