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Afg3l2 (NM_027130) Mouse Tagged ORF Clone
CAT＃: MR210733
- TrueORF®
Afg3l2 (Myc-DDK-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2)
ORF Plasmid: tGFP
"NM_027130" in other vectors (4)
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CNY 5,888.00
CNY 7,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310036I02Rik; AW260507; Emv66; par |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210733 representing NM_027130
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCACCGCTGCCTGCTGCTGTGGAGCCGGGGCGGCTGCCGTCGCGGCCTTCCTCCCCTGCTCGTGC CCAGAGGTTGCCTGGGTCCGG[AT]CCGGCGGCCCTGCCTCCGTACGCTCTATCAATATGCTACTGTCCA GACAGCAAGCAGCAGGCGTTCTCTGCTGAGGGATGTAATTGCTGCTTATCAAAGATTCTGTTCTCGACCT CCCAAAGGATTTGAAAAGTACTTTCCTAATGGGAAAAACGGAAAAAAGGCCAGTGAGCCTAAGGAGGCTG TTGGAGAAAAAAAAGAACCACAGCCCTCGGGCCCCCAGCCTTCTGGAGGTGCAGGTGGTGGGGGAGGGAA GCGCCGTGGCAAGAAAGAAGATTCTCACTGGTGGTCCAGGTTCCAGAAGGGTGACTTCCCATGGGATGAC AAGGATTTCAGGATGTACTTTCTCTGGACTGCTCTTTTTTGGGGTGGAGTCATGATTTACTTCGTGTTCA AGAGCTCTGGGAGAGAAATCACGTGGAAAGACTTTGTCAATAACTATCTTTCTAAGGGCGTGGTGGACAG ACTAGAAGTTGTCAACAAGCGTTTTGTTCGTGTGACCTTTACACCAGGAAAAACTCCGGTTGATGGGCAA TACGTCTGGTTTAATATTGGCAGTGTTGACACATTTGAGCGGAATCTGGAGACTTTGCAGCAAGAATTGG GCATAGAAGGGGAGAACCGGGTCCCTGTGGTTTATATTGCTGAGAGCGATGGCTCCTTCCTGCTGAGCAT GTTGCCCACCGTACTCATTATCGCTTTTCTACTCTACACCATAAGAAGAGGGCCCGCTGGCATTGGTCGG [AT]CCGGCCGGGGAATGGGTGGACTCTTCAGCGTTGGGGAAACCACAGCCAAGGTCTTAAAGGATGAGA TAGATGTGAAGTTTAAAGATGTGGCTGGCTGTGAGGAGGCCAAGCTAGAAATAATGGAATTCGTGAATTT CTTGAAAAACCCAAAGCAATATCAAGACCTAGGAGCAAAAATCCCAAAGGGTGCCATTCTCACCGGTCCC CCAGGTACTGGGAAGACGCTGCTAGCTAAGGCCACAGCTGGAGAAGCCAATGTCCCCTTTATCACTGTGA GCGGATCTGAGTTTCTGGAGATGTTTGTTGGCGTTGGTCCAGCCAGAGTCCGAGACTTATTTGCCCTTGC TCGGAAGAATGCGCCTTGCATTCTCTTCATTGATGAGATTGATGCTGTGGGAAGGAAGCGCGGCAGAGGC AACTTCGGTGGGCAGAGCGAGCAGGAGAACACACTCAACCAGCTGCTTGTGGAGATGGACGGCTTCAACA CAACCACCAATGTGGTCATCCTGGCAGGCACAAATCGACCAGACATCCTGGATCCAGCTCTGTTGAGACC AGGCCGCTTTGACAGGCAGATTTTTATTGGACCCCCAGACATAAAAGGACGAGCCTCAATCTTCAAAGTT CACCTTCGACCATTGAAGCTGGACAGTGCCTTGGAAAAAGATAAATTGGCCAGAAAACTGGCGTCCTTAA CTCCAGGGTTTTCAGGCGCTGATGTTGCCAATGTCTGCAATGAAGCTGCTTTGATTGCTGCAAGACACCT TTCAGATGCCATTAATGAGAAGCACTTCGAACAAGCGATTGAGCGAGTGATTGGAGGCTTGGAGAAAAAA ACCCAAGTTCTGCAGCCTGAGGAGAAGAAGACGGTGGCTTACCACGAAGCAGGCCATGCGGTCGCTGGCT GGTATCTGGAGCATGCAGACCCACTCTTAAAGGTCTCCATCATCCCGCGTGGCAAGGGGCTGGGCTATGC TCAGTACTTGCCCAAGGAGCAGTATCTGTACACAAAGGAGCAGCTGCTGGACAGGATGTGCATGACTCTG GGCGGCCGTGTCTCCGAGGAGATCTTCTTTGGGAGAATTACAACCGGTGCCCAGGACGACTTGAGGAAGG