Suv420h1 (BC075709) Mouse Tagged ORF Clone

CAT#: MR210794

  • TrueORF®

Suv420h1 (Myc-DDK-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (cDNA clone MGC:78059 IMAGE:3661032)

ORF Plasmid: DDK tGFP



  "BC075709" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,170.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-Suv420h1
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC18702, Suv4-20h1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210794 representing BC075709
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTGGTTGGGAGACTCCAAGAACATGGTGGTGAATGGCAGGAGAAATGGAGGCAAGTTGTCTAATG
ACCATCAGCAGAATCAATCAAAATTACAGCAGCACTCGGGCAAGGACACCCTGAAGACCGGCAGAAACGC
CGTTGAGAGGCGGTCCAACAGATGTCATGGTAACTCGGGATTTGAAGGGCAGAGCCGCTATGTGCCGTCC
TCTGGAATGTCCGCCAAGGAGCTCTGTGAGAACGATGACTTAGCAACCAGTTTGGTTCTTGATCCCTACT
TAGGTTTTCAGACACACAAAATGAACACTAGATTTCGGCCTATAAAAGGAAGGCAAGAAGAGCTAAAGGA
AGTAATTGAACGCTTTAAGAAAGATGAACACTTAGAGAAAGCTTTCAAATGTTTGACTTCTGGGGAATGG
GCACGGCATTATTTTCTCAACAAAAACAAAATGCAGGAGAAATTATTCAAGGAACATGTCTTTATTTACT
TGCGGATGTTTGCAACTGACAGTGGATTTGAAATACTGCCTTGTAATAGATATTCTTCAGAACAAAATGG
AGCCAAGATAGTTGCAACAAAAGAGTGGAAACGAAATGACAAAATAGAATTACTGGTGGGTTGTATTGCC
GAACTTTCAGAAATTGAGGAGAACATGCTACTTAGACACGGAGAAAACGACTTCAGTGTCATGTATTCCA
CAAGGAAAAATTGTGCTCAACTCTGGCTCGGTCCTGCTGCATTTATAAATCATGATTGCAGACCTAACTG
TAAGTTTGTGTCAACTGGTCGAGATACAGCATGTGTTAAGGCTCTGAGAGATATTGAACCTGGAGAAGAA
ATTTCTTGTTACTATGGAGATGGCTTTTTTGGAGAAAATAATGAGTTCTGCGAATGTTATACTTGTGAAA
GACGGGGAACTGGTGCTTTTAAATCACGAGTAGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATA
CGGACTTAGAGAAACAGATAAACGCTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAACTCAGAC
AGTCAGTCTGTCAGCTCTAACACAGATGCAGACACCACTCAGGAAAAAGACAATGCAACTTCTAATCGAA
AATCTTCAGTTGGTGTGAAAAAGAGCAGCAAGAGTCGAGCTCTGACGAGGCCGTCCATGCCGAGAGTCCC
GGCTGCTTCCAACTCTACCTCAGCCAAGCTAGTGCACACCAACAATCCCCGGGTACCAAAGAAACTGAGA
AAGCCGGCAAAGCCTTTACTCTCCAAGATCAGACTGCGGAATCACTGCAAGCGGCTGGACCAGAAGAGCG
CATCCCGCAAGCTCGAGATGGGGAGCTTAGTGCTTAAGGAGCCCAAAGTTGTGCTATATAAAAATTTGCC
AATTAAGAAAGAAAGGGAGCCAGAGGGACCAGCCCATGCTGCAGTGGGGAGTGGGTGCTTGACTAGACAT
GCTGCGAGAGAACACAGGCAGAGTCATGGGAGAGGTGCTCATTCGCAGGGCGACAGTTTGCCCTGCACCT
ACACAACCCGGCGCTCTTTGAGGACAAGGACAGGTCTGAAGGAGACCACTGACATCAAGCTTGAACCAAG
TCCCTTGGATGGCTATAAAAATGGTATACTGGAACCTTGCCCAGACAGTGGCCAGCAGCCAACCCCAGAG
GTGCTGGAAGAACTGGCTCCTGAGACTGCACACAGGGAGGAAGCATCCCAGGAGTGTCCCAAGAACGACT
CCTGCCTGTCACGAAAGAAATTTCGACAAGTGAAACCTGTGAAACACTTAGCAAAGACAGAGGACTGCAG
TCCAGAGCACAGCTTCCCTGGGAAAGACGGGCTGCCAGATTTGCCAGGGTCTCATCCTGATCAAGGTGAG
CCCAGTGGCACAGTCAGGGTGCCCGTGAGCTACACGGACTCTGCTCCCTCACCGGTTGGCTGCTCTGTTG
TCACACCCGACAGCTTCACAAAAGACAGCTTCAGAACTGCACAAAGTAAAAAGAAGCGGCGGGTCACCAG
GTACGATGCACAGCTGATCCTGGAGAACAGCTCTGGAATCCCCAAGCTGACGCTTCGCAGGCGGCACGAC
AGCAGCAGCAAGACAAACGACCATGAGAGTGACGGCGTGAACTCCTCCAAGATCAGCATCAAACTCAGCA
AGGACCACGACAGTGACAGCAACCTCTATGTTGCCAAGCTCAGTAACGGGGTCAGCGCGGGGCCGGGCAG
CAGCTCCACCAAGCTCAAGATCCAGCTCAAGCGGGATGAGGAGAGCAGGGGGCCATGTGCAGAGGGCCTG
CACGAGAACGGGGTGTGCTGCAGCGACCCCCTCTCCCTGCTCGAGTCCCAGATGGAGGTGGACGACTACA
GTCAATATGAGGAGGACAGCACAGATGAATCCTCATCTTCCTCCGGCAAAGCGGCTGAGGCTAATTGTTG
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210794 representing BC075709
Red=Cloning site Green=Tags(s)

