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Ube3b (BC034059) Mouse Tagged ORF Clone
CAT＃: MR210662
- TrueORF®
Ube3b (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3B (cDNA clone MGC:31117 IMAGE:4163877)
ORF Plasmid: tGFP
"BC034059" in other vectors (3)
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CNY 7,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI449831; AU020130 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210662 representing BC034059
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGTAAATTCATCGTCTTCCTGAGAGATAGAGACCGATGCCGCGATGCTTGTGAGAGTTTAGAAG GCTGCCATACCCTGTGTCTCATGGGCAATCTCCTGCACTTGGGCTCCCTCAGCCTCAGGCTGTTAGAGGA AGAGATGGATGGGTTTGTGAGCGCGCTCACGCAGATGCTGTGCTACTGTCAGAAGTACGTGGCTCAGAAG AAGTCCAACCTGACCCACTGGCACCCTGTGCTCGGCTGGTTTTCCCAACCTGTGGATTACGGCCTCAATG ATTCCATGTACCTGATCACCAAGCAGCTGCAGTTCCTGTGGGCGGTGCCTCTCATCCGCATTCTCTTCAG TGACATCCTGAGCAGGAAGCTGCTGGAGCATGCAGAGCCGGCCCCGGTGCAACCGCAGCCGTCCTCACCG CAGACGGTGCTCCCCGTGAAGAGCCTCCTCAAGCGTGCGTTTCAGAAGTCGGCTTCGGTTCGGAACATCC TCAGGCCTGTTGGGGGCCGCCGTGTCGACTCCGCTGAGGTGCGGAAGGTCTGCAACATTTGCGTGCTCTA CCAGACCTCGCTGACGACCCTCACCCAGATCCGCCTGCAGATCCTCACAGGTCTTACGTACCTGGATGAC CTGCTGCCCAAGCTCTGGGCGTTTATCTGTGAGCTGGGGCCCCACGGCGGGCTCAAGCTCTTCCTGGAGT GCCTGAACAATGACACTGGAGAGTCCAAGCAGCTCCTGGCCATGCTGATGCTCTTCTGTGACTGTTCCCG GCACCTCATCACCATCCTCGATGACATTGAAGTTTATGAAGAACAGATCTCCTTCAAGCTGGAAGAGCTG GTCACCATCTCCTCGTTCCTCAATTCCTTCGTGTTTAAGATGATCTGGGACGGAATTGTAGAGAATGCCA AGGGTGAGACCTTGGAGCTGTTCCAGTCCGTCCATGGCTGGCTGATGGTGCTGTATGAGCGAGACTGCCG CCGGCGCTTTGCTCCGGAGGACCACTGGCTACGGAGGGACCTCAAGCCTGGTGTGCTCTTCCAGGAGCTC GACAAGGACCGAAGGAGGGCTCAGCTGGTCCTGCAGCACATCCCCCACGTCGTGCCTCACAAGAACAGAG TTCTTCTGTTCCGGAACATGGTTATCAAGGAGAAAGAGAAGCTGGGGCTTGTGGAGACCAGCTCTGCCTC TCCTCACGTCACCCACATCACCATCCGAAGGTCCCGGATGTTGGAGGACGGCTACGAACAGCTCCGGCAG CTTTCCCAGCATGCCATGAAGGGTGTGATCCGAGTGAAGTTTGTCAACGACCTCGGGGTGGACGAGGCTG GGATTGACCAGGATGGTGTCTTCAAGGAGTTTCTGGAAGAGATCATCAAGAGGGTGTTTGACCCCGCACT CAACCTGTTCAAGACAACCAGCGGGGATGAGCGGCTGTACCCATCACCCACGTCCTACATTCACGAGAAT TACCTGCAGCTCTTCGAGTTTGTGGGGAAGATGCTGGGAAAGGCAGTGTATGAGGGGATCGTGGTAGATG TGCCCTTTGCCTCCTTCTTCCTGAGCCAGATGCTCGGGCACCACCACAGCGTCTTCTACAGCTCCGTGGA TGAGCTTCCCTCCCTGGACTCGGAGTTTTATAAAAACCTCACCTCCATCAAGCGCTACGATGGAGACATC GCAGACCTCGGGCTGACGCTGTCTTACGATGAAGACGTCATGGGTCAGCTCGTCTGCCACGAACTGGTTC CCGGAGGGAAGACCATCCCGGTTACAGATGAGAACAAAATTAGCTACATCCATCTGATGGCGCATTTCCG AATGCACACTCAAATTAAAAACCAGACAGCTGCTCTTATCAGTGGCTTCCGTTCCATCATCAAGCCCGAG TGGATCCGAATGTTCTCTACCCCTGAGCTTCAGCGCCTCATCTCCGGGGACAACGCTGAGATAGATCTGG AAGACTTAAAGAAGCACACAGTGTACTACGGTGGCTTTCATGGGAGCCACAGGGTCATCATCTGGCTCTG GGATATCCTGGCTTCCGACTTCACGCCTGAAGAAAGAGCCATGTTCCTGAAGTTTGTGACCAGCTGCTCC CGGCCCCCGCTCCTAGGATTCGCCTACCTCAAGCCTCCGTTCTCCATCCGCTGTGTGGAAGTATCAGATG ACCAGGACACTGGGGATACCCTGGGCAGCGTCCTCAGGGGCTTCTTCACCATCCGCAAGAGGGAGCCAGG GGGGCGTCTGCCCACATCTTCCACTTGCTTCAACCTGCTTAAGCTGCCCAACTACAGCAAGAAGAGCGTA CTGCGAGAGAAACTACGCTATGCCATCAGCATGAACACGGGCTTTGAGCTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210662 representing BC034059
Red=Cloning site Green=Tags(s) MLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVAQK KSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPSSP QTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYLDD LLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEEL VTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQEL DKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQ LSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHEN YLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDI ADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPE WIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCS RPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSV LREKLRYAISMNTGFELS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034059 |
| ORF Size | 2364 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034059.1 |
| RefSeq Size | 4206 bp |
| RefSeq ORF | 2366 bp |
| Locus ID | 117146 |
| MW | 154.2 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210662 | Ube3b (tGFP-tagged) - Mouse ubiquitin protein ligase E3B (cDNA clone MGC:31117 IMAGE:4163877) |
CNY 8,650.00 |
|
| MR210662L3 | Lenti ORF clone of Ube3b (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3B (cDNA clone MGC:31117 IMAGE:4163877) |
CNY 9,790.00 |
|
| MR210662L4 | Lenti ORF clone of Ube3b (mGFP-tagged) - Mouse ubiquitin protein ligase E3B (cDNA clone MGC:31117 IMAGE:4163877) |
CNY 9,790.00 |
