Pik3c3 (BC024675) Mouse Tagged ORF Clone

CAT#: MR210937

  • TrueORF®

Pik3c3 (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase, class 3 (cDNA clone MGC:30377 IMAGE:3661094)

ORF Plasmid: DDK tGFP



  "BC024675" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,208.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 5330434F23, Vps34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210937 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGGCGGAGAAGTTCCACTACATCTACAGCTGTGACCTGGACATCAACGTGCAGCTGAAGATAG
GGAGCTTGGAAGGGAAGAGAGAACAAAAGAGCTATAAAGCTGTCCTAGAAGATCCCATGTTAAAGTTTTC
TGGGCTATACCAAGAGACATGCTCCGACCTCTATGTGACTTGTCAAGTGTTTGCTGAAGGGAAGCCCCTG
GCCTTACCAGTCAGAACATCCTACAAGGCGTTTAGTACAAGATGGAATTGGAACGAGTGGCTGAAACTTC
CTGTCAAGTACCCTGACCTGCCCAGGAATGCCCAAGTGGCCTTGACTATATGGGATGTGTATGGACCGGG
AAGCGCTGTGCCAGTGGGAGGAACAACCGTGTCGCTCTTTGGAAAATATGGCATGTTTCGCCAAGGAATG
CATGACTTGAAGGTCTGGCCTAACGTGGAGGCAGATGGTTCTGAACCTACAAGAACTCCCGGCAGAACAA
GCAGCACACTGTCAGAAGATCAGATGAGCCGCCTCGCCAAGCTCACCAAGGCTCATCGGCAAGGACACAT
GGTGAAAGTGGATTGGCTGGACAGATTAACATTTAGAGAGATAGAAATGATAAATGAGAGTGAGAAACGA
AGCTCTAATTTCATGTACTTGATGGTTGAGTTTCGCTGTGTAAAGTGCGATGACAAGGAGTATGGCATTG
TTTACTATGAAAAGGATGGTGACGAATCATCTCCAATTTTAACCAGTTTTGAGTTAGTGAAAGTTCCTGA
TCCTCAAATGTCTATGGAGAATTTAGTGGAGAGCAAACACCACAAGCTTGCTCGGAGCTTAAGAAGCGGA
CCATCTGACCACGATCTCAAACCCAACGCTACCACAAGAGATCAGCTAAATATTATTGTGAGTTACCCAC
CAACCAAGCAACTCACATATGAAGAACAAGATCTTGTTTGGAAATTTAGATATTATCTTACTAATCAAGA
AAAAGCTCTGACGAAGTTCTTGAAGTGTGTTAATTGGGACCTGCCCCAGGAGGCCAAGCAGGCCCTGGAA
CTTCTGGGAAAGTGGAAGCCAATGGATGTAGAGGACTCCCTGGAGCTGCTCTCCTCTCATTACACCAACC
CCACCGTGAGGCGCTACGCTGTGGCCCGCCTGAGGCAGGCCGATGATGAGGATTTGCTGATGTACTTACT
GCAGCTGGTCCAGGCTCTGAAATATGAAAACTTTGATGACATAAAGAATGGTTTGGAACCTACGAAGAAG
GATAGTCAAACTTCAGCATCAGAAAGTCTGTCCAATTCTGGAGTCAGTTCTGGAGACATAGATAGCTCCC
AAATTATAACCAACCCTCTTCCTCCCGTGGCCCCCCCTCCTGCATCTAAAGCAAAGGAAGTTTCGGATGG
GGAGAGTCTCGAGCAAGATCTATGTACGTTCTTGATATCAAGAGCCTGTAAGAACTCAACACTGGCTAAT
TATTTATACTGGTATGTGATAGTGGAGTGTGAAGATCAGGACACCCAGCAGCGGGACCCAAAGACTCACG
AGATGTACCTGAACGTGATGAGGCGCTTCAGCCAAGCCTTGCTCAAGGGTGACAAGTCTGTGAGAGTCAT
GCGCTCCCTGCTGGCTGCTCAGCAGACCTTTGTAGATCGCCTGGTCCATCTGATGAAGGCAGTGCAGAGA
GAAAGTGGGAACCGCAAGAAGAAGAATGAGAGACTTCAGGCCTTGCTTGGCGATAATGAGAAAATGAACT
TATCAGATGTGGAACTGATCCCATTGCCGCTGGAGCCACAGGTGAAAATAAGGGGCATCATCCCGGAAAC
AGCTACCCTGTTCAAGAGTGCTCTTATGCCTGCCCAGCTGTTCTTCAAGACTGAAGATGGAGGCAAATAC
CCAGTTATTTTCAAGCATGGAGACGACTTGCGTCAAGATCAGCTTATTCTTCAGATCATCTCCCTCATGG
ACAAGCTGTTACGGAAAGAAAACCTGGATTTGAAATTGACCCCATATAAGGTGTTAGCCACTAGCACAAA
ACATGGCTTCATGCAGTTCATCCAGTCGGTTCCTGTCGCAGAAGTTCTTGACACAGAGGGAAGCATTCAG
AACTTTTTCAGGAAATATGCCCCCAGTGAGACTGGACCCTATGGCATCAGTGCAGAGGTCATGGACACTT
ACGTTAAAAGCTGTGCTGGATACTGTGTGATTACGTACATTCTTGGAGTTGGAGACCGGCACCTGGATAA
CCTTTTGTTAACAAAGACAGGCAAACTCTTCCATATAGATTTCGGGTATATTTTGGGTCGAGATCCGAAG
CCCCTCCCTCCTCCGATGAAGCTGAACAAGGAGATGGTGGAAGGGATGGGTGGCACCCAGAGTGAGCAGT
ACCAAGAGTTCCGAAAGCAGTGCTACACAGCCTTCCTCCACCTGCGAAGGTATTCCAATCTGATCTTGAA
CTTGTTCTCCTTGATGGTTGATGCAAACATTCCAGATATTGCTCTTGAACCAGATAAAACTGTAAAAAAG
ATATCCTGCCACAGAAAAATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210937 protein sequence
Red=Cloning site Green=Tags(s)

MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPL
ALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGSAVPVGGTTVSLFGKYGMFRQGM
HDLKVWPNVEADGSEPTRTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKR
SSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSG
PSDHDLKPNATTRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALE
LLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKK
DSQTSASESLSNSGVSSGDIDSSQIITNPLPPVAPPPASKAKEVSDGESLEQDLCTFLISRACKNSTLAN
YLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQR
ESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGKY
PVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQ
NFFRKYAPSETGPYGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPK
PLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKK
ISCHRKM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC024675
ORF Size 2541 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC024675, AAH24675
RefSeq Size 3018 bp
RefSeq ORF 2543 bp
Locus ID 225326
MW 96.8 kDa
Gene Summary Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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