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Pnpt1 (BC055826) Mouse Tagged ORF Clone
CAT＃: MR210634
- TrueORF®
Pnpt1 (Myc-DDK-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934)
ORF Plasmid: tGFP
"BC055826" in other vectors (4)
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CNY 5,736.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Old35, PNPase, RP23-313I4.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210634 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTGCAGGCTGTGCTGCTTGTGCCCGTGCCTCCGACCGCTGGGCTGCGGCCCCCTCGGCCGGC CCGGGCGGAATCGGGCACTCAGCTATTTGCAGATGCGAGCGTTGTGGAGCAGCACCGGATCCAGAGCGGT GACTGTAGACCTGGGACACAGAAAATTAGAAATATCCTCTGGGAAACTGGCAAGATTTGCTGATGGCTGT GCTGTGATACAGTCAGGCGATACTGCAGTAATGGTCACGGCAGTCAGCAAAACAAAAGCTTCGCCTTCCC AATTCATGCCGTTGGTGGTGGACTACCGACAGAAGGCTGCTGCAGCAGGCAGAATCCCCACAAACTACCT TAGAAGGGAGATTGGCTCCTCTGACAGAGAGGTTCTTACAAGTCGAGTAATAGATCGTTCAATTCGCCCT CTCTTTCCAGCCGGCTATTTTTATGATACTCAGGTTCTCTGTAATCTGCTAGCAGTAGATGGCATCAATG AACCTGATATCCTAGCAGTTAATGGTGCTTCTGTAGCCCTCTCCTTATCAGATATCCCTTGGAATGGACC TGTTGGGGCAGTACGAATAGGAATGATTGATGGAGAATGTGTCGTTAACCCAACAAGGAGAGAAATGTCT TCTAGCACTTTAAATTTAGTAGTTGCCGGAGCACCTAAAAGCCAAATTGTTATGTTGGAAGCCTCTGCAG AAAATATTCTACAGCAGGACTTTTGCCATGCTATCAAAGTTGGGGTGAAGTATACACAGCAGATAATTCA AGGCATCCAGCAGTTGGTAAAAGAAATCGGTGTTGCCAAGAGGACACCGCAGAAGATATTTACTCCTTCT GCAGAGATTGTGAAGTACACGAAGATAATTGCCATGGAGAAACTCTATGCGGTTTTTACAGATTATGAAC ATGATAAAGTTTCCAGGGATGAAGCTGTTAACAAGATAAGATTAGATACAGAGGAGCATCTAAAGGAAAA ATTTCCAGAGGTTGACCAATTTGAAATAATAGAATCCTTCAACATTGTTGCAAAGGAGGTTTTCCGAAGT ATTATTTTGAATGAATACAAAAGGTGTGATGGAAGAGATCTGACTTCACTTAGGAATATAAGTTGTGAGG TCGATATGTTTAAAACACTTCATGGATCAGCATTATTTCAGAGAGGACAAACACAGGTACTCTGTACTGT TACATTTGATTCATTAGAATCCAGTATTAAGTCAGATCAAATTATAACAGCTATAAATGGGGTAAAAGAT AAAAATTTCATGCTGCACTATGAGTTCCCTCCTTATGCAACCAATGAAACTGGCAAAGTTACTGGTGTGA ATCGAAGAGAACTTGGACATGGTGCTCTTGCCGAGAAAGCTTTGTGTCCTGTTATTCCCAAAGATTTTCC TTTTACCATAAGAGTTACATCTGAAGTCCTCGAATCAAATGGGTCATCTTCTATGGCATCTGCATGTGGT GGAAGTTTGGCATTAATGGATGCAGGGGTCCCAATTTCATCTGCTGTTGCAGGTGTAGCAGTGGGATTGG TTACCAAAACCAATCCAGAGAAAGGTGAAATAGAAGACTACCGTTTGCTAACAGATATTCTGGGAATTGA AGATTATAATGGTGACATGGATTTCAAAATAGCCGGTACAAATAAAGGAATAACTGCATTACAGGCTGAT ATTAAGTTACCTGGAGTACCAATTAAAATTATAATGGAAGCCATCCAACAAGCGTCAGTGGCAAAGAAGG AGATACTGCAGATAATGAACAAAACGATTTCAAAACCTCGAGCATCAAGAAAAGAAAATGGACCAGTTGT AGAAACAGTAAAGGTTCCATTATCAAAACGAGCAAAATTCGTTGGGCCTGGTGGATATCACTTAAAAAAA