United States
Germany
Japan
United Kingdom
China
Exo1 (NM_012012) Mouse Tagged ORF Clone
CAT＃: MR210902
- TrueORF®
Exo1 (Myc-DDK-tagged) - Mouse exonuclease 1 (Exo1)
ORF Plasmid: tGFP
"NM_012012" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,208.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730442G03Rik; Msa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210902 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATTCAAGGGTTACTTCAGTTCATCCAAGAAGCTTCTGAACCTGTCAACGTGAAGAAGTACAAAG GACAGGCAGTGGCTGTGGATACCTACTGTTGGCTTCACAAAGGGGCTATTGCTTGTGCTGAAAAGCTAGC CAAAGGGGAACCTACAGATAGGTATGTAGGATTTTGTATGAAGTTTGTTAATATGCTGCTGTCTTATGGG GTCAAGCCGATTCTCATATTTGACGGATGTACTCTACCTTCTAAAAAGGAAGTGGAGCGGTCTCGAAGAG AGAGACGACAAAGCAACCTTCTTAAAGGGAAGCAGCTTCTTCGAGAGGGCAAAGTGTCAGAAGCCCGAGA CTGCTTCGCTCGCTCTATCAACATCACGCACGCCATGGCCCACAAAGTAATAAAAGCTGCTCGGGCCCTA GGAGTGGATTGCCTCGTGGCTCCGTATGAAGCTGATGCTCAGTTGGCCTACCTTAACAAGGCTGGCATCG TGCAGGCTGTCATCACAGAGGACTCTGACCTCCTCGCATTTGGCTGTAAGAAGGTGATTTTAAAAATGGA TCAGTTTGGAAATGGACTGGAAGTGGACCAGGCACGGCTAGGCATGTGCAAGCAGCTTGGGGATGTATTC ACGGAGGAGAAGTTCCGGTACATGTGCATTCTGTCCGGCTGTGACTACCTCGCCTCCCTTCGTGGGATCG GCTTAGCCAAGGCCTGCAAAGTGCTGAGACTGGCCAATAACCCCGATATCGTGAAGGTTATCAAGAAAAT TGGGCATTATCTCAGGATGAATATAACGGTGCCCGAGGATTACATCACAGGATTTATTCGTGCCAACAAT ACTTTCCTCTACCAGCTCGTGTTCGACCCCATCCAAAGGAAGCTGGTCCCTCTGAATGCCTACGGAGATG ACGTTAATCCCGAAACACTGACTTACGCTGGGCAGTACGTCGGTGACTCTGTAGCTCTTCAGATCGCCCT TGGAAATAGAGATGTAAATACTTTTGAACAGATTGATGACTACAGTCCAGACACCATGCCAGCCCACTCA AGAAGCCACAGCTGGAATGAGAAAGCAGGTCAGAAACCACCTGGTACCAACAGCATTTGGCACAAGAATT ATTGTCCTAGACTTGAGGTGAACAGTGTCTCCCACGCTCCTCAACTGAAGGAAAAGCCAAGCACTTTGGG CCTTAAACAAGTGATTAGTACTAAAGGGTTAAATCTTCCCAGGAAGTCTTGTGTGTTGAAAAGACCAAGA AATGAAGCGCTGGCTGAAGATGACCTGTTGAGCCAGTATTCGTCAGTTTCAAAGAAGATCAAGGAAAATG GCTGTGGGGATGGCACATCACCTAACTCTTCTAAAATGTCCAAGTCCTGCCCCGATTCTGGGACTGCTCA CAAGACAGATGCACACACCCCGTCTAAGATGAGGAATAAATTTGCAACGTTCTTACAGAGGAGGAATGAA GAAAGCGGTGCAGTCGTGGTTCCAGGGACCAGAAGCAGGTTTTTTTGCAGTTCTCAGGATTTTGACAATT TCATACCAAAAAAAGAAAGCGGCCAGCCTCTAAATGAAACTGTGGCCACTGGCAAAGCCACCACCAGCCT CCTCGGGGCACTGGACTGTCCAGACACGGAAGGCCACAAGCCGGTTGATGCAAATGGGACGCACAATCTG AGCTCTCAGATTCCAGGCAATGCAGCTGTGTCTCCTGAGGATGAGGCTCAGTCCTCTGAGACCAGCAAAC TCTTGGGGGCCATGTCCCCACCCAGTCTGGGGACACTAAGAAGCTGTTTCAGTTGGTCTGGCACTCTCAG GGAATTTTCTCGAACTCCAAGTCCCTCAGCAAGCACTACATTGCAGCAGTTCCGGAGAAAGAGCGACCCC