United States
Germany
Japan
United Kingdom
China
Ccar1 (BC079652) Mouse Tagged ORF Clone
CAT＃: MR210925
- TrueORF®
Ccar1 (Myc-DDK-tagged) - Mouse cell division cycle and apoptosis regulator 1 (cDNA clone MGC:90759 IMAGE:6844310)
ORF Plasmid: tGFP
"BC079652" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,176.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610511G16Rik; 9430036H15Rik; Carp1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210925 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTTGGAGGACAGAAGAATCCACCATGGGCTACTCAGTTTACAGCCACTGCGGTCTCACAAC CAGCTGCACTAGGTGTTCAGCAGCCATCACTTCTGGGAGCATCTCCTACCATTTATACCCAGCAGACTGC ATTGGCGGCGGCAGGCCTTACCACACAAACGCCAGCAAACTATCAGTTAACACAAACTGCGGCACTGCAG CAACAAGCTGCAGCTGTATTACAGCAGCAATATTCACAACCTCAGCAGGCCTTGTATAGTGTGCAGCAGC AGTTGCAACAACCTCAGCAGACCATTTTAACACAGCCAGCTGTTGCATTGCCCACAAGCCTTAGCCTGTC GACTCCTCAGCCTGCAGCACAGATTACTGTATCATATCCAACACCAAGGTCCAGTCAACAGCAAACTCAA CCTCAGAAGCAGCGTGTTTTCACAGGAGTGGTTACAAAGCTACATGATACATTTGGATTTGTGGATGAAG ATGTATTCTTTCAGCTTGGTGCTGTTAAAGGGAAAACCCCCCAAGTTGGTGATAGAGTATTGGTTGAAGC AACTTATAATCCTAATATGCCTTTTAAATGGAATGCACAAAGAATTCAAACACTACCAAATCAGAATCAG TCTCAAACGCAACCTTTACTGAAGACTCCGACTGCTGTTATTCAGCCGATTGTGCCACAGACAACGTTTG GTGTTCAGGCACAGCCCCAACCCCAGTCATTATTGCAGGCCCAGATCTCAGCTGCCTCTATTACACCACT ATTGCAGACGCAGCCACAGCCCTTATTACAGCAGCCACAGCAGAAAGCTGGTTTATTGCAGCCTCTTGTC CGAATAGTGTCACAGCCACAACCTGCGCGGAGATTAGATCCACCATCACGATTTTCAGGAAGAAACGACA GAGGGGATCAAGTACCTAATAGAAAAGATGACCGAAGTCGTGAAAGGGACAGAGAAAGACGCAGATCTAG AGAAAGATCACCTCAGAGGAAACGTTCCCGGGAGAGGTCACCCCGGAGAGAAAGAGAGCGCTCCCCTCGG AGAGTCCGTCGTGTCGTTCCACGGTACACAGTGCAGTTTTCAAAGTTTTCTTTAGATTGTCCCAGTTGTG ACATGATGGAACTAAGGCGCCGTTATCAGAACTTATATATTCCTAGTGACTTTTTTGATGCTCAGTTTAC ATGGGTGGATGCTTTCCCTTTGTCAAGACCATTTCAACTGGGAAATTACTGCAATTTTTATGTGATGCAC CGAGAAGTAGAGTCCTTAGAAAAAAATATGGCTGTTCTTGATCCACCTGATGCTGACCACCTGTACAGTG CAAAGGTAATGCTGATGGCTAGCCCTAGTATGGAAGACTTGTATCATAAGTCATGTGCTCTTGCTGAAGA CCCACAAGACCTTCGTGATGGTTTTCAGCATCCTGCTAGACTTGTTAAGTTTCTAGTGGGAATGAAAGGC AAGGATGAAGCCATGGCCATTGGAGGCCACTGGTCTCCTTCGCTGGATGGACCAAACCCAGAAAAAGATC CCTCTGTGTTGATTAAAACTGCCATTCGTTGTTGTAAGGCTCTGACAGGCATTGATCTAAGTGTATGCAC ACAGTGGTACCGTTTTGCAGAGATTCGCTACCATCGCCCTGAGGAGACCCACAAGGGGCGTACAGTTCCA GCTCATGTGGAGACAGTGGTTTTATTTTTCCCGGATGTTTGGCATTGCCTTCCCACCCGCTCAGAGTGGG AAACCCTCTCCCGAGGATACAAGCAGCAGCTGGTCGAGAAGCTTCAGGGTGAACGCAAGAAGGCTGATGG AGAACAGGATGAAGAAGAGAAGGATGATGGTGAAGTTAAAGAGATCGCCACTCCTACCCATTGGTCTAAG CTTGATCCAAAGGCAATGAAGGTAAATGATCTCCGAAAAGAATTAGAAAGTCGAGCTCTCAGTTCCAAAG GACTAAAATCGCAGTTAATAGCTCGCCTAACAAAGCAGCTTAAAATAGAAGAACAAAAAGAAGAGCAGAA