CTCCAGGCTGAGACAGGTGTAACAATTAGTCAGGTTGATGAAGAAACCTTCTCCATATTTGCACCAACAC CTACTGCAATGCATGAAGCAAGAGATTTCATTACAGAAATTTGCAGAGATGATCAAGAGCAACAATTAGA ATTTGGAGCAGTTTATACCGCGACAATAACTGAAATCAGAGACACTGGAGTGATGGTAAAACTGTATCCA AACATGACTGCAGTGCTGCTTCATAATTCACAACTTGACCAACGAAAGATTAAACATCCCACTGCCCTAG GACTAGAGGTTGGCCAAGAAATTCAGGTCAAATACTTTGGCCGTGATCCAGCTGATGGAAGAATGAGGCT TTCTCGTAAAGTACTTCAGTCTCCAGCTACAACTGCTCTCAAAACTCTAAATGATAGAAGCAGCATTGTA ATGGGAGAGCCTGTCTCACAGTCATCTAACTCTAACCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210634 protein sequence
Red=Cloning site Green=Tags(s) MAACRLCCLCPCLRPLGCGPLGRPGRNRALSYLQMRALWSSTGSRAVTVDLGHRKLEISSGKLARFADGC AVIQSGDTAVMVTAVSKTKASPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGSSDREVLTSRVIDRSIRP LFPAGYFYDTQVLCNLLAVDGINEPDILAVNGASVALSLSDIPWNGPVGAVRIGMIDGECVVNPTRREMS SSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKEIGVAKRTPQKIFTPS AEIVKYTKIIAMEKLYAVFTDYEHDKVSRDEAVNKIRLDTEEHLKEKFPEVDQFEIIESFNIVAKEVFRS IILNEYKRCDGRDLTSLRNISCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESSIKSDQIITAINGVKD KNFMLHYEFPPYATNETGKVTGVNRRELGHGALAEKALCPVIPKDFPFTIRVTSEVLESNGSSSMASACG GSLALMDAGVPISSAVAGVAVGLVTKTNPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQAD IKLPGVPIKIIMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVKVPLSKRAKFVGPGGYHLKK LQAETGVTISQVDEETFSIFAPTPTAMHEARDFITEICRDDQEQQLEFGAVYTATITEIRDTGVMVKLYP NMTAVLLHNSQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTALKTLNDRSSIV MGEPVSQSSNSNP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC055826 |
| ORF Size | 2349 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055826, AAH55826 |
| RefSeq Size | 2598 bp |
| RefSeq ORF | 2351 bp |
| Locus ID | 71701 |
| MW | 85.7 kDa |
| Gene Summary | RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Exploring the mitochondrial microRNA import pathway through Polynucleotide Phosphorylase (PNPase)
,null,
Journal of molecular and cellular cardiology
,PubMed ID 28709769
[Pnpt1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220827 | Pnpt1 (untagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934), (10ug) |
CNY 9,120.00 |
|
| MG210634 | Pnpt1 (tGFP-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) |
CNY 4,940.00 |
|
| MR210634L3 | Lenti ORF clone of Pnpt1 (Myc-DDK-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) |
CNY 6,370.00 |
|
| MR210634L4 | Lenti ORF clone of Pnpt1 (mGFP-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) |
CNY 6,370.00 |
