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Epha5 (BC057401) Mouse Tagged ORF Clone
CAT＃: MR210806
- TrueORF®
Epha5 (Myc-DDK-tagged) - Mouse Eph receptor A5 (cDNA clone MGC:62343 IMAGE:5697758)
ORF Plasmid: tGFP
"BC057401" in other vectors (4)
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CNY 6,000.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ehk1, Hek7, Rek7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210806 representing BC057401
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCTCCGGGCCCCGCGGTGCGGGACACCGACGGACCCAGGGCAGAGGTGGCGGCGACGACACCC CCCGCGTCCCTGCCTCTCTGGCAGGCTGCTATTCGGCACCTCTAAAGGGCCCCCTCTGGACGTGCCTTCT CTTGTGTGCGGCGCTCCGGACCCTTTTGGCCAGCCCCAGCAACGAAGTGAATTTGTTGGATTCGCGCACT GTCATGGGGGACCTTGGATGGATTGCTTTTCCAAAGAACGGGTGGGAAGAGATTGGTGAAGTTGATGAGA ACTATGCCCCCATCCACACATACCAAGTGTGCAAAGTTATGGAACAGAATCAGAATAATTGGCTGTTGAC CAGTTGGATCTCTAACGAAGGTGCTTCCAGAATCTTTATTGAACTCAAGTTTACTTTAAGGGACTGCAAC AGCCTTCCTGGAGGACTGGGGACTTGTAAGGAGACATTTAACATGTATTATTTTGAATCAGATGATGAGA ATGGGAGAAGTATCAAAGAGAACCAATACATCAAGATTGATACCATCGCTGCAGATGAGAGCTTCACAGA ACTTGATCTTGGTGACCGTGTCATGAAACTGAATACAGAGGTCAGAGATGTCGGACCTCTGAGCAAAAAG GGATTTTATCTTGCTTTCCAAGATGTCGGTGCTTGCATTGCTCTGGTTTCTGTCCGTGTCTACTATAAAA AGTGTCCCTCTGTAGTAAGACACTTGGCTATCTTCCCTGACACTATCACTGGAGCAGATTCATCACAGTT GTTAGAGGTGTCAGGCTCCTGCGTCAACCATTCTGTGACAGATGATCCTCCCAAGATGCATTGCAGTGCT GAAGGGGAGTGGCTGGTTCCCATTGGGAAATGCATGTGCAAGGCTGGATATGAAGAGAAAAATGGTACCT GCCAAGCTCCTTCTCCAGTCACCAATGTGAAAAAGGGGAAGATTGCAAAGAACAGCATTTCTTTGTCTTG GCAAGAGCCAGATCGCCCCAATGGAATTATCCTGGAGTATGAAATCAAGTACTTTGAAAAGGACCAAGAG ACCAGTTACACAATTATCAAGTCTAAAGAGACCAGTATTACAGCCGAGGGCCTGAAACCTGCATCTGTGT ATGTCTTCCAAATTCGAGCACGTACAGCAGCAGGCTACGGCGTCTTCAGTCGAAGATTTGAGTTTGAAAC CACACCAGTGTTTGCAGCATCTAATGATCAAAGCCAGATTCCCATCATTGCAGTGTCAGTGACAGTGGGA GTCATCTTGTTGGCAGTGATGATCGGCTTCCTCCTCAGTGGCAGGCGGTGTGGCTACAGCAAAGCAAAGC AGGATCCAGAAGAGGAAAAGATGCACTTTCATAACGGGCACATTAAACTGCCAGGAGTCAGAACCTATAT TGATCCGCACACTTATGAAGATCCCAATCAAGCTGTTCATGAATTTGCGAAGGAGATTGAAGCTTCATGC ATCACCATTGAGAGAGTGATCGGAGCAGGTGAATTTGGTGAAGTTTGCAGTGGACGTTTGAAACTACCTG GAAAAAGAGAATTACCTGTGGCTATCAAAACTCTTAAAGTAGGCTATACTGAAAAGCAGCGCAGAGATTT CCTGGGTGAAGCAAGTATTATGGGGCAGTTCGATCATCCAAACATCATCCATCTAGAAGGTGTTGTGACT AAAAGCAAACCTGTGATGATAGTGACAGAGTACATGGAGAACGGCTCCTTAGACACGTTTTTAAAGAAAA ACGATGGGCAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGCATCGCTGCAGGAATGAAGTACCT TTCTGACATGGGCTACGTGCATAGAGACCTTGCTGCTAGAAACATCTTAATCAACAGTAACCTTGTGTGC