GGAATTAGAGAAGTCTGAAAAGGAAGAGGAAGATGAGGATGATAAGAAGTCTGAGGATGATAAAGAGGAA GAAGAAAGAAAACGTCAAGAAGAAGTGGAACGACAGCGTCAAGAAAGAAGATACATTTTGCCTGATGAAC CTGCCATAATTGTGCATCCGAACTGGGCTGCAAAAAGTGGCAAGTTTGATTGCAGCATCATGTCTTTGAG TGTCCTTTTGGATTACAGATTGGAAGATAATAAAGAACATTCTTTTGAGGTTTCACTGTTTGCAGAACTT TTCAATGAAATGCTTCAAAGAGACTTTGGGGTTAGAATATACAAATCATTACTCTCTCTTCCTGAGAAAG AGGACAAAAAAGATAAGGAGAAGAAAAGCAAAAAAGAAGAGAGAAAAGATAAAAAAGAAGAAAGAGAAGA TGATATTGATGAACCAAAACCAAAACGGAGAAAATCAGGCGACGATAAAAAAAAAAAAAAAAAAAGAAGA GAGAAAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210925 protein sequence
Red=Cloning site Green=Tags(s) MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQ QQAAAVLQQQYSQPQQALYSVQQQLQQPQQTILTQPAVALPTSLSLSTPQPAAQITVSYPTPRSSQQQTQ PQKQRVFTGVVTKLHDTFGFVDEDVFFQLGAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQ SQTQPLLKTPTAVIQPIVPQTTFGVQAQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPLV RIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERDRERRRSRERSPQRKRSRERSPRRERERSPR RVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMH REVESLEKNMAVLDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQDLRDGFQHPARLVKFLVGMKG KDEAMAIGGHWSPSLDGPNPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVP AHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKKADGEQDEEEKDDGEVKEIATPTHWSK LDPKAMKVNDLRKELESRALSSKGLKSQLIARLTKQLKIEEQKEEQKELEKSEKEEEDEDDKKSEDDKEE EERKRQEEVERQRQERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAEL FNEMLQRDFGVRIYKSLLSLPEKEDKKDKEKKSKKEERKDKKEEREDDIDEPKPKRRKSGDDKKKKKKRR EKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC079652 |
| ORF Size | 2529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC079652, AAH79652 |
| RefSeq Size | 4477 bp |
| RefSeq ORF | 2531 bp |
| Locus ID | 67500 |
| MW | 97 kDa |
| Gene Summary | Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the retinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (By similarity). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220654 | Ccar1 (untagged) - Mouse cell division cycle and apoptosis regulator 1 (cDNA clone MGC:90759 IMAGE:6844310), (10ug) |
CNY 9,120.00 |
|
| MG210925 | Ccar1 (tGFP-tagged) - Mouse cell division cycle and apoptosis regulator 1 (cDNA clone MGC:90759 IMAGE:6844310) |
CNY 5,320.00 |
|
| MR210925L3 | Lenti ORF clone of Ccar1 (Myc-DDK-tagged) - Mouse cell division cycle and apoptosis regulator 1 (cDNA clone MGC:90759 IMAGE:6844310) |
CNY 6,750.00 |
|
| MR210925L4 | Lenti ORF clone of Ccar1 (mGFP-tagged) - Mouse cell division cycle and apoptosis regulator 1 (cDNA clone MGC:90759 IMAGE:6844310) |
CNY 6,750.00 |
