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Kdm1b (NM_172262) Mouse Tagged ORF Clone
CAT＃: MR210840
- TrueORF®
Kdm1b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 1B (Kdm1b)
ORF Plasmid: tGFP
"NM_172262" in other vectors (4)
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CNY 6,056.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4632428N09Rik; AI482520; Aof1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210840 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCATCTCGAGGGAGGTCAAAGAAGAGAAGCAATTTGGAGCTTTCTCCTGATAACCTGCCTTTGA GGAGCTCTGGGCGGCAGGCAAAGAAGAAAGCAGTAGAGATCCCGGATGAGGATGAAGATGGTTCGTCGGA GAAGAAGTACCGGAAATGTGAAAAGGCAGGCTGTACGGCGGCGTACCCTGTGTGCTTTGCAAGCGCTTCT GAAAGATGTGCCAAAAATGGTTACACCTCCCGATGGTATCACCTTTCCTGTGGGGAACACTTCTGCAATG AATGCTTTGACCACTACTACAGAAGCCACAAAGATGGGTATGACAAATACAGTGCATGGAAAAGAGTGTG GACCAGCAACGGCAAGACGGAGCCAAGCCCCAAGGCGTTCATGGCAGACCAGCAGCTGCCCTACTGGGTG CAGTGCACAAAACCCGAGTGTGGAAAGTGGAGGCAGCTGACCAAGGAGATCCAGCTTACTCCACACATGG CGAGGACTTACCGCTGTGGCATGAAGCCAAATACTATTACCAAGCCTGACACCCCCGATCATTGTTCTTT CCCCGAGGATCTGAGAGTGCTGGAAGTTTCCAACCACTGGTGGTACCCCATGCTCATCCAGCCTCCGTTG CTGAAGGACAGTGTGGCAGCACCGCTGCTTTCTGCCTACTACCCCGACTGCGTTGGCATGAGCCCCTCCT GCACCAGCACACATCGTGCCACCGTCACAGCTGCTACCACAACCACCGGCAGTGCCAGCCCGGGGGAGAT GGAGCCCTCCAAGGCAGCCCCCTCTTCACTTGTCCTAGGCATGAACCGGTACTTCCAGCCGTTCTACCAG CCCAACGAGTGTGGGAAAGCGCTGTGCGTGAGGCCAGACGTGATGGAGCTGGATGAGCTCTACGAGTTCC CAGAGTATTCGCGGGACCCCACCATGTACCTGGCTTTGAGAAACCTCATCCTCGCACTGTGGTACACAAA CTGCAAAGAAGCTCTCACCCCTCAGAAGTGCATTCCCCACATCATTGTCCGGGGCCTTGTCCGCATCAGA TGCGTTCAGGAAGTGGAGAGGATTCTTTACTTCATGACGAGGAAAGGCCTCATCAACACAGGCGTTCTCA CGGTGGCAGCCGGCCAGCATCTTCTTCCTAAACACTACCACAATAAATCTGTTCTGGTTGTTGGGGCTGG TCCAGCAGGCTTAGCAGCTGCTAGGCAACTGCATAACTTTGGGATGAAGGTGACTGTCCTGGAAGCCAAA GACCGGATTGGAGGCCGAGTATGGGATGACAAGTCCTTTAAAGGCGTGGTAGTAGGAAGAGGACCCCAGA TTGTCAATGGCTGTATTAATAACCCAGTAGCACTAATGTGTGAGCAACTTGGCATCAGCATGCGTAAGTT GGGAGAGAGATGTGACTTAATTCAGGAAGGTGGACGGATAACTGACCCCACTGTTGACAAACGCATGGAC TTTCATTTTAATGCTCTCTTGGATGTTGTCTCTGAGTGGAGGAAGGATAAGACTCTGCTCCAAGATGTCC CTTTAGGAGAGAAGATAGAGGAGATCTACCGAGCTTTTGTGAAGGAGTCTGGCATCCAGTTCAGCGAGCT GGAGGGACAGGTGCTGCAGTTCCACCTGAGTAACCTGGAGTATGCTTGTGGCAGCAGCCTGCACCAGGTG TCTGCTCGCTCCTGGGATCACAATGAATTCTTTGCCCAGTTTGCTGGTGACCATACCCTCCTCACACCAG GCTACTCCACTATCATTGAGAAACTGGCTGAAGGACTAGACATTCGACTCAAATCTCCAGTGCAAAGTAT