TTACCCAGAGTGCCTATGCCCAGATCGTTCAGTTTGGCATGAACGAGAAAGTGGGGCAGATCTCCTTTGA CCTCCCACGACAGGGGGACATGGTGTTAGAGAAGCCTTACAGTGAGGCCACTGCGAGGATGATAGACGAT GAAGTGAGGATACTCATCAGCGATGCCTACAGAAGGACGGTGGCTCTTCTCACAGAGAAGAAGGCTGACG TGGAGAAGGTCGCTCTCTTACTGTTAGAAAAGGAAGTCCTAGACAAGAATGACATGGTCCAGCTTCTCGG TCCCAGACCATTTACAGAAAAGTCCACATATGAAGAATTTGTGGAAGGCACTGGCAGCTTAGACGAGGAC ACTTCTCTTCCTGAAGGCCTTCAGGATTGGAACAAGGAGCGGGAGAAGGAGGAGAAGAAGGAGAAGGAGA AGGAGGAGCCGCTGAATGAGAAGGTTGTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210733 representing NM_027130
Red=Cloning site Green=Tags(s) MAHRCLLLWSRGGCRRGLPPLLVPRGCLGPXXRRPCLRTLYQYATVQTASSRRSLLRDVIAAYQRFCSRP PKGFEKYFPNGKNGKKASEPKEAVGEKKEPQPSGPQPSGGAGGGGGKRRGKKEDSHWWSRFQKGDFPWDD KDFRMYFLWTALFWGGVMIYFVFKSSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQ YVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGR XXGRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGP PGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRG NFGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKV HLRPLKLDSALEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDAINEKHFEQAIERVIGGLEKK TQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTL GGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARMIDD EVRILISDAYRRTVALLTEKKADVEKVALLLLEKEVLDKNDMVQLLGPRPFTEKSTYEEFVEGTGSLDED TSLPEGLQDWNKEREKEEKKEKEKEEPLNEKVVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027130 |
| ORF Size | 2412 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027130.1, NP_081406.1 |
| RefSeq Size | 3086 bp |
| RefSeq ORF | 2409 bp |
| Locus ID | 69597 |
| UniProt ID | Q8JZQ2 |
| MW | 90 kDa |
| Gene Summary | ATP-dependent protease which is essential for axonal and neuron development (PubMed:18337413, PubMed:27642048). In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU (By similarity). Required for the maturation of paraplegin (SPG7) after its cleavage by mitochondrial-processing peptidase (MPP), converting it into a proteolytically active mature form.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221711 | Afg3l2 (untagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2), (10ug) |
CNY 10,260.00 |
|
| MG210733 | Afg3l2 (tGFP-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2) |
CNY 7,700.00 |
|
| MR210733L3 | Lenti ORF clone of Afg3l2 (Myc-DDK-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2) |
CNY 8,930.00 |
|
| MR210733L4 | Lenti ORF clone of Afg3l2 (mGFP-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2) |
CNY 8,930.00 |