CCTGCCTGTCTTCCTGAGGCCTCTGCCGTGGTCACTGACAGGTGCGACTCAAAGAGCGAGATGCTGGGTG AGACGTCTCAGCCCTTGCACGAGCTGGGCTGCTCCTCGCGGTCTCAGGAAAGCATGGACTCCTCGTGCGG CCTAAACACGTCGAGCCTGTCCCAGCCTTCCAGTAGGGATTCAGGTTCAGAGGAGTCGGATTGTAACAAT AAGTCACTTGATAATCAAGGGGAACAGAACTCCAAGCAACACTTACCTCACTTTTCAAAGAAAGACGGAC TTAGACGGAACAAGGTTCCTGGGCTCTGTAGGTCTAGTTCTATGGACTCTTTTTCTACAACCAAGATCAA GCCCCTGGTGCCTGCCAGGGTCAGTGGCCTGAGCAAGAAGTCAGGAAGCATGCAGACGAGAAAACACCAT GATGTGGAGAACAAGCCAGGCCTACAGACGAAGATCAGCGAGCTCTGGAAAAACTTTGGGTTTAAAAAAG ATTCTGAAAAGCTTCCTTCTTGTAAGAAGCCCCTGTCTCCAGTCAAGGACAATATCCAACTCACTCCAGA GACAGAAGATGAGATCTTTAACAAGCCCGAGTGTGTACGCGCTCAGAGAGCAATATTTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210902 protein sequence
Red=Cloning site Green=Tags(s) MGIQGLLQFIQEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYG VKPILIFDGCTLPSKKEVERSRRERRQSNLLKGKQLLREGKVSEARDCFARSINITHAMAHKVIKAARAL GVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVILKMDQFGNGLEVDQARLGMCKQLGDVF TEEKFRYMCILSGCDYLASLRGIGLAKACKVLRLANNPDIVKVIKKIGHYLRMNITVPEDYITGFIRANN TFLYQLVFDPIQRKLVPLNAYGDDVNPETLTYAGQYVGDSVALQIALGNRDVNTFEQIDDYSPDTMPAHS RSHSWNEKAGQKPPGTNSIWHKNYCPRLEVNSVSHAPQLKEKPSTLGLKQVISTKGLNLPRKSCVLKRPR NEALAEDDLLSQYSSVSKKIKENGCGDGTSPNSSKMSKSCPDSGTAHKTDAHTPSKMRNKFATFLQRRNE ESGAVVVPGTRSRFFCSSQDFDNFIPKKESGQPLNETVATGKATTSLLGALDCPDTEGHKPVDANGTHNL SSQIPGNAAVSPEDEAQSSETSKLLGAMSPPSLGTLRSCFSWSGTLREFSRTPSPSASTTLQQFRRKSDP PACLPEASAVVTDRCDSKSEMLGETSQPLHELGCSSRSQESMDSSCGLNTSSLSQPSSRDSGSEESDCNN KSLDNQGEQNSKQHLPHFSKKDGLRRNKVPGLCRSSSMDSFSTTKIKPLVPARVSGLSKKSGSMQTRKHH DVENKPGLQTKISELWKNFGFKKDSEKLPSCKKPLSPVKDNIQLTPETEDEIFNKPECVRAQRAIFH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012012 |
| ORF Size | 2514 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012012.4 |
| RefSeq Size | 5506 bp |
| RefSeq ORF | 2514 bp |
| Locus ID | 26909 |
| UniProt ID | Q9QZ11 |
| MW | 92 kDa |
| Gene Summary | 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200610 | Exo1 (untagged) - Mouse exonuclease 1 (Exo1), (10ug) |
CNY 9,208.00 |
|
| MG210902 | Exo1 (tGFP-tagged) - Mouse exonuclease 1 (Exo1) |
CNY 10,808.00 |
|
| MR210902L3 | Lenti ORF clone of Exo1 (Myc-DDK-tagged) - Mouse exonuclease 1 (Exo1) |
CNY 10,260.00 |
|
| MR210902L4 | Lenti ORF clone of Exo1 (mGFP-tagged) - Mouse exonuclease 1 (Exo1) |
CNY 10,260.00 |