AGATTATACTGGAGATGAAGTGCAGGTTACCACGACAGATGGTATGGGGCATTCTGCACAAAAGGTGTTA GTCACTGTGCCATTGGCCATACTACAGAGAGGTGCCATCCAGTTTAATCCACCATTGTCAGAAAAGAAGA TGAAAGCCATCAACAGCTTGGGTGCAGGCATCATTGAAAAGATTGCCTTGCAGTTTCCATATAGATTTTG GGACAGTAAAGTTCAAGGAGCTGACTTTTTTGGCCATGTTCCTCCCAGTGCCAGCCAACGAGGCCTCTTC GCTGTATTCTATGACATGGACTCTCAGCAGAGTGTGCTGATGTCGGTGATCACAGGGGAGGCAGTGGCGT CCCTCAGGACCATGGATGACAAGCAGGTGCTGCAGCAGTGCATGGGCATCCTTCGGGAGCTGTTCAAGGA GCAGGAGATCCCAGAACCCACAAAGTATTTTGTCACACGGTGGAGCACAGAGCCATGGATCCAGATGGCA TATAGTTTCGTGAAGACCTTTGGAAGTGGCGAGGCCTATGATATTATTGCTGAAGAAATACAAGGAACTG TCTTTTTTGCTGGTGAGGCAACAAACAGGCATTTCCCACAAACTGTTACTGGGGCATATTTAAGTGGTGT ACGAGAAGCAAGCAAGATTGCAGCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210840 protein sequence
Red=Cloning site Green=Tags(s) MAASRGRSKKRSNLELSPDNLPLRSSGRQAKKKAVEIPDEDEDGSSEKKYRKCEKAGCTAAYPVCFASAS ERCAKNGYTSRWYHLSCGEHFCNECFDHYYRSHKDGYDKYSAWKRVWTSNGKTEPSPKAFMADQQLPYWV QCTKPECGKWRQLTKEIQLTPHMARTYRCGMKPNTITKPDTPDHCSFPEDLRVLEVSNHWWYPMLIQPPL LKDSVAAPLLSAYYPDCVGMSPSCTSTHRATVTAATTTTGSASPGEMEPSKAAPSSLVLGMNRYFQPFYQ PNECGKALCVRPDVMELDELYEFPEYSRDPTMYLALRNLILALWYTNCKEALTPQKCIPHIIVRGLVRIR CVQEVERILYFMTRKGLINTGVLTVAAGQHLLPKHYHNKSVLVVGAGPAGLAAARQLHNFGMKVTVLEAK DRIGGRVWDDKSFKGVVVGRGPQIVNGCINNPVALMCEQLGISMRKLGERCDLIQEGGRITDPTVDKRMD FHFNALLDVVSEWRKDKTLLQDVPLGEKIEEIYRAFVKESGIQFSELEGQVLQFHLSNLEYACGSSLHQV SARSWDHNEFFAQFAGDHTLLTPGYSTIIEKLAEGLDIRLKSPVQSIDYTGDEVQVTTTDGMGHSAQKVL VTVPLAILQRGAIQFNPPLSEKKMKAINSLGAGIIEKIALQFPYRFWDSKVQGADFFGHVPPSASQRGLF AVFYDMDSQQSVLMSVITGEAVASLRTMDDKQVLQQCMGILRELFKEQEIPEPTKYFVTRWSTEPWIQMA YSFVKTFGSGEAYDIIAEEIQGTVFFAGEATNRHFPQTVTGAYLSGVREASKIAAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172262 |
| ORF Size | 2481 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172262.3, NP_758466.1 |
| RefSeq Size | 4987 bp |
| RefSeq ORF | 2481 bp |
| Locus ID | 218214 |
| UniProt ID | Q8CIG3 |
| MW | 92.6 kDa |
| Gene Summary | Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221893 | Kdm1b (untagged) - Mouse lysine (K)-specific demethylase 1B (Kdm1b), (10ug) |
CNY 10,550.00 |
|
| MG210840 | Kdm1b (tGFP-tagged) - Mouse amine oxidase, flavin containing 1 (Aof1) |
CNY 6,370.00 |
|
| MR210840L3 | Lenti ORF clone of Kdm1b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 1B (Kdm1b) |
CNY 7,700.00 |
|
| MR210840L4 | Lenti ORF clone of Kdm1b (mGFP-tagged) - Mouse lysine (K)-specific demethylase 1B (Kdm1b) |
CNY 7,700.00 |
