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Sidt2 (NM_172257) Mouse Tagged ORF Clone
CAT＃: MR210871
- TrueORF®
Sidt2 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2)
ORF Plasmid: tGFP
"NM_172257" in other vectors (4)
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CNY 6,096.00
CNY 7,320.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B930096O19; BC023957; CGI-40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210871 representing NM_172257
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCCTGGCGTCTGCCCTTGTGCGTGCTCTTGGTGGCCTCCGTCGAGAGCCACCTGGGGGCCCTGG GGCCCAAGAACGTCTCGCAGAAAGACGCGGAGTTTGAGCGCACCTACGCGGACGACGTCAACAGCGAGCT GGTCAACATCTACACCTTCAACCACACCGTGACCCGCAACCGGACCGAGGGTGTGCGAGTGTCTGTGAAT GTCCTGAACAAGCAGAAAGGGGCGCCTTTGCTGTTTGTGGTCCGCCAGAAGGAGGCTGTTGTGTCCTTCC AGGTGCCCCTAATCCTTCGAGGACTATATCAGCGGAAGTACCTCTACCAAAAAGTGGAACGAACTCTGTG TCAGCCCCCCACCAAGAATGAGTCTGAGATCCAGTTTTTCTATGTGGACGTGTCTACCCTGTCACCCGTC AATACCACTTACCAGCTCCGAGTCAACCGTGTGGACAATTTTGTGCTCAGGACTGGAGAGCTGTTTACTT TTAATACCACTGCAGCCCAGCCCCAGTACTTCAAATACGAGTTTCCTGATGGTGTGGACTCGGTAATTGT CAAGGTGACCTCCAAGAAGGCCTTCCCCTGCTCAGTCATCTCCATCCAAGATGTCCTGTGCCCTGTCTAT GATCTGGACAACAATGTAGCCTTCATTGGCATGTACCAGACGATGACTAAGAAGGCAGCCATCACTGTGC AGCGGAAAGACTTCCCCAGCAACAGCTTCTATGTGGTGGTGGTAGTGAAGACTGAGGACCAGGCCTGCGG AGGGTCCTTGCCCTTCTACCCTTTTGTGGAAGATGAGCCAGTGGATCAAGGGCACCGTCAGAAAACACTG TCAGTGCTGGTCTCTCAGGCTGTCACATCTGAGGCCTATGTTGGTGGGATGCTCTTTTGCCTGGGCATAT TCTTGTCCTTCTACCTGCTGACTGTGCTGCTGGCCTGTTGGGAGAACTGGAGGCAAAGGAAGAAGACCTT GCTGTTGGCCATAGACCGAGCCTGCCCAGAAAGTGGTCACGCCCGGGTCTTGGCTGATTCATTTCCTGGC AGTGCCCCTTACGAGGGTTACAACTATGGCTCCTTTGAAAATGGTTCCGGATCCACTGACGGGTTGGTTG AAAGCGCAGGTTCAGGGGACCTCTCCTACAGTTACCAGGACCGCTCCTTTGACGCAGTGGGTCCTCGGCC TCGACTGGACTCCATGAGCTCCGTGGAAGAGGATGACTACGACACACTGACTGACATCGACTCAGACAAA AACGTCATTCGAACCAAGCAATACCTCTGTGTGGCTGATCTGGCACGAAAGGACAAACGTGTTTTGCGGA AAAAGTACCAGATTTACTTCTGGAACATAGCCACCATTGCGGTCTTCTACGCACTTCCTGTGGTGCAGCT GGTGATCACCTACCAGACGGTGGTGAATGTCACAGGGAACCAGGACATCTGCTACTACAACTTCCTCTGT GCCCACCCGCTGGGCAACCTCAGCGCCTTCAACAACATCCTCAGCAACTTGGGGTACATCCTGCTGGGGC TGCTCTTCCTGCTCATCATCCTGCAGCGAGAGATCAATCATAACCGGGCCCTGCTGCGGAATGACCTCTA TGCTCTGGAGTGTGGGATCCCCAAACACTTTGGTCTGTTTTACGCCATGGGCACAGCACTGATGATGGAG GGGCTACTTAGTGCCTGTTACCACGTCTGCCCCAACTACACCAACTTCCAGTTTGATACCTCCTTCATGT ACATGATTGCTGGCCTCTGCATGCTGAAGCTCTACCAGAAGCGGCACCCAGATATCAACGCCAGTGCCTA CAGTGCATATGCCTGCTTGGCCATCGTCATCTTCTTCTCCGTTCTGGGCGTGGTGTTTGGCAAAGGGAAC ACGGCCTTCTGGATTGTCTTCTCCGTCATTCACATCATCTCCACCCTGCTCCTCAGCACTCAGCTCTATT ACATGGGCCGCTGGAAGCTGGACTCCGGGATCTTCCGCCGCATCCTCCATGTGCTCTACACAGACTGCAT CCGGCAGTGCAGCGGGCCCCTTTACACGGACCGCATGGTGCTTCTGGTCATGGGCAACATTATCAACTGG TCGCTGGCTGCATACGGACTCATCATGCGCCCCAATGACTTTGCTTCCTACTTGCTGGCAATTGGCATCT GCAACCTGCTGCTTTATTTCGCCTTCTACATCATCATGAAGCTCCGGAGCGGCGAGAGGATCAAGCTCAT CCCTCTGCTCTGCATCGTCTGCACCTCCGTGGTCTGGGGCTTCGCGCTCTTCTTCTTCTTCCAGGGACTG AGCACGTGGCAGAAAACCCCCGCAGAGTCCAGGGAGCACAACCGCGACTGCATCCTCCTCGACTTCTTTG ATGACCACGATATCTGGCACTTCCTGTCCTCCATTGCCATGTTTGGGTCCTTCCTGGTTTTGCTGACGTT GGATGACGACTTGGACACAGTACAGCGGGACAAGATCTATGTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210871 representing NM_172257