MKWLGDSKNMVVNGRRNGGKLSNDHQQNQSKLQQHSGKDTLKTGRNAVERRSNRCHGNSGFEGQSRYVPS
SGMSAKELCENDDLATSLVLDPYLGFQTHKMNTRFRPIKGRQEELKEVIERFKKDEHLEKAFKCLTSGEW
ARHYFLNKNKMQEKLFKEHVFIYLRMFATDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIA
ELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEE
ISCYYGDGFFGENNEFCECYTCERRGTGAFKSRVGLPAPAPVINSKYGLRETDKRLNRLKKLGDSSKNSD
SQSVSSNTDADTTQEKDNATSNRKSSVGVKKSSKSRALTRPSMPRVPAASNSTSAKLVHTNNPRVPKKLR
KPAKPLLSKIRLRNHCKRLDQKSASRKLEMGSLVLKEPKVVLYKNLPIKKEREPEGPAHAAVGSGCLTRH
AAREHRQSHGRGAHSQGDSLPCTYTTRRSLRTRTGLKETTDIKLEPSPLDGYKNGILEPCPDSGQQPTPE
VLEELAPETAHREEASQECPKNDSCLSRKKFRQVKPVKHLAKTEDCSPEHSFPGKDGLPDLPGSHPDQGE
PSGTVRVPVSYTDSAPSPVGCSVVTPDSFTKDSFRTAQSKKKRRVTRYDAQLILENSSGIPKLTLRRRHD
SSSKTNDHESDGVNSSKISIKLSKDHDSDSNLYVAKLSNGVSAGPGSSSTKLKIQLKRDEESRGPCAEGL
HENGVCCSDPLSLLESQMEVDDYSQYEEDSTDESSSSSGKAAEANCW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC075709
ORF Size 2451 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC075709.1
RefSeq Size 3901 bp
RefSeq ORF 2453 bp
Locus ID 225888
MW 143 kDa
Gene Summary Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Plays a role in myogenesis by regulating the expression of target genes, such as EID3.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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