AAGGTGTCTGACTTTGGACTTTCCAGGGTACTGGAAGATGATCCTGAGGCAGCCTACACCACAAGGGGAG GCAAAATTCCAATCAGATGGACTGCCCCGGAGGCAATAGCTTTTCGAAAGTTCACCTCTTCCAGTGATGT CTGGAGCTATGGCATTGTAATGTGGGAAGTTGTATCTTATGGAGAGAGACCCTACTGGGAGATGACCAAT CAGGATGTGATCAAGGCAGTAGAAGAAGGCTACCGCCTGCCAAGCCCCATGGATTGCCCTGCTGCTCTCT ATCAATTAATGCTGGATTGCTGGCAGAAAGATCGAAACAGCAGGCCCAAGTTTGATGAAATCGTCAACAT GCTGGACAAACTGATACGAAACCCAAGTAGTCTGAAGACACTGGTGAATGCGTCGAGCAGAGTGTCTACA TTGTTGGCAGAACATGGTTCTTTGGGGTCTGGGGCCTACAGATCAGTAGGTGAATGGCTGGAAGCAATCA AAATGGGTCGGTACACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGTGGCTCAGGTGAC CTTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210806 representing BC057401
Red=Cloning site Green=Tags(s) MRGSGPRGAGHRRTQGRGGGDDTPRVPASLAGCYSAPLKGPLWTCLLLCAALRTLLASPSNEVNLLDSRT VMGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCN SLPGGLGTCKETFNMYYFESDDENGRSIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKK GFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAIFPDTITGADSSQLLEVSGSCVNHSVTDDPPKMHCSA EGEWLVPIGKCMCKAGYEEKNGTCQAPSPVTNVKKGKIAKNSISLSWQEPDRPNGIILEYEIKYFEKDQE TSYTIIKSKETSITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETTPVFAASNDQSQIPIIAVSVTVG VILLAVMIGFLLSGRRCGYSKAKQDPEEEKMHFHNGHIKLPGVRTYIDPHTYEDPNQAVHEFAKEIEASC ITIERVIGAGEFGEVCSGRLKLPGKRELPVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIHLEGVVT KSKPVMIVTEYMENGSLDTFLKKNDGQFTVIQLVGMLRGIAAGMKYLSDMGYVHRDLAARNILINSNLVC KVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAFRKFTSSSDVWSYGIVMWEVVSYGERPYWEMTN QDVIKAVEEGYRLPSPMDCPAALYQLMLDCWQKDRNSRPKFDEIVNMLDKLIRNPSSLKTLVNASSRVST LLAEHGSLGSGAYRSVGEWLEAIKMGRYTEIFMENGYSSMDAVAQVTLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057401 |
| ORF Size | 2457 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057401.1 |
| RefSeq Size | 4018 bp |
| RefSeq ORF | 2459 bp |
| Locus ID | 13839 |
| MW | 147.3 kDa |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220716 | Epha5 (untagged) - Mouse Eph receptor A5 (cDNA clone MGC:62343 IMAGE:5697758), (10ug) |
CNY 9,120.00 |
|
| MG210806 | Epha5 (tGFP-tagged) - Mouse Eph receptor A5 (cDNA clone MGC:62343 IMAGE:5697758) |
CNY 5,130.00 |
|
| MR210806L3 | Lenti ORF clone of Epha5 (Myc-DDK-tagged) - Mouse Eph receptor A5 (cDNA clone MGC:62343 IMAGE:5697758) |
CNY 6,560.00 |
|
| MR210806L4 | Lenti ORF clone of Epha5 (mGFP-tagged) - Mouse Eph receptor A5 (cDNA clone MGC:62343 IMAGE:5697758) |
CNY 6,560.00 |
