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Pnpt1 (BC055826) Mouse Untagged Clone
CAT＃: MC220827
Pnpt1 (untagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934), (10ug)
CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Old35, PNPase, RP23-313I4.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC055826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTGCAGGCTGTGCTGCTTGTGCCCGTGCCTCCGACCGCTGGGCTGCGGCCCCCTCGGCCGGC CCGGGCGGAATCGGGCACTCAGCTATTTGCAGATGCGAGCGTTGTGGAGCAGCACCGGATCCAGAGCGGT GACTGTAGACCTGGGACACAGAAAATTAGAAATATCCTCTGGGAAACTGGCAAGATTTGCTGATGGCTGT GCTGTGATACAGTCAGGCGATACTGCAGTAATGGTCACGGCAGTCAGCAAAACAAAAGCTTCGCCTTCCC AATTCATGCCGTTGGTGGTGGACTACCGACAGAAGGCTGCTGCAGCAGGCAGAATCCCCACAAACTACCT TAGAAGGGAGATTGGCTCCTCTGACAGAGAGGTTCTTACAAGTCGAGTAATAGATCGTTCAATTCGCCCT CTCTTTCCAGCCGGCTATTTTTATGATACTCAGGTTCTCTGTAATCTGCTAGCAGTAGATGGCATCAATG AACCTGATATCCTAGCAGTTAATGGTGCTTCTGTAGCCCTCTCCTTATCAGATATCCCTTGGAATGGACC TGTTGGGGCAGTACGAATAGGAATGATTGATGGAGAATGTGTCGTTAACCCAACAAGGAGAGAAATGTCT TCTAGCACTTTAAATTTAGTAGTTGCCGGAGCACCTAAAAGCCAAATTGTTATGTTGGAAGCCTCTGCAG AAAATATTCTACAGCAGGACTTTTGCCATGCTATCAAAGTTGGGGTGAAGTATACACAGCAGATAATTCA AGGCATCCAGCAGTTGGTAAAAGAAATCGGTGTTGCCAAGAGGACACCGCAGAAGATATTTACTCCTTCT GCAGAGATTGTGAAGTACACGAAGATAATTGCCATGGAGAAACTCTATGCGGTTTTTACAGATTATGAAC ATGATAAAGTTTCCAGGGATGAAGCTGTTAACAAGATAAGATTAGATACAGAGGAGCATCTAAAGGAAAA ATTTCCAGAGGTTGACCAATTTGAAATAATAGAATCCTTCAACATTGTTGCAAAGGAGGTTTTCCGAAGT ATTATTTTGAATGAATACAAAAGGTGTGATGGAAGAGATCTGACTTCACTTAGGAATATAAGTTGTGAGG TCGATATGTTTAAAACACTTCATGGATCAGCATTATTTCAGAGAGGACAAACACAGGTACTCTGTACTGT TACATTTGATTCATTAGAATCCAGTATTAAGTCAGATCAAATTATAACAGCTATAAATGGGGTAAAAGAT AAAAATTTCATGCTGCACTATGAGTTCCCTCCTTATGCAACCAATGAAACTGGCAAAGTTACTGGTGTGA ATCGAAGAGAACTTGGACATGGTGCTCTTGCCGAGAAAGCTTTGTGTCCTGTTATTCCCAAAGATTTTCC TTTTACCATAAGAGTTACATCTGAAGTCCTCGAATCAAATGGGTCATCTTCTATGGCATCTGCATGTGGT GGAAGTTTGGCATTAATGGATGCAGGGGTCCCAATTTCATCTGCTGTTGCAGGTGTAGCAGTGGGATTGG TTACCAAAACCAATCCAGAGAAAGGTGAAATAGAAGACTACCGTTTGCTAACAGATATTCTGGGAATTGA AGATTATAATGGTGACATGGATTTCAAAATAGCCGGTACAAATAAAGGAATAACTGCATTACAGGCTGAT ATTAAGTTACCTGGAGTACCAATTAAAATTATAATGGAAGCCATCCAACAAGCGTCAGTGGCAAAGAAGG AGATACTGCAGATAATGAACAAAACGATTTCAAAACCTCGAGCATCAAGAAAAGAAAATGGACCAGTTGT AGAAACAGTAAAGGTTCCATTATCAAAACGAGCAAAATTCGTTGGGCCTGGTGGATATCACTTAAAAAAA CTCCAGGCTGAGACAGGTGTAACAATTAGTCAGGTTGATGAAGAAACCTTCTCCATATTTGCACCAACAC CTACTGCAATGCATGAAGCAAGAGATTTCATTACAGAAATTTGCAGAGATGATCAAGAGCAACAATTAGA ATTTGGAGCAGTTTATACCGCGACAATAACTGAAATCAGAGACACTGGAGTGATGGTAAAACTGTATCCA AACATGACTGCAGTGCTGCTTCATAATTCACAACTTGACCAACGAAAGATTAAACATCCCACTGCCCTAG GACTAGAGGTTGGCCAAGAAATTCAGGTCAAATACTTTGGCCGTGATCCAGCTGATGGAAGAATGAGGCT TTCTCGTAAAGTACTTCAGTCTCCAGCTACAACTGCTCTCAAAACTCTAAATGATAGAAGCAGCATTGTA ATGGGAGAGCCTGTCTCACAGTCATCTAACTCTAACCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC055826 |
| Insert Size | 2352 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055826, AAH55826 |
| RefSeq Size | 2598 bp |
| RefSeq ORF | 2351 bp |
| Locus ID | 71701 |
| Gene Summary | RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210634 | Pnpt1 (tGFP-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) |
CNY 4,940.00 |
|
| MR210634 | Pnpt1 (Myc-DDK-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) |
CNY 5,736.00 |
|
| MR210634L3 | Lenti ORF clone of Pnpt1 (Myc-DDK-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) |
CNY 6,370.00 |
|
| MR210634L4 | Lenti ORF clone of Pnpt1 (mGFP-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) |
CNY 6,370.00 |