Red=Cloning site Green=Tags(s) MIAWRLPLCVLLVASVESHLGALGPKNVSQKDAEFERTYADDVNSELVNIYTFNHTVTRNRTEGVRVSVN VLNKQKGAPLLFVVRQKEAVVSFQVPLILRGLYQRKYLYQKVERTLCQPPTKNESEIQFFYVDVSTLSPV NTTYQLRVNRVDNFVLRTGELFTFNTTAAQPQYFKYEFPDGVDSVIVKVTSKKAFPCSVISIQDVLCPVY DLDNNVAFIGMYQTMTKKAAITVQRKDFPSNSFYVVVVVKTEDQACGGSLPFYPFVEDEPVDQGHRQKTL SVLVSQAVTSEAYVGGMLFCLGIFLSFYLLTVLLACWENWRQRKKTLLLAIDRACPESGHARVLADSFPG SAPYEGYNYGSFENGSGSTDGLVESAGSGDLSYSYQDRSFDAVGPRPRLDSMSSVEEDDYDTLTDIDSDK NVIRTKQYLCVADLARKDKRVLRKKYQIYFWNIATIAVFYALPVVQLVITYQTVVNVTGNQDICYYNFLC AHPLGNLSAFNNILSNLGYILLGLLFLLIILQREINHNRALLRNDLYALECGIPKHFGLFYAMGTALMME GLLSACYHVCPNYTNFQFDTSFMYMIAGLCMLKLYQKRHPDINASAYSAYACLAIVIFFSVLGVVFGKGN TAFWIVFSVIHIISTLLLSTQLYYMGRWKLDSGIFRRILHVLYTDCIRQCSGPLYTDRMVLLVMGNIINW SLAAYGLIMRPNDFASYLLAIGICNLLLYFAFYIIMKLRSGERIKLIPLLCIVCTSVVWGFALFFFFQGL STWQKTPAESREHNRDCILLDFFDDHDIWHFLSSIAMFGSFLVLLTLDDDLDTVQRDKIYVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172257 |
| ORF Size | 2496 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172257.4, NP_758461.1 |
| RefSeq Size | 4221 bp |
| RefSeq ORF | 2499 bp |
| Locus ID | 214597 |
| UniProt ID | Q8CIF6 |
| MW | 95 kDa |
| Gene Summary | Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA and DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251, PubMed:27846365, PubMed:28724756). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA (PubMed:27846365). Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (PubMed:26067272).[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Systemic RNA Interference Deficiency-1 (SID-1) extracellular domain selectively binds long doublestrandedRNA and is required for RNA transport by SID-1
,Li, W;Koutmou, KS;Leahy, DJ;Li, M;,
JBC
,PubMed ID 26067272
[SIDT2]
|
|
Systemic RNA Interference Deficiency-1 (SID-1) extracellular domain selectively binds long double-stranded RNA and is required for RNA transport by SID-1
,Li, W;Koutmou, KS;Leahy, DJ;Li, M;,
J. Biol. Chem.
[SIDT2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221923 | Sidt2 (untagged) - Mouse SID1 transmembrane family, member 2 (Sidt2), (10ug) |
CNY 10,640.00 |
|
| MG210871 | Sidt2 (tGFP-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2) |
CNY 7,696.00 |
|
| MR210871L3 | Lenti ORF clone of Sidt2 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2) |
CNY 9,220.00 |
|
| MR210871L4 | Lenti ORF clone of Sidt2 (mGFP-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2) |
CNY 9,220.